Bioprocessing of wheat bran for the production of lignocellulolytic enzyme cocktail byCotylidia pannosaunder submerged conditions

Abstract

Characterization and production of efficient lignocellulytic enzyme cocktails for biomass conversion is the need for biofuel industry. The present investigation reports the modeling and optimization studies of lignocellulolytic enzyme cocktail production by Cotylidia pannosa under submerged conditions. The predominant enzyme activities of cellulase, xylanase and laccase were produced in the cocktail through submerged conditions using wheat bran as a substrate. A central composite design approach was utilized to model the production process using temperature, pH, incubation time and agitation as input variables with the goal of optimizing the output variables namely cellulase, xylanase and laccase activities. The effect of individual, square and interaction terms on cellulase, xylanase and laccase activities were depicted through the non-linear regression equations with significant R 2 and P-values. An optimized value of 20 U/ml, 17 U/ml and 13 U/ml of cellulase, xylanase and laccase activities, respectively, were obtained with a media pH of 5.0 in 77 h at 31C, 140 rpm using wheatbran as a substrate. Overall, the present study introduces a fungal strain, capable of producing lignocellulolytic enzyme cocktail for subsequent applications in biofuel industry.

Preparation and characterization of coffee hull fiber for reinforcing application in thermoplastic composites

“…The thermal degradation of the CH/HDPE composite took place in two phases from 260°C–395°C to 397°C–470°C. The mass loss in the first temperature ranges can be due to the thermal degradation of lignocellulosic components of CH mainly cellulose and lignin, respectively [ 34 ]. Thermal degradation in the second thermal range can be attributed to mass loss from breaking down of HDPE and some lignin components of CH and it also corresponds to the thermal degradation value of neat HDPE [ 35 ].…”

Hyper production of carboxy methyl cellulase by Thermomyces dupontii utilizing physical and chemical mutagenesis

“…Among all fermentation media maximum CMCase production was recorded in Medium3 this could be reason that wheat bran consist of carbohydrates and proteins. Wheat bran serves as an excellent carbon source due to its large surface area and rich nutritional content such as 27% total carbohydrate, 14% of protein, 64% digestible nitrogen and 6% of lipids (Sharma et al, 2016).…”

Application of Box–Behnken design for the optimization of cellulase production under solid-state fermentation

“…Wheat bran due to its nutritional content and large surface area serves to be an excellent carbon source without any supplementary carbon source for the production of lignocellulolytic enzymes [13]. XRD and FTIR pattern of wheat bran also suggest that cellulose present in wheat bran is easily available for microbial attack.…”

“…The soluble cello-oligosaccharides compositions of wheat bran were proved to be one of the most significant factors for cellulase production and it can also be utilized without any prior pretreatment [3,13].…”