Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application

Abstract

One of the greatest challenges of modern medicine is to find cheaper and easier ways to produce transporters for biologically active substances, which will provide selective and efficient drug delivery to the target cells, while causing low toxicity towards healthy cells. Currently, metal-based nanoparticles are considered a successful and viable solution to this problem. In this work, we propose the use of novel synthesis method of platinum nanoparticles (PtNPs) connected with their precise biophysical characterization and assessment of their potential toxicity. To work as an efficient nanodelivery platform, nanoparticles should interact with the desired active compounds spontaneously and non-covalently. We investigated possible direct interactions of PtNPs with ICR-191, a model acridine mutagen with well-established biophysical properties and mutagenic activity, by Dynamic Light Scattering, fluorescence spectroscopy, and Isothermal Titration Calorimetry. Moreover, to determine the biological activity of ICR-191-PtNPs aggregates, we employed Ames mutagenicity test, eukaryotic cell line analysis and toxicity test against the model organism Caenorhabditis elegans. PtNPs’ interesting physicochemical properties associated to the lack of toxicity in a tested range of concentrations, as well as their ability to modulate ICR-191 biological activity, suggest that these particles successfully work as potential delivery platforms for different biologically active substances.

Anisotropic Platinum Nanoparticle-Induced Cytotoxicity, Apoptosis, Inflammatory Response, and Transcriptomic and Molecular Pathways in Human Acute Monocytic Leukemia Cells

“…Increasing the ICR-191 concentration corresponded to decreasing cellular metabolic activity in non-cancerous HaCaT and cancerous MelJuSo cells incubated with PtNPs. In addition, ICR-191 has a more powerful impact on HaCaT than on MelJuSo cells [40]. PtNPs were established as having anticancer and cytotoxic activities against various type of cancerous and non-cancerous cells ha.…”

Poly(lactic-co-glycolic acid) encapsulated platinum nanoparticles for cancer treatment

“…52 Several studies have found that PtNPs of 50 nm have an anticancer effect, however other studies showed that PtNPs of 5 nm and 20 nm had no anticancer effect. 10,21,50,53,54 Therefore, in the present study, platinum nanoparticles were synthesized by chemical reduction following the methodology of Shim et al, to achieve a size of 50 nm. 50 The size, shape, and crystallinity of the PtNPs were determined by TEM and X-ray diffraction (XRD).…”

Acute Toxicity, Biodistribution, and Pharmacokinetics Studies of Pegylated Platinum Nanoparticles in Mouse Model

“…[ 5,12–17 ] However, exposure of PtNPs in a biological system might be associated with unanticipated hazards. [ 18–20 ] There are numerous reports on nephrotoxicity, hepatotoxicity, and inflammatory responses along with DNA damage induced by Pt‐based NPs. [ 21,22 ] On the other hand, PtNPs are also reported to inhibit pulmonary inflammation by exerting antioxidants and free radical scavenging activity.…”