Bioprospecting of endophytic actinobacterium associated with Aloe ferox mill for antibacterial activity

Maliehe, Tsolanku Sidney; Mbambo, Melusi; Ngidi, Londeka Sibusisiwe; Shandu, Jabulani Siyabonga; Pooe, Ofentse Jacob; Masoko, Peter; Selepe, Tlou Nelson

doi:10.1186/s12906-022-03733-8

Cited by 5 publications

(2 citation statements)

References 55 publications

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“…Thus, the disturbance of the respiratory chain dehydrogenase can result in insufficient energy production and supply, consequently leading to the bacterial inhibition and/or death [ 43 ]. In this study, the extract showed destructive properties against the respiratory chain dehydrogenase, implying that the bacteria’s energy production pathway was affected, consequently leading to the inhibition and/or killing of the selected bacterial strains [ 44 ]. The results are in agreement with the findings by Gomaa, [ 45 ], whereby the antimicrobial compounds were able to exert their efficacies by tempering with the activity of bacterial respiratory chain dehydrogenase.…”

Section: Discussionmentioning

confidence: 99%

Chemical Profile, Antioxidant and Antibacterial Activities, Mechanisms of Action of the Leaf Extract of Aloe arborescens Mill

Maliehe

Nqotheni

Shandu

et al. 2023

Plants

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Aloe arborescens Mill’s extracts have been explored for antibacterial and antioxidant efficacies. However, there is limited information on its chemical composition and mechanism of action. The purpose of this study was to assess the chemical composition, antibacterial and antioxidant activities and mechanism of the whole leaf extract of A. arborescens Mill. The phytochemical profile was analysed with gas chromatography mass spectrometry (GC-MS). The antioxidant and antibacterial activities were screened using 1,1diphenyl2picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and micro-dilution assays, respectively. The effects of the extract on the bacterial respiratory chain dehydrogenase, membrane integrity and permeability were analysed using iodonitrotetrazolium chloride, 260 absorbing materials and relative electrical conductivity assays. GC-MS spectrum revealed 26 compounds with N,N’-trimethyleneurea (10.56%), xanthine (8.57%) and 4-hexyl-1-(7-ethoxycarbonylheptyl)bicyclo[4.4.0]deca-2,5,7-triene (7.10%), being the major components. The extract also exhibited antioxidant activity with median concentration (IC50) values of 0.65 mg/mL on DPPH and 0.052 mg/mL on ABTS. The extract exhibited minimum inhibitory concentration (MIC) values ranging from 0.07 to 1.13 mg/mL. The extract inhibited the bacterial growth by destructing the activity of the respiratory chain dehydrogenase, membrane integrity and permeability. Therefore, the leaf extract has the potential to serve as a source of antibacterial and antioxidant compounds.

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Section: Discussionmentioning

confidence: 99%

Chemical Profile, Antioxidant and Antibacterial Activities, Mechanisms of Action of the Leaf Extract of Aloe arborescens Mill

Maliehe

Nqotheni

Shandu

et al. 2023

Plants

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“…The plant environment is considered an untapped niche because only 1% of the total population of microbes residing in it has been explored so far, and the majority of them (70%) are identified as fungi 7 . Bioprospecting for endophytic actinomycetes in plants is of particular interest since the plants may possess a unique internal environment 8 that may support the production of compounds with novel chemical structures 6 .…”

Section: Introductionmentioning

confidence: 99%

Multifunctional in vitro, in silico and DFT analyses on antimicrobial BagremycinA biosynthesized by Micromonospora chokoriensis CR3 from Hieracium canadense

Tanvir,

Ijaz,

Sajid

et al. 2024

Sci Rep

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Among the actinomycetes in the rare genera, Micromonospora is of great interest since it has been shown to produce novel therapeutic compounds. Particular emphasis is now on its isolation from plants since its population from soil has been extensively explored. The strain CR3 was isolated as an endophyte from the roots of Hieracium canadense, and it was identified as Micromonospora chokoriensis through 16S gene sequencing and phylogenetic analysis. The in-vitro analysis of its extract revealed it to be active against the clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and Candida tropicalis (15 mm). No bioactivity was observed against Gram-negative bacteria, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 706003. The Micromonospora chokoriensis CR3 extract was also analyzed through the HPLC-DAD-UV–VIS resident database, and it gave a maximum match factor of 997.334 with the specialized metabolite BagremycinA (BagA). The in-silico analysis indicated that BagA strongly interacted with the active site residues of the sterol 14-α demethylase and thymidylate kinase enzymes, with the lowest binding energies of − 9.7 and − 8.3 kcal/mol, respectively. Furthermore, the normal mode analysis indicated that the interaction between these proteins and BagA was stable. The DFT quantum chemical properties depicted BagA to be reasonably reactive with a HOMO-LUMO gap of (ΔE) of 4.390 eV. BagA also passed the drug-likeness test with a synthetic accessibility score of 2.06, whereas Protox-II classified it as a class V toxicity compound with high LD50 of 2644 mg/kg. The current study reports an endophytic actinomycete, M. chokoriensis, associated with H. canadense producing the bioactive metabolite BagA with promising antimicrobial activity, which can be further modified and developed into a safe antimicrobial drug.

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Streptomyces Endophytes in Edible Plants: New Insights into their Chemistry and Health Benefits

Moussa

2024

Chemistry & Biodiversity

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Streptomyces is the largest source of microbial antibiotics with about 50% of marketed antimicrobial drugs originating from this genus. Endophytic streptomyces are the link between medicinal plants and the microbial world. Edible plants endophytic streptomyces were not targeted before despite their uniqueness and importance. In this review, we analyzed the chemical diversity of more than 150 compounds belonging to endophytic Streptomyces chemical classes such as alkaloids, polyketides, peptides, macrolides and terpenes and their biological activities. This analysis showed a dominant antimicrobial effect for most of the isolated compounds and highlighted an underestimated diversity to be studied or repurposed for other biological activities. Return to edible plants use and conducting toxicity studies to rationalize their nutraceutical potential based on their beneficial endophytes is urged. Although there are many studies for non‐vertebrates, the nutraceutical potential of these plants is expected to improve the gut microbiota since they are enriched with bioactive compounds from streptomyces species. This is the first review to discuss edible plants associated streptomyces, and we prospect that many studies will follow to unravel the mysterious health benefits of streptomyces in the human microbiome and encourage the revival of a correct lifestyle for the sack of a healthier microbiome.

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Bioprospecting of endophytic actinobacterium associated with Aloe ferox mill for antibacterial activity

Cited by 5 publications

References 55 publications

Chemical Profile, Antioxidant and Antibacterial Activities, Mechanisms of Action of the Leaf Extract of Aloe arborescens Mill

Chemical Profile, Antioxidant and Antibacterial Activities, Mechanisms of Action of the Leaf Extract of Aloe arborescens Mill

Multifunctional in vitro, in silico and DFT analyses on antimicrobial BagremycinA biosynthesized by Micromonospora chokoriensis CR3 from Hieracium canadense

Streptomyces Endophytes in Edible Plants: New Insights into their Chemistry and Health Benefits

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