Enzymes have been used for more than 50 years in the detergent, textile, food industries, to name just a few. In these industries enzymes replace chemicals and minimize water, raw material and energy consumption. Natures own technology provides us with environmentally friendly solutions and better products. Enzymes are the natural solution to industrial problems. With enzymes we can maintain the living standards we have today and at the same time preserve the environment for our children. In textile industry, enzymes are used because: they are accelerating the reactions, act only on specific substrates, operate under mild conditions, are safe and easy to control, can replace harsh chemicals and are biologically degradable [1]. In textile manufacturing the use of enzymes has a long tradition. The enzymatic desizing of cotton with α-amylases is state-of-the-art since many decades'. Moreover, cellulases, pectinases, hemicellulases, lipases and catalases are used in different cotton pre-treatment and finishing processes [2]. These studies will overview the whole clean line of cotton fabrics wet processing arrangement avoiding usage of chemicals and substituting it with enzymes.