Biophysical insight into the anti-amyloidogenic behavior of taurine

Chaturvedi, Sumit Kumar; Alam, Parvez; Khan, Javed Masood; Siddiqui, Mohd. Khursheed; Kalaiarasan, P.; Subbarao, Naidu; Ahmad, Zeeshan; Khan, Rizwan Hasan

doi:10.1016/j.ijbiomac.2015.06.035

Cited by 84 publications

(30 citation statements)

References 55 publications

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“…Prior to ThT fluorescence measurements, aliquots were supplemented with ThT (10 μM) and incubated for 30 minutes in dark. The obtained data was fitted against sigmoidal curve using equation in sigma plot [ 34 , 35 ]. where F is the fluorescence intensity at time t, and t 0 is the time to attain 50% of maximal fluorescence intensity.…”

Section: Methodsmentioning

confidence: 99%

Unraveling Comparative Anti-Amyloidogenic Behavior of Pyrazinamide and D-Cycloserine: A Mechanistic Biophysical Insight

et al. 2015

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Amyloid fibril formation by proteins leads to variety of degenerative disorders called amyloidosis. While these disorders are topic of extensive research, effective treatments are still unavailable. Thus in present study, two anti-tuberculosis drugs, i.e., pyrazinamide (PYZ) and D-cycloserine (DCS), also known for treatment for Alzheimer’s dementia, were checked for the anti-aggregation and anti-amyloidogenic ability on Aβ-42 peptide and hen egg white lysozyme. Results demonstrated that both drugs inhibit the heat induced aggregation; however, PYZ was more potent and decelerated the nucleation phase as observed from various spectroscopic and microscopic techniques. Furthermore, pre-formed amyloid fibrils incubated with these drugs also increased the PC12/SH-SY5Y cell viability as compare to the amyloid fibrils alone; however, the increase was more pronounced for PYZ as confirmed by MTT assay. Additionally, molecular docking study suggested that the greater inhibitory potential of PYZ as compare to DCS may be due to strong binding affinity and more occupancy of hydrophobic patches of HEWL, which is known to form the core of the protein fibrils.

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Section: Methodsmentioning

confidence: 99%

Unraveling Comparative Anti-Amyloidogenic Behavior of Pyrazinamide and D-Cycloserine: A Mechanistic Biophysical Insight

et al. 2015

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“…Taurine concentration in humans ranges from high millimolar in plasma, heart and brain, to micromolar amounts in tissue and body fluids such as urines (Schuller-Levis and Park 2003). Apart from its important physiological role as a neuroactive molecule (Wade et al 1988), it displays a wide range of pharmacological effects including membrane stabilization, cytoprotective effects, antioxidant and anti-inflammatory action (Chaturvedi et al 2015;Abdel-Moneim et al 2015). In addition taurine is able to restore muscle function and performance in different pathological conditions (Chan-Palay et al 1982a;Chan-Palay et al 1982c;Chan-Palay et al 1982b;De Luca et al 2015).…”

Section: Introductionmentioning

confidence: 99%

HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens

Francioso

Fanelli

Vigli

et al. 2017

Advances in Experimental Medicine and Biology

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There is an increasing interest for analytical methods aimed to detect biological sulfur-containing amines, because of their involvement in human diseases and metabolic disorders. This work describes an improved HPLC method for the determination of sulfur containing amino acids and amines from different biological matrices. We optimized a pre-column derivatization procedure using dabsyl chloride, in which dabsylated products can be monitored spectrophotometrically at 460 nm. This method allows the simultaneous analysis of biogenic amines, amino acids and sulfo-amino compounds including carnosine, dopamine, epinephrine, glutathione, cysteine, taurine, lanthionine, and cystathionine in brain specimens, urines, plasma, and cell lysates. Moreover, the method is suitable for the study of physiological and non-physiological derivatives of taurine and glutathione such as hypotaurine, homotaurine, homocysteic acid and S-acetylglutathione. The present method displays good efficiency of derivatization, having the advantage to give rise to stable products compared to other derivatizing agents such as o-phthalaldehyde and dansyl chloride.With this method, we provide a tool to study sulfur cycle from a metabolic point of view in relation to the pattern of biological amino-compounds, allowing researchers to get a complete scenario of organic sulfur and amino metabolism in tissues and cells.

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“…Congo red was dissolved in a 5 mM phosphate buffer (pH 7.0) containing 0.15 mM NaCl. The concentration of CR solution was determined using ɛ M : 45,000 M −1 cm −1 at 498 nm [33]. 0.2 mL 1-day-old samples containing 60% ethanol were mixed with 0.8 mL CR solution before the experiments.…”

Section: Congo Red Binding Assaymentioning

confidence: 99%

Grapefruit Seed Extract Inhibits the Formation of Amyloid-like Fibrils by Trypsin in Aqueous Ethanol

Kasi

Borics

Varga

et al. 2018

Natural Product Communications

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Several natural compounds deriving from plants are known to be efficient anti-amyloid aggregation agents. In this study, anti-aggregation activity of grapefruit seed extract was investigated using trypsin as a model protein in aqueous ethanol at pH 7.0. Using turbidity measurement, Congo red (CR) binding assay, electronic circular dichroism (ECD) and transmission electron microscopy (TEM), we found that grapefruit seed extract has ability to inhibit trypsin amyloidlike fibril formation in vitro, and effectiveness increases with growing concentration of grapefruit seed extract. The total phenolic content of it was determined. The results showed that in addition to the polyphenolic compounds some other compounds are also responsible for the fibril formation inhibitory effect. We indicated it for the first time that limonin has anti-fibrillation effect.

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Biophysical insight into the anti-amyloidogenic behavior of taurine

Cited by 84 publications

References 55 publications

Unraveling Comparative Anti-Amyloidogenic Behavior of Pyrazinamide and D-Cycloserine: A Mechanistic Biophysical Insight

Unraveling Comparative Anti-Amyloidogenic Behavior of Pyrazinamide and D-Cycloserine: A Mechanistic Biophysical Insight

HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens

Grapefruit Seed Extract Inhibits the Formation of Amyloid-like Fibrils by Trypsin in Aqueous Ethanol

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