Bioprocess development for production of a process-apt xylanase with multifaceted application potential for a range of industrial processes

Abstract

Discovery of new/novel enzymes with process-apt features has been a continuous practice. A wide range pH-stable and thermostable xylanase was reported hitherto from a newly isolated fungal strain Aspergillus terreus S9, from mushroom compost. This is the first ever report where-in a pH-stable and thermostable xylanase is being reported from a strain of A. terreus. Solid state fermentation was executed on agro-industrial residues for xylanase production. Maximum xylanase production was supported by wheat bran (40 U/ml), however, substantial xylanase yield was observed on other residues like corn cob powder (38.4 U/ml), cotton cake (38 U/ml), malt (37.6 U/ml) and almond hulls (35.2 U/ml). Almond hulls are being reported for the first time as the substrate for xylanase production. Plackett-Burman design was used to earmark the most effective process variables (wheat bran, medium pH, and incubation time), and the same were optimized by design of experiments (DoE) based on response surface methodology to achieve a yield enhancement of 1.82-fold. A. terreus S9 xylanase exhibited an excellent and brisk clarification of mosambi and orange juices. DoE based optimization of process parameters (enzyme dose, treatment time and incubation temperature) resulted in 1.23-fold and 1.28-fold enhanced orange and mosambi juice clarification, respectively. Furthermore, admirable dough raising capacity and pulp brightening ability of A. terreus S9 xylanase shows its promising potential for bread making, and eco-friendly biobleaching processes, respectively. The xylanase must be in-depth investigated to decipher the molecular mechanisms of diverse applications.

Bioprocess development for the production of xylooligosaccharide prebiotics from agro-industrial lignocellulosic waste

“…Since the enzymatic process operates under mild reaction conditions without the use of hazardous chemicals, it is more conducive to the idea of a biodegradable process for XOS production. Furthermore, the utilization of xylanolytic enzymes exhibits high specificity and efficiency, enabling a greater control over degree of polymerization and a decreased formation of unwanted xylose and other by-products [ 23 , 58 , 107 ]. Some of the most commonly used hydrolases for plant cell wall disintegration are cellulase, laccase, β-glucosidase, xylanases, and mannanase.…”

“…The second step, which is enzymatic saccharification, involves xylan hydrolysis with the help of xylanolytic enzymes such as endo-1,3-β-xylanases and endo-1,4-β-xylanases [ 108 ]. The xylose-releasing enzymes or the endo -xylanases, exo-xylanase/β-xylosidase and enzymes reducing the degree of polymerization of xylose or xylans have been known to be isolated from fungal and bacterial strains [ 23 , 58 , 107 ]. Enzyme preparations with low exo-xylanase and/or β-xylosidase activity are preferred for preventing the production of xylose.…”

Thermostable enzyme research advances: a bibliometric analysis

“…Cellulase and xylanase are the thermostable enzymes that are involved in this industrial sector [4,5,12]. The usefulness of thermostable cellulases and xylanases is primarily determined by their productivity, thermostability, specific activity, broad pH range and broad substrate specificity.…”

“…As a result, several industries are now taking steps to transition from chemical-based manufacturing to clean biological manufacturing. [3][4][5]. Enzymes are proteins that operate as biological catalysts in biological systems, speeding up reactions and catalyzing chemical reaction functions [6][7][8].…”

Valorization of Lignocellulosic Biomass into Biofuels and Value-Added Products for Sustainable Environment: A Comprehensive Review

“…Besides cellulases, hemicellulases such as β-xylosidase, xylanases, glucomannanase, glucuronidase, galactomannanase, and acetylesterase are employed for the hydrolysis of hemicellulose polymers [22,96,97]. Xylanases solubilize β-1,4-D-xylan fractions of hemicellulose by cleaving β-1,4-D-xylopyranosyl bond, and thus, results in the formation of oligomeric (xylo-oligosaccharides), and monomeric sugars [98,99].…”