The interaction of DNA with Copper(II)-Glutathione (CuGSH) has been investigated by various biophysical methods. The interaction ratio of DNA and Copper(II)-Glutathione in solution phase has been determined spectrophotometrically and found to be 0.25. EPR spectroscopy and UV-Vis findings suggest that Cu(II)-Glutathione neither bound to the DNA bases covalently nor intercalated, this has further been substantiated by the determination of intrinsic binding constant (2.

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The interaction of calf-thymus DNA with copper(II)-cysteine (Cucys) was monitored by various biophysical methods. The interaction ratio was determined by the u.v.-spectrophotometric method, which was found to be 0.14. As the DNA 260 nm band remained insensitive followed by interaction with copper(II)-cysteine, the interaction ratio and the binding constant were determined by monitoring the 248 nm copper-cysteine charge-transfer (CT) band. Visible d-d spectra of Cucys proved that copper remained in the copper(II) state after interaction with DNA. The CD and thin film i.r. spectral studies revealed that there occurred B fi A conformational transition of DNA when it was bound by copper-cysteine. Gel electrophoresis study confirmed the binding pattern.

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