Bioreactive Compounds and Antioxidant Properties of Methanolic Extract of Fennel (Foeniculum Vulgare Miller)

Abstract

Methanolic extract of dried Fennel (Foeniculum vulgare Miller) seeds was analyzed for the presence of bioreactive compounds: ascorbate, riboflavin, tocopherol and polyphenols with potential antioxidant properties. The extract had low ascorbate (197.12+1.82µg), riboflavin (11.97+0.35µg) and tocopherol (280.33+5.67µg/g dry seeds) content. However the extract had high polyphenol content (16.506+0.32mg/g dry seeds). Antioxidant activity of the extract was determined by various mechanisms including DPPH free radical scavenging, metal induced protein and lipid oxidation inhibition and protection of DNA against H2O2 induced damage. Fennel had excellent free radical scavenging activity with IC50 2.1mg dry seed weight. IC50 observed for protection of proteins and lipids against metal ion induced oxidation is 2.1 and 2.5mg dry seed weight respectively. Extract equivalent to 0.5µg seeds is enough to protect DNA against H2O2 induced oxidation. The results suggest that polyphenols are the principal components responsible for high antioxidant potential of methanolic extract of fennel.

Antioxidant and antibacterial activities of different plants extracts against <i>Staphylococcus aureus</i> isolated from soccer player’s shoes and knowledge and applications about foot hygiene of the soccer players

“…Researchers reported that where they bring about their effect, causing either an increase in ion permeability and leakage of vital intracellular constituents, or impairment of the bacterial enzyme systems (Ratledge and Wilkinson, 1988;Wendakoon and Sakaguchi, 1995). Dua et al (2013) reported that antibacterial activities of Foeniculum vulgare Miller seeds were found as 11 mm inhibition zone against S. aureus (Dua et al, 2013). This report also supports the results we obtained from our study.…”

Antioxidant Profile of Coriandrum Sativum Methanolic Extract

“…After incubating at 37°C for 6 h the contents were chilled and 5 ml of 85 % H 2 SO 4 was added slowly. The absorbance was read at 540 nm against reagent blank after 30 minutes 10 . A calibration curve of ascorbic acid (1-20 µg/ml) was prepared.…”

“…Within 2 minutes, 3 ml of H 2 O 2 -water solution (1:1) was added and pH adjusted to 7.0 with NaOH. The fluorescence by the filtrate was measured at 530 nm with excitation at 470 nm using fluorescence spectrophotometer 10 . Standard riboflavin (1 µg/ml) was used for calibration purpose.…”

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“…Traditionally, fennel seeds are used as anti-inflammatory, analgesic, carminative, diuretic and antispasmodic agents. Recently there has been considerable interest in the antioxidant potential and antimicrobial activities of fennel seed extracts and essential oil [2][3] . Cardamom, is one of the most widely known spices due to its strong aroma and flavor.…”