Bioprospeksi Kapang yang Berasosiasi dengan Biota Laut Asal Kepulauan Seribu sebagai Antitumor T47D dan HepG2

Fajarningsih, Nurrahmi Dewi; Pratitis, Asri; Wikanta, Thamrin; Chasanah, Ekowati

doi:10.15578/jpbkp.v7i1.251

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…Bagian permukaan akar, daun, dan batang dihilangkan menggunakan cutter, kemudian dipotong kecil-kecil dan diletakkan pada media Zobell 2216E (Strobel dan Daisy,2003;Kumala dan Siswanto, 2007). Media Zobell adalah media yang umum digunakan untuk menumbuhkan bakteri laut (Radjasa et al, 2007;Fajarningsih et al, 2012). Simpan pada suhu ruang untuk menumbuhkan mikroba.…”

Section: Materi Dan Metodeunclassified

Potensi Bakteri Endofit Lamun Enhalus sp. dengan Aktivitas Antimikrofouling dari Perairan Lampung

2021

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Enhalus sp. adalah salah satu jenis lamun dan merupakan tanaman yang sangat penting bagi organisme dan mikroorganisme laut diperairan, salah satunya adalah bakteri karena lamun menyediakan tempat hidup serta penyedia nutrisi pada bakteri. Beberapa hasil penelitian, menyatakan bahwa ekstrak lamun memiliki aktivitas terhadap bakteri yang membentuk biofilm. Terjadinya proses Biofouling diawali dengan terbentuknya film oleh bakteri yang menempel pada permukaan benda yang terendam di dalam laut yang disebut sebagai biofilm. Salah satu cara meminimalisasi biofouling pada umumnya dengan menggunakan bahan beracun yang tidak ramah lingkungan, sehingga perlu dilakukan pencarian alternatif baru untuk menghambat bakteri biofilm. Upaya untuk mengatasi permasalahan dengan menggunakan isolat bakteri endofit lamun merupakan salah satu alternatif ramah lingkungan. Penelitian ini bertujuan untuk mendapatkan bakteri endofit lamun Enhalus sp. yang mampu menghambat pertumbuhan bakteri biofouling. Pada penelitian ini, sampel didapatkan dari pantai Ketapang Pesawaran dan pantai Biha, Pesisir Barat Lampung yang diambil dari bagian akar, batang dan daun lamun. Tahapan penelitian yang dilakukan yaitu pengambilan sampel, isolasi dan purifikasi bakteri endofit lamun dengan metode gores diatas agar, pembentukan bakteri biofilm sebagai bakteri uji pada uji antagonisme, isolasi bakteri biofilm, dan uji antagonis bakteri. Hasil dari isolasi dan purifikasi didapatkan 53 isolat bakteri endofit lamun Enhalus sp. yang akan diuji pada 25 isolat bakteri biofilm. Uji antagonis bakteri endofit lamun dengan kode LEDB2.4 dapat menghambat 4 jenis bakteri biofilm. Besar zona hambat terbesar yaitu terhadap bakteri biofilm dengan kode 3.1 sebesar 18,73 mm. Seagrass Enhalus sp. is a plant that very important for marine organisms and microorganisms in waters, one of which is bacteria because seagrass provides a place to live and nutrients for bacteria. Some research results state that seagrass extracts have activity against bacteria that form biofilms. The occurrence of the biofouling process begins with the formation of a film by bacteria attached to the surface of objects submerged in the sea which is called a biofilm. One way to minimize biofouling in general is to use toxic materials that are not environmentally friendly, so it is necessary to search for new alternatives to inhibit biofilm bacteria. Efforts to overcome the problem by using endophytic bacteria isolates from seagrass are an environmentally friendly alternative. This study aims to obtain the endophytic bacteria of seagrass Enhalus sp. which can inhibit the growth of biofouling bacteria. In this study, samples were obtained from Ketapang beach Pesawaran and Biha beach, West Coastal of Lampung that’s taken from the roots, stems and leaves of seagrass. The stages of the research carried out were sampling, isolation and purification of endophytic bacteria from seagrass, formation of biofilm bacteria as test bacteria in the antagonism test, and isolation of bacterial biofilm, and bacterial antagonist test. The results of isolation and purification obtained 53 isolates of seagrass endophytic bacteria which will be tested on 25 isolates of biofilm bacteria. Antagonistic test of seagrass endophytic bacteria with code LEDB2.4 can inhibit 4 types of biofilm bacteria. The largest inhibition zone was against biofilm bacteria with code 3.1 of 18.73 mm.

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Section: Materi Dan Metodeunclassified

Potensi Bakteri Endofit Lamun Enhalus sp. dengan Aktivitas Antimikrofouling dari Perairan Lampung

2021

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“…Several studies have been conducted about sponges in Seribu Islands National Park, ranging from diversity (De Voogd & Cleary, 2008), transplantation of sponges (Suparno et al, 2012), isolation of potential antitumor metabolites (Fajarningsih et al, 2006), isolation of protease inhibitor (Nurhayati et al, 2004), isolation of associated fungi from sponge (Fajarningsih et al, 2012). Study on bacterial diversity associated with Aaptos sp.…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Sponges Obtained From Seribu Islands National Park and Their Associated Bacteria

Patantis¹,

Rahmadara²,

Elfidasari³

et al. 2013

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Sponges are simple multicellular animals that produced many pharmaceutical secondary metabolites. Some sponge-associated bacteria are proven to produce the same metabolites as their host, giving an opportunity to mass produce the potential metabolites. The aim of this research was to analyze the diversity of sponge-associated bacteria and to identify the host sponge. Samples were collected from Seribu Islands National Park. Partial identification of sponges were conducted by molecular technique with the mitochondrial cytochrome oxidase subunit 1 (CO1) as the target area. The diversity of sponge-associated bacteria was determined by Terminal Restriction Fragment Length Polymorphism (T-RFLP) method. Result showed that sponges PS-17-12 has similarity with Petrosia sp., while PS-26-12 and PS-38-12 has similarity with Xestospongia muta. From the 3 sponge samples, 85 species of bacteria was obtained which can be classified into 9 phylums and 1 uncultured bacteria/environment sample. Some of the sponge-associated bacteria identified were known as a potential producer of metabolites with antibiotic activity.

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Overview of bioactivity studies on marine natural products

Zamani

Rahman

Rosada

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

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Marine natural products are sourced from marine biodiversity as natural raw materials for various commercial products. This study aims to review natural products of marine organisms and gap analysis for future research or challenges. A total of 109 references from 24 countries were collected. The analysis was carried out quantitatively and qualitatively. The bioactive compounds produced wereantioxidants, antibacterial, anticancer, antimicrobial, anti-fouling, antifungal, and anti-tumoral substances. Some marine organisms that can store chemical compounds through secondary metabolite processes are mangroves, seagrasses, macroalgae, microalgae, soft corals, molluscs, echinoderms, gastropods, cnidarians, sponges, fungi, and bacteria. Most of the papers only discuss the identification stage of the active compound, and some focus on product development. There are very few studies on prospects of commercialization and mass production. The problem to achieve mass production is due to the lack of interdisciplinary research collaboration. Future research challenges need to develop a transdisciplinary approach to study bioprospection research from upstream to downstream, starting from the potential identification of bioactive ingredients, product development, the availability of raw materials for mass production as well as commercialization and marketing.

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Bioprospeksi Kapang yang Berasosiasi dengan Biota Laut Asal Kepulauan Seribu sebagai Antitumor T47D dan HepG2

Cited by 4 publications

References 14 publications

Potensi Bakteri Endofit Lamun Enhalus sp. dengan Aktivitas Antimikrofouling dari Perairan Lampung

Potensi Bakteri Endofit Lamun Enhalus sp. dengan Aktivitas Antimikrofouling dari Perairan Lampung

Molecular Identification of Sponges Obtained From Seribu Islands National Park and Their Associated Bacteria

Overview of bioactivity studies on marine natural products

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