The three major polymeric components of Scots Pine sawdust–lignin, hemicellulose and cellulose–represent potential substrates for methanogenic fermentation although, in the absence of physicochemical pretreatment, low digestibility was demonstrated even after protracted incubation (18 months). Acid (H2SO4) and alkali (NaOH) pretreatments mediated different effects although, in general, the rates and percent solubilisations increased with concentration, temperature, pressure and time, either singly or in combination. Individual methanogenic fermentations with the hydrolysates and residual solids showed that although 3.3% more methane resulted from the H2SO4 hydrolysate than the corresponding NaOH hydrolysate, in total, 7% more methane was generated from the two alkali fractions than from the corresponding acid fractions.
The results thus exemplified that choice of a specific physicochemical strategy must be made in conjunction with the expected yield from the selected fraction(s).