Study of cytotoxic activity, podophyllotoxin, and deoxypodophyllotoxin content in selected<i>Juniperus</i>species cultivated in Poland

Och, Marek; Och, Anna; Cieśla, Łukasz; Kubrak, Tomasz; Pecio, Łukasz; Stochmal, Anna; Kocki, Janusz; Bogucka‐Kocka, Anna

doi:10.3109/13880209.2014.943246

Cited by 30 publications

(26 citation statements)

References 27 publications

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“…These differences may lead to different responsiveness of J.45 and HL60 in the cytotoxicity tests [ 36 ]. A similar tendency (higher toxicity in relation to the J45 than HL60 lines) was observed in earlier studies [ 37 ]. Literature data confirm our results, indicatind that J.45 cells are more sensitive to chemopreventive agents, such as anthocyanins, than HL60 cells.…”

Section: Resultssupporting

confidence: 89%

Antioxidant, Anti-Inflammatory, and Postulated Cytotoxic Activity of Phenolic and Anthocyanin-Rich Fractions from Polana Raspberry (Rubus idaeus L.) Fruit and Juice—In Vitro Study

2018

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In this study, the antioxidative and anti-inflammatory potential of crude extracts (CE), anthocyanin-rich fractions (ARF), and phenolic fractions (PF) from raspberry (R) and raspberry juice (J) were evaluated. The antioxidant properties were evaluated with three complementary assays: DPPH radical scavenging activity, chelating Fe(II) power, and ferric reducing power. The highest antioxidant activity was determined for the crude extract from raspberry pulp (RCE) in the case of all methods used. The anti-inflammatory activity was demonstrated by inhibitory effect on lipoxygenase (LOX) and cyclooxygenase-2 (COX-2) activity in vitro. The highest efficiency in inhibiting the activity of both enzymes was exhibited by RCE, 0.79 and 0.59 mg FW/mL, respectively. In turn, JARF had the lowest ability to inhibit LOX (EC50 = 4.5 mg FW/mL) and JPF caused the lowest COX-2 inhibition (1.75 mg FW/mL). Additionally, we have performed a pilot study of in vitro cytotoxic activity using two human leukemia cell lines: J45 and HL60. All examined extracts inhibited the viability of J45 cells more effectively than HL60. The highest cytotoxic effect was observed in the J45.01 cell line after exposure to RCE (EC50 = 0.0375 mg FW/mL).

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Section: Resultssupporting

confidence: 89%

Antioxidant, Anti-Inflammatory, and Postulated Cytotoxic Activity of Phenolic and Anthocyanin-Rich Fractions from Polana Raspberry (Rubus idaeus L.) Fruit and Juice—In Vitro Study

2018

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“…In total, the present results exhibited significant consistency with previous reports on Juniperus plants. The content of podophyllotoxin is very high in Juniperus species and also has a good antitoxic effect . The hyperoside, amentoflavone, and formononetin are all well‐known human health antioxidants .…”

Section: Resultsmentioning

confidence: 99%

Chemical Composition and Antioxidant Activity of Essential Oils and Methanol Extracts of Different Parts from Juniperus rigida Siebold & Zucc.

Liu

Wang

et al. 2016

Chemistry & Biodiversity

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The chemical composition and antioxidant activity of essential oils and MeOH extracts of stems, needles, and berries from Juniperus rigida were studied. The results indicated that the yield of essential oil from stems (2.5%) was higher than from needles (0.8%) and berries (1.0%). The gas chromatography/mass spectrometer (GC/MS) analysis indicated that 21, 17, and 14 compounds were identified from stems, needles, and berries essential oils, respectively. Caryophyllene, α-caryophyllene, and caryophyllene oxide were primary compounds in both stems and needles essential oils. However, α-pinene and β-myrcene mainly existed in berries essential oils and α-ionone only in needles essential oils. The high-performance liquid chromatography (HPLC) analysis indicated that the phenolic profiles of three parts exhibited significant differences. Needles extracts had the highest content of chlorogenic acid, catechin, podophyllotoxin, and amentoflavone, and for berries extracts, the content of those compounds was the lowest. Meanwhile, three in vitro methods (DPPH, ABTS, and FRAP) were used to evaluate antioxidant activity. Stems essential oil and needles extracts exhibited the powerful antioxidant activity than other parts. This is the first comprehensive study on the different parts of J. rigida. The results suggested that stems and needles of J. rigida are useful supplements for healthy products as new resources.

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“…These cell lines represent different types of leukemia, namely, acute leukemia and myeloma. As a positive control podophyllotoxin was used; the results were published previously by Och et al [37]. …”

Section: Resultsmentioning

confidence: 99%

Phytochemical Content and Pharma‐Nutrition Study on Eleutherococcus senticosus Fruits Intractum

Załuski

Olech

Galanty

et al. 2016

Oxidative Medicine and Cellular Longevity

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In the past two decades public interest in herbal products has increased significantly in Europe, especially in the plant-based products from non-European traditions. Eleutherococcus senticosus has been used for the treatment of inflammatory diseases, anemia, and rheumatoid arthritis. The Eleutherococcus senticosus fruits intractum was examined for the content of phenolic acids (LC-ESI-MS/MS), minerals (AAS), TPC, and TFC (spectrophotometric assay). The antioxidant activity was determined using free radical scavenging assay and TLC-DB-DPPH∗ dot-blot test. An anti-Hyal activity was evaluated by the spectrophotometric assay method. Cytotoxicity towards HL-60, HL-60/MX1, HL-60/MX2, CEM/C1, and CCRF/CEM leukemic cell lines was done using trypan blue test. Among eight phenolic acids, trans-caffeic acid was found in the largest amount (41.2 mg/g DE). The intractum presented a high amount of macroelements (Ca, Mg, K; 1750, 1300, and 21000 mg/kg) and microelements (Fe, Mn; 32.7, 54.3 mg/kg), respectively. The content of TPC and TFC was 130 and 92 mg/g DE, respectively. The intractum showed anti-Hyal activity (2.16–60%) and an antioxidant capacity (EC50; 52 μg/mL). The intractum most strongly inhibited the growth of HL-60, HL-60/MX1, and CCRF/CEM. A better understanding of the intractum health benefits is important in order to increase its utility and enrich dietary sources of health promoting compounds.

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Study of cytotoxic activity, podophyllotoxin, and deoxypodophyllotoxin content in selectedJuniperusspecies cultivated in Poland

Cited by 30 publications

References 27 publications

Antioxidant, Anti-Inflammatory, and Postulated Cytotoxic Activity of Phenolic and Anthocyanin-Rich Fractions from Polana Raspberry (Rubus idaeus L.) Fruit and Juice—In Vitro Study

Antioxidant, Anti-Inflammatory, and Postulated Cytotoxic Activity of Phenolic and Anthocyanin-Rich Fractions from Polana Raspberry (Rubus idaeus L.) Fruit and Juice—In Vitro Study

Chemical Composition and Antioxidant Activity of Essential Oils and Methanol Extracts of Different Parts from Juniperus rigida Siebold & Zucc.

Phytochemical Content and Pharma‐Nutrition Study on Eleutherococcus senticosus Fruits Intractum

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