Bioprospecting for podophyllotoxin in the Big Horn Mountains, Wyoming

Zheljazkov, Valtcho D.; Cantrell, Charles L.; Donegá, Mateus Augusto; Astatkie, Tess

doi:10.1016/j.indcrop.2012.08.024

Cited by 10 publications

(11 citation statements)

References 13 publications

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“…Renouard et al (2011), Gawde et al (2009, and Zheljazkov et al (2013) reported that PDP concentration in Juniperus sp. varied significantly depending on the geographic origin of the samples.…”

Section: Analysis Of Podophyllotoxin and Deoxypodophyllotoxin Contentmentioning

confidence: 99%

“…Different varieties of J. horizontalis, investigated by Zheljazkov et al (2013) and Cantrell et al (2013), showed concentration of PDP, ranging from 0% to 0.726%. In the present study, the investigated varieties of J. horizontalis were characterized with the PDP content ranging from 0.003% to 0.305%.…”

Section: Analysis Of Podophyllotoxin and Deoxypodophyllotoxin Contentmentioning

confidence: 99%

“…has been described in several papers, first published in 1953 by Hartwell et al In the majority of cases, the authors reported on the low levels of these lignans in the analyzed samples. Other researchers (Canel et al, 2000;Cantrell et al, 2013;Cushman et al, 2003;Gawde et al, 2009;Kusari et al, 2011;Renouard et al, 2011;Zheljazkov et al, 2013) investigated Juniperus virginiana L. and other species as an alternative source of PDP.…”

Section: Introductionmentioning

confidence: 99%

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Study of cytotoxic activity, podophyllotoxin, and deoxypodophyllotoxin content in selectedJuniperusspecies cultivated in Poland

Och

Cieśla³

et al. 2015

Pharmaceutical Biology

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Context: The demand for podophyllotoxin and deoxypodophyllotoxin is still increasing and commercially exploitable sources are few and one of them, Podophyllum hexandrum Royle (Berberidaceae), is a ''critically endangered'' species. Objective: The first aim was to quantify the amount of podophyllotoxin and deoxypodophyllotoxin in 61 Juniperus (Cupressaceae) samples. Cytotoxic activity of podophyllotoxin and ethanolic leaf extracts of Juniperus scopulorum Sarg. ''Blue Pacific'' and Juniperus communis L. ''Depressa Aurea'' was examined against different leukemia cell lines. Materials and methods: Ultra-performance liquid chromatography (UPLC) analysis was performed with the use of a Waters ACQUITY UPLC TM system (Waters Corp., Milford, MA). The peaks of podophyllotoxin and deoxypodophyllotoxin were assigned on the basis of their retention data and mass-to-charge ratio (m/z). Trypan blue assay was performed to obtain IC 50 cytotoxicity values against selected leukemia cell lines. Results: Juniperus scopulorum was characterized with the highest level of podophyllotoxin (486.7 mg/100 g DW) while Juniperus davurica Pall. contained the highest amount of deoxypodophyllotoxin (726.8 mg/100 g DW). Podophyllotoxin IC 50 cytotoxicity values against J45.01 and CEM/C1 leukemia cell lines were 0.0040 and 0.0286 mg/mL, respectively. Juniperus scopulorum extract examined against J45.01 and HL-60/MX2 leukemia cell lines gave the respective IC 50 values: 0.369-9.225 mg/mL. Juniperus communis extract was characterized with the following IC 50 cytotoxity values against J45.01 and U-266B1 cell lines: 3.310-24.825 mg/mL. Conclusions: Juniperus sp. can be considered as an alternative source of podophyllotoxin and deoxypodophyllotoxin. Cytotoxic activity of podophyllotoxin and selected leaf extracts of Juniperus sp. against a set of leukemia cell lines was demonstrated.

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Section: Analysis Of Podophyllotoxin and Deoxypodophyllotoxin Contentmentioning

confidence: 99%

Section: Analysis Of Podophyllotoxin and Deoxypodophyllotoxin Contentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Study of cytotoxic activity, podophyllotoxin, and deoxypodophyllotoxin content in selectedJuniperusspecies cultivated in Poland

Och

Cieśla³

et al. 2015

Pharmaceutical Biology

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“…Both species contain a significant amount of plant secondary metabolites. Rocky Mountain juniper contains podophyllotoxin and essential oil (Cantrell et al, 2013;Zheljazkov et al, 2013). Big sagebrush contains essential oil, MJ (Jassbi et al, 2010), and other plant chemicals.…”

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confidence: 99%

Effect of Foliar Application of Methyl Jasmonate and Extracts of Juniper and Sagebrush on Essential Oil Yield and Composition of ‘Native’ Spearmint

Zheljazkov¹,

Astatkie²,

Jeliazkova³

2013

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Spearmint (Mentha spicata L.) is a major essential oil crop in the United States. Developing means for increased biomass and oil yields and increased concentrations of carvone would be beneficial for the essential oil industry. A field experiment was conducted to study the effect of foliar application treatments [water (control), water + Tween20, sagebrush essential oil + Tween20, sagebrush water extract, juniper essential oil + Tween20, juniper water extract, and methyl jasmonate] on ‘Native’ spearmint essential oil content, shoot fresh weight, oil yield, and oil composition. The essential oil content was low in the water + Tween20 and in juniper essential oil (EO) + Tween20 treatments and high in the juniper water extract and in methyl jasmonate (MJ) treatments; however, neither of these were different from the water control or from the sagebrush EO + Tween20. Biomass fresh weight was lower in the sagebrush water extract (SWE) and in the juniper water extract (JWE) relative to the water control. The application of juniper EO + Tween20 reduced the concentration of carvone in the oil relative to the water control or to the water + Tween20 treatment. The sagebrush water extract, juniper EO + Tween20, juniper water extract, and MJ increased the concentrations of beta-caryophyllene relative to the water treatment. The concentration of trans-beta-farnesene was lower in the control water treatment and higher in all other treatments. Also, with the exception of sagebrush EO + Tween20, all treatments increased the concentration of germacrene D relative to the water control but not relative to water + Tween20. This study demonstrated that foliar application of sagebrush EO, sagebrush water extract, juniper EO, or juniper water extract to ‘Native’ spearmint may affect the essential oil profile of spearmint essential oil. Some of the treatments increased the concentrations of beta-caryophyllene, trans-beta-farnesene, and germacrene D in the essential oil; however, neither of the treatments increased the concentration of carvone, the main essential oil constituent of ‘Native’ spearmint oil.

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“…Although the concentration of podophyllotoxin in J. virginiana is much lower than that in Podophyllum species, J. virginiana is a perennial evergreen tree with much higher biomass production, greater ecological adaptability, and year-round availability. In addition, J. virginiana leaves (needles), containing podophyllotoxin, are widely available as one of the timber industry's by-products [1][2][3]. Podophyllotoxin is the preferred precursor for the semi-synthesis of the anti-cancer drugs etoposide, teniposide and etopophose.…”

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confidence: 99%

Plant Tissue Cultures of Juniperus virginiana

Kašparová

Spilková

Cvak³

et al. 2016

Natural Product Communications

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Callus cultures of Juniperus virginiana L. (varieties 'Hetzii', 'Glauca', 'Grey Owl') were derived from fresh leaves of garden-grown trees on Schenk and Hildebrandt medium supplemented with 3.0 mg/L of α-naphthaleneacetic acid, 0.2 mg/L of kinetin and 15 mg/L of ascorbic acid. The growth characteristics of one-year-old and two-years-old cultures were determined. The maximum biomass in all varieties was achieved on the 35 th day of the cultivation period. The increase in fresh weights of two-years-old callus cultures, when compared with one-year-old callus cultures, was as follows: variety 'Hetzii' by 25%, variety 'Glauca' by 29% and variety 'Grey Owl' by 49%. J. virginiana suspension cultures (varieties 'Hetzii', 'Glauca', 'Grey Owl') were derived from two-years-old callus cultures on Schenk and Hildebrandt medium supplemented with 3.0 mg/L of α-naphthaleneacetic acid, 0.2 mg/L of kinetin and 15 mg/L of ascorbic acid. The maximum biomass of all varieties was found on the 21 st day of the cultivation period. These results indicate that a sub-cultivation interval of 35 days for callus cultures and of 21 days for suspension cultures can be recommended. The callus and suspension cultures of J. virginiana of the variety 'Glauca' have the best survivability and thus provide the most biomass.

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Bioprospecting for podophyllotoxin in the Big Horn Mountains, Wyoming

Cited by 10 publications

References 13 publications

Study of cytotoxic activity, podophyllotoxin, and deoxypodophyllotoxin content in selectedJuniperusspecies cultivated in Poland

Study of cytotoxic activity, podophyllotoxin, and deoxypodophyllotoxin content in selectedJuniperusspecies cultivated in Poland

Effect of Foliar Application of Methyl Jasmonate and Extracts of Juniper and Sagebrush on Essential Oil Yield and Composition of ‘Native’ Spearmint

Plant Tissue Cultures of Juniperus virginiana

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