Bioprocess for phytase production by Ganoderma sp. MR-56 in different types of bioreactors through submerged cultivation

Bioprocessing of Agricultural Residues as Substrates and Optimal Conditions for Phytase Production of Chestnut Mushroom, Pholiota adiposa, in Solid State Fermentation

“…Our results indicate that the chestnut mushroom, P. adiposa , possessed the greatest phytase producing strains, while water hyacinth was found to be the most suitable substrate for phytase production. These results are similar to those of previous studies [ 18 , 38 , 39 ] which reported that pure cultures of mushrooms could produce phytase after being cultured with agricultural residues under SSF. Notably, the efficiency of phytase production varied among different mushroom species and strains and was dependent upon the type of agricultural residues being used [ 18 , 19 , 38 , 39 , 40 , 41 ].…”

“…These results are similar to those of previous studies [ 18 , 38 , 39 ] which reported that pure cultures of mushrooms could produce phytase after being cultured with agricultural residues under SSF. Notably, the efficiency of phytase production varied among different mushroom species and strains and was dependent upon the type of agricultural residues being used [ 18 , 19 , 38 , 39 , 40 , 41 ]. A study conducted by Spier et al [ 19 ] found that the phytase production of S. commune DRL−01, T. versicolor DRL−18, G. applanatum DRL−56, and G. stipitatum DRL−70 on wheat bran under SSF ranged from 9.15 to 192.42 U/gds.…”

Bioprocess for Production, Characteristics, and Biotechnological Applications of Fungal Phytases

“…Neurospora sitophila showed the highest level of phytase production at 195.66 U/g followed by A. niger and R. oryzae. However, not only SSF has been investigated for phytase production, but a number of research studies have also investigated phytase production involving SmF and semi-solid fermentation methods (Han et al, 1987;Salmon et al, 2016;Shah et al, 2017). The production of phytase from several fungal strains has been investigated during SmF.…”

“…For example, phytase production from Aspergillus fumigatus, A. japonicus, A. niger, Muscodor sp., and Ganoderma sp. MR-56 was investigated under SmF conditions using wheat bran as a substrate (Mandviwala and Khire, 2000;Mohan et al, 2004;Alves et al, 2016;Maller et al, 2016;Salmon et al, 2016). Coban and Demirci (2014) have produced and optimized culture conditions for A. ficuum NRRL 3135 using phytase selective medium containing sodium phytate as a substrate.…”

“…Salmon et al (2012) reported that a maximal level of phytase (113.7 U/gds) was obtained in wheat branbased medium involving 50% humidity with 7.5% of the biomass at 33 • C and at a pH value of 7.0 over 72 h, which resulted in a 285% level of improvement in terms of the amounts of enzymes obtained. Ganoderma applanatum synthesized phytase in a medium comprised of soybean molasses as a carbon source and yeast extract as a nitrogen source at 30 • C at 150 rpm, a pH of 6.0 and a 3% inoculum rate through SmF (Salmon et al, 2016). The production of phytase by fungal strains has been observed at a wide range of initial pH values and temperatures ranging from 4.5-8.0 and 27-50 • C, respectively.…”