Bioprinting with pre-cultured cellular constructs towards tissue engineering of hierarchical tissues

Abstract

The fabrication of physiologically active tissue constructs from various tissue elements are vital for establishing integrated bioprinting and transfer printing techniques as vital tools for biomedical research. Physiologically functional tissues are hierarchically constructed from a variety of tissue subunits with different feature sizes and topographies. For example, skeletal muscles are composed of many muscle bundles, muscle fibers, and muscle cells respectively. The fundamental constituents of all types of muscle tissues include various sized blood vessels, and vascular related cells. Nature has designed the direction of all the aforementioned components to have unidirectional alignment, so that muscle contractions can effectively generate the mechanical functions. In this study, we demonstrate a promising approach to fabricating such hierarchical tissues by applying bioprinting and a transfer patterning technique. Linear-patterned smooth muscle cells were obtained by culturing on the surface patterned discs, before being transferred onto the Matrigel substrate. The fiber-like tissues structures were successfully formed on the substrate after a few days of culturing; these are partially aligned smooth muscle cells. Additionally, stacked structures were also successfully fabricated using laminating printing technique. Our results indicate that bioprinting and transfer printing strategy of pre-cultured aligned muscular fiber-like tissues is very promising method to assemble tissue elements for biofabrication of hierarchical tissues.

Development of a 3D bio-printer using CoreXY mechanism and syringe-based extrusion

“…These facts are also support by the journal published by M. Calado et.al [18]. C. Ventola et.al [19] stated that 3D bio-printing are very famous especially in medical fields for research and study in many fields of researches such as tissue engineering, regenerative medicine and organ transplant since 2000s. The first 3D bio-printer [ 1181 is introduced by Wake Forest Institute in year 1999 in which a fully functioned artificial urinary bladder was successfully printed.…”

A Mathematical Model on the Resolution of Extrusion Bioprinting for the Development of New Bioinks

“…It has brought many benefits to tissue engineering and other biomedical applications by fabricating customized tissue constructs and prostheses [ 1 , 2 , 3 ]. A typical bioprinting system consists of three components including: (i) living materials which mostly refer to cells; (ii) hydrogel or bioink; and (iii) a bioprinter [ 4 , 5 ]. For bioprinters, there are mainly three types: laser-based bioprinters, ink-jet-based bioprinters, and extrusion-based bioprinters [ 6 , 7 ].…”

3D Printed Bioelectronic Platform with Embedded Electronics

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