Bioprinting of Stem Cells in Multimaterial Scaffolds and Their Applications in Bone Tissue Engineering

Tharakan, Shebin; Khondkar, Shams; Ilyas, Azhar

doi:10.3390/s21227477

Cited by 30 publications

(15 citation statements)

References 124 publications

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“…MSC isolated from a variety of tissues have been isolated and then often incorporated into synthetic scaffolds, scaffolds with other ECM-like matrix components, an endogenous natural protein matrix or a hybrid synthetic/natural matrix, reviewed in [ 156 , 157 , 158 , 159 ]. Recent advances in bioprinting may offer more sophisticated and complex scaffold-cell constructs [ 160 , 161 , 162 ]. Such constructs are then implanted into defects in tissues or in an injury site.…”

Section: Use Of Msc In Tissue Engineered Constructs To Enhance Repair...mentioning

confidence: 99%

Creating an Optimal In Vivo Environment to Enhance Outcomes Using Cell Therapy to Repair/Regenerate Injured Tissues of the Musculoskeletal System

Hart

Nakamura

2022

Biomedicines

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Following most injuries to a musculoskeletal tissue which function in unique mechanical environments, an inflammatory response occurs to facilitate endogenous repair. This is a process that usually yields functionally inferior scar tissue. In the case of such injuries occurring in adults, the injury environment no longer expresses the anabolic processes that contributed to growth and maturation. An injury can also contribute to the development of a degenerative process, such as osteoarthritis. Over the past several years, researchers have attempted to use cellular therapies to enhance the repair and regeneration of injured tissues, including Platelet-rich Plasma and mesenchymal stem/medicinal signaling cells (MSC) from a variety of tissue sources, either as free MSC or incorporated into tissue engineered constructs, to facilitate regeneration of such damaged tissues. The use of free MSC can sometimes affect pain symptoms associated with conditions such as OA, but regeneration of damaged tissues has been challenging, particularly as some of these tissues have very complex structures. Therefore, implanting MSC or engineered constructs into an inflammatory environment in an adult may compromise the potential of the cells to facilitate regeneration, and neutralizing the inflammatory environment and enhancing the anabolic environment may be required for MSC-based interventions to fulfill their potential. Thus, success may depend on first eliminating negative influences (e.g., inflammation) in an environment, and secondly, implanting optimally cultured MSC or tissue engineered constructs into an anabolic environment to achieve the best outcomes. Furthermore, such interventions should be considered early rather than later on in a disease process, at a time when sufficient endogenous cells remain to serve as a template for repair and regeneration. This review discusses how the interface between inflammation and cell-based regeneration of damaged tissues may be at odds, and outlines approaches to improve outcomes. In addition, other variables that could contribute to the success of cell therapies are discussed. Thus, there may be a need to adopt a Precision Medicine approach to optimize tissue repair and regeneration following injury to these important tissues.

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Section: Use Of Msc In Tissue Engineered Constructs To Enhance Repair...mentioning

confidence: 99%

Creating an Optimal In Vivo Environment to Enhance Outcomes Using Cell Therapy to Repair/Regenerate Injured Tissues of the Musculoskeletal System

Hart

Nakamura

2022

Biomedicines

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“…Generally, higher viscous bioinks extruded from nozzles results in higher shear-stress, which is detrimental to cell viabilities. Additionally, the printing resolution is limited, with the range between 200 and 1000 µm, compared with other technologies [ 64 ].…”

Section: D Printing Technologies For Hard Tissue Replacementmentioning

confidence: 99%

Functional engineering strategies of 3D printed implants for hard tissue replacement

Chen

Huang

Liu

et al. 2022

Regenerative Biomaterials

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3D printing technology with the rapid development of printing materials are widely recognized as a promising way to fabricate bioartificial bone tissues. In consideration of the disadvantages of bone substitutes, including poor mechanical properties, lack of vascularization and insufficient osteointegration, functional modification strategies can provide multiple functions and desired characteristics of printing materials, enhance their physicochemical and biological properties in bone tissue engineering. Thus, this review focuses on the advances of functional engineering strategies for 3D printed biomaterials in hard tissue replacement. It is structured as introducing 3D printing technologies, properties of printing materials (metals, ceramics and polymers) and typical functional engineering strategies utilized in the application of bone, cartilage and joint regeneration.

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“…Cells, growth factors, and scaffolds are a necessary triad for tissue engineering. Despite advances in scaffold development and the identification of new growth factors and their genetic manipulation [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ], delivering sufficient numbers of cells and maintaining them in areas of interest, regardless of whether for ex vivo tissue engineering or in situ regeneration, has been challenging [ 1 , 12 , 15 , 16 , 17 , 18 , 19 , 20 ]. The effective delivery of stem cells or osteogenic cells derived from bone marrow or other source organs has been investigated in the context of tissue engineering and regeneration [ 12 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ].…”

Section: Introductionmentioning

confidence: 99%

“…The effective delivery of stem cells or osteogenic cells derived from bone marrow or other source organs has been investigated in the context of tissue engineering and regeneration [ 12 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ]. Various methods to improve cellular delivery have also been examined, including cell sheet technology, injectable vehicles, carrier molecules, and 3-dimensional (3D) cell printing [ 2 , 27 , 29 , 46 , 47 , 48 , 49 , 50 , 51 , 52 ]. Despite some progress, cell-based repair and regeneration of tissues, particularly fractured and diseased bone remains far from being a standard clinical modality, with the approach hampered by poor clinical viability, high costs, and suboptimal biological efficacy [ 37 , 38 , 47 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Cell Delivery System Exploiting Synergy between Fresh Titanium and Fibronectin

Hirota

Hori

Sugita

et al. 2022

Cells

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Delivering and retaining cells in areas of interest is an ongoing challenge in tissue engineering. Here we introduce a novel approach to fabricate osteoblast-loaded titanium suitable for cell delivery for bone integration, regeneration, and engineering. We hypothesized that titanium age influences the efficiency of protein adsorption and cell loading onto titanium surfaces. Fresh (newly machined) and 1-month-old (aged) commercial grade 4 titanium disks were prepared. Fresh titanium surfaces were hydrophilic, whereas aged surfaces were hydrophobic. Twice the amount of type 1 collagen and fibronectin adsorbed to fresh titanium surfaces than aged titanium surfaces after a short incubation period of three hours, and 2.5-times more fibronectin than collagen adsorbed regardless of titanium age. Rat bone marrow-derived osteoblasts were incubated on protein-adsorbed titanium surfaces for three hours, and osteoblast loading was most efficient on fresh titanium adsorbed with fibronectin. The number of osteoblasts loaded using this synergy between fresh titanium and fibronectin was nine times greater than that on aged titanium with no protein adsorption. The loaded cells were confirmed to be firmly attached and functional. The number of loaded cells was strongly correlated with the amount of protein adsorbed regardless of the protein type, with fibronectin simply more efficiently adsorbed on titanium surfaces than collagen. The role of surface hydrophilicity of fresh titanium surfaces in increasing protein adsorption or cell loading was unclear. The hydrophilicity of protein-adsorbed titanium increased with the amount of protein but was not the primary determinant of cell loading. In conclusion, the osteoblast loading efficiency was dependent on the age of the titanium and the amount of protein adsorption. In addition, the efficiency of protein adsorption was specific to the protein, with fibronectin being much more efficient than collagen. This is a novel strategy to effectively deliver osteoblasts ex vivo and in vivo using titanium as a vehicle.

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Bioprinting of Stem Cells in Multimaterial Scaffolds and Their Applications in Bone Tissue Engineering

Cited by 30 publications

References 124 publications

Creating an Optimal In Vivo Environment to Enhance Outcomes Using Cell Therapy to Repair/Regenerate Injured Tissues of the Musculoskeletal System

Creating an Optimal In Vivo Environment to Enhance Outcomes Using Cell Therapy to Repair/Regenerate Injured Tissues of the Musculoskeletal System

Functional engineering strategies of 3D printed implants for hard tissue replacement

A Novel Cell Delivery System Exploiting Synergy between Fresh Titanium and Fibronectin

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