Bioprocess Optimization for Exopolysaccharides Production by Ganoderma lucidum in Semi-industrial Scale

Abstract

Background: For many years, ganoderma was highly considered as biofactory for the production of different types of bioactive metabolites. Of these bioactive compounds, polysaccharides gained many attentions based on their high biotherapeutic properties. Therefore, special attention have been paid during the last years for production of mushrooms bioactive compounds in closed cultivation system to shorten the cultivation time and increase the product yield. Results and Discussion: Therefore, this work is focused on the development of simple cultivation system for exopolysaccharides (EPS) production using Ganoderman lucidum. At first, the best medium supporting EPS production was chosen experimentally from the current published data. Second, like many EPS production process, carbon and nitrogen concentrations were optimized to support the highest production of polysaccharides in shake flask level. Furthermore, the process was scaled up in 16-L stirred tank bioreactor. The results clearly demonstrated that the best cultivation strategy was cultivation under controlled pH condition (pH 5.5). Under this condition the maximal volumetric and specific yield of EPS production were, 5.0 g/L and 0.42 g/g, respectively. Conclusion: The current results clearly demonstrates the high potential use of submerged cultivation system as alternative to conventional solid state fermentation for EPS production by G. lucidum. Furthermore, optimization of both carbon and nitrogen sources concentration and scaling up of the process showed significant increase in both volumetric and specific EPS production.

Exopolysaccharide Production in Submerged Fermentation of Pleurotus ostreatus under Red and Green Light

“…In addition, this study revealed that a controlled pH strategy improved EPS production and pleuran production with values of 1.98 g/L and 0.4 g/g to be achieved [43]. A higher EPS production compared to our study was achieved only for a Ganoderma lucidum EPS that was produced in a 16 L stirred tank bioreactor, with a production value of 5.0 g/L after fermentation in controlled pH conditions [44]. These two studies suggest that future fermentation of P. ostreatus using red light with a controlled pH strategy process could further increase EPS production.…”

Study the Effect of Different Renewable Carbon Sources on the Succinic Acid Biosynthesis by Optimization Culture Composition using 4-Liter Scale Bioreactor

“…The recovering process used 6000 rpm centrifugation for 10 minutes at 4C. This study required special extra supernatant utilization to estimate SA by using (HPLC) Waters 2690 Alliance Separations Module with a flow rate of (0.6) m/min, UV detection of (210) nm, Mobile Phase H 2 SO 4 of (0.005N), Waters 996 Photodiode Array Detector and Column: Hi-Plex H, 300  7.7 mm with guard column and temperature at 40C (Alsaheb et al, 2020).…”

“…Presumably, the sugar diminishes has occurred approximately between 36 h to 48 h. The quantity of dissolved oxygen (DO) decreased gradually from 100% to 25.00% at the end of fermentation. All the findings at the bioreactor stage could be explained by the effects of corn fiber (sugar complexity) and the production of many acids during the Actinobacillus succinogenes fermentation pathway (Alsaheb et al, 2020;Raje Nimbalkar et al, 2022).…”

Biotech Application of Exopolysaccharides from Curvularia brachyspora: Optimization of Production, Structural Characterization, and Biological Activity

“…In addition, these macromolecules are relatively non-toxic to normal cells and tissues, which affords no significant side effects if used as therapeutic drugs [3]. Based on such evidence, many studies about fungal polysaccharides, their sources, and how to produce them have been published, showing the potential of biotechnological Molecules 2023, 28, 4356 2 of 16 tools to obtain bioactive compounds [1][2][3][4][5]. Among the biological properties described for such macromolecules, it can be mentioned: antioxidant, anti-inflammatory, antiangiogenic, antiviral, antitumor, and immunomodulatory activities [6][7][8].…”