Biophysical, spectroscopic and biochemical investigation of DNA–Cu(II)-GSH interactions

Abstract

The interaction of DNA with Copper(II)-Glutathione (CuGSH) has been investigated by various biophysical methods. The interaction ratio of DNA and Copper(II)-Glutathione in solution phase has been determined spectrophotometrically and found to be 0.25. EPR spectroscopy and UV-Vis findings suggest that Cu(II)-Glutathione neither bound to the DNA bases covalently nor intercalated, this has further been substantiated by the determination of intrinsic binding constant (2.

Probing the coordination properties of glutathione with transition metal ions (Cr2+,Mn2+,Fe2+,Co2+, Ni2+,Cu2+,Zn2+,Cd2+,Hg2+) by density functional theory

“…Different experimental techniques have been devoted to investigate the metal complexes of GSH, such as electron paramagnetic resonance (EPR) [26,27], NMR [28,29], X-ray diffraction (X-ray) [30,31] and ESI-MS [32]. Nevertheless, it is difficult to explain the structures of the complexes at the atomic and electronic level.…”