Bioreactor for mammalian cell culture

Prokop, A.; Rosenberg, Morris Z.

doi:10.1007/bfb0051951

Cited by 32 publications

(18 citation statements)

References 90 publications

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“…The cell's viability, adhesion, and proliferation on the scaffold are used to indicate the biocompatibility and appropriateness of medical application. Because proliferation of most mammalian cell types depends on anchorage, the implanted scaffold must provide a suitable ability for cell adhesion, proliferation, and migration [28,29]. In this study, S and SA performed as a great potential biomaterial for mammalian cell migration, cell attachment, and long-term viability supporting, while A scaffold was not due to its structure and properties.…”

Section: Animal Studymentioning

confidence: 98%

“…In A scaffold, a polysaccharide structure, the most intense band observed at 1032 cm −1 is common to all the polysaccharide molecules and had contributions from several vibration modes, such as C-O, C-O-H, and C-C stretching [17]. The strong band at 929 cm −1 assigned to the presence of 3,6-anhydrogalactose residue was common to agar [18]. The feature bands of agars and silk protein are all observed in SA spectra.…”

Section: Ft-ir Analysismentioning

confidence: 99%

“…The previous studies show that agar has good biocompatibility and can be considered as a potential adhesive factor for biomaterial structure but it does not provide sufficient support for cell adhesion [26,27], while BMSF has the vigorous ability to support cell attachment and growth but it is hard to keep the fiber mesh in the humid condition [28,29]. So we attempt to combine two ingredients, agar and BMSF, to improve shortcomings of those biomaterials.…”

Section: Animal Studymentioning

confidence: 99%

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Evaluation of the Morphology and Biocompatibility of Natural Silk Fibers/Agar Blend Scaffolds for Tissue Regeneration

Luong

Thanh-Truc

Toi

et al. 2018

International Journal of Polymer Science

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This study was aimed to develop a tissue engineering scaffold by incorporation of Bombyx mori silk fiber (BMSF) and agar. This promised the improvement in enhancing their advantageous properties as well as limiting their defects without occurring chemical reactions or crosslink formation. The morphology and chemical structure of scaffolds were observed using scanning electron microscope (SEM) observation and Fourier transform infrared (FT-IR) spectra. The SEM results show that scaffolds containing BMSF have microporous structures, which are suitable for cell adhesion. Agar scaffolds, by contrast, had much more flat morphology. FT-IR spectra confirm that no modifications to BMSF happened in scaffolds, which indicates that there was no chemical reaction or crosslink formation between silk and agar in this process. Furthermore, the biocompatibility of scaffolds was performed in the mouse's subcutaneous part of the dorsal region for 15 days, followed by Haematoxylin and Eosin (H&E) staining. H&E staining results demonstrate that scaffolds had good biocompatibility and there was no sign of the body rejection in all of samples. The results from animal study show that SA scaffolds have the most stable structure for cell adhesion compared with those single materials.

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Section: Animal Studymentioning

confidence: 98%

Section: Ft-ir Analysismentioning

confidence: 99%

Section: Animal Studymentioning

confidence: 99%

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Evaluation of the Morphology and Biocompatibility of Natural Silk Fibers/Agar Blend Scaffolds for Tissue Regeneration

Luong

Thanh-Truc

Toi

et al. 2018

International Journal of Polymer Science

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“…Adequate oxygen mass transfer and acceptable levels of shear stress are critical characteristics to maintain when scaling up suspension cultures of mammalian cells (Bliem et al, 1991;Kawase and MooYoung, 1988;Marks, 2003;Prokop and Rosenberg, 1989). These characteristics are influenced by vessel geometry, impeller design, and addition of equipment such as sparging devices and measurement probes, as well as the cell culture medium properties.…”

Section: Important Design Considerations For Scale-up Of Suspension Cmentioning

confidence: 97%

Scaled‐up production of mammalian neural precursor cell aggregates in computer‐controlled suspension bioreactors

Gilbertson

Sen

Behie

et al. 2006

Biotech & Bioengineering

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The clinical use of neural precursor cells (NPCs) for the treatment of neurological diseases, such as Parkinson's disease and Huntington's disease, requires overcoming the scarcity of these cells through controlled expansion. The main objective of the present study was to develop a large-scale computer-controlled bioprocess for the expansion of mammalian NPCs in suspension culture by scaling up existing reactor protocols. In order to support the oxygen demands of the maximum cell densities achieved, the volumetric mass transfer coefficient was kept above 1.10/h while scaling-up from small-scale 125 mL vessels to large-scale 500 mL bioreactors. In addition, the maximum shear stress at the impeller tip was maintained between 0.30 and 0.75 Pa to reduce damage to the cells. The resulting large-scale bioprocess achieved maximum viable cell densities of 1.2 x 10(6) cells/mL and a batch multiplication ratio of 9.1. Moreover, the process successfully maintained the NPC characteristics observed in small-scale studies.

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“…Many of these have been scaled back to the 0.250-10 L scale and presently several excellent laboratory or pilot scale bioreactors are commercially available (for reviews see Hu and Piret, 1992;Kumar et al, 2004;Prokop and Rosenberg, 1989). While these systems offer advantages such as real-time control of DO, pH, and a wide range of metabolites (glucose, lactate, pyruvate, acetate, and amino acids among them), they come with a significant cost.…”

Section: Culture Conditionsmentioning

confidence: 99%

Cost‐effective engineering of a small‐scale bioreactor

Bartholomew

Tansey²

2006

Biotech & Bioengineering

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Several methods exist for increasing the scale of cell culture in the laboratory. While these methods provide significant increases in biomass, they are often prohibitively expensive for many laboratories. We have engineered a small-scale bioreactor with a novel means of introducing oxygen through the catalytic breakdown of hydrogen peroxide using a manganese oxide catalyst. We have also adapted and modified an existing assay for dissolved oxygen to be compatible with culture conditions. In this system we have been able to culture CHO cells at densities of up to 10(7) cells/mL without the use of automated feedback systems.

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Bioreactor for mammalian cell culture

Cited by 32 publications

References 90 publications

Evaluation of the Morphology and Biocompatibility of Natural Silk Fibers/Agar Blend Scaffolds for Tissue Regeneration

Evaluation of the Morphology and Biocompatibility of Natural Silk Fibers/Agar Blend Scaffolds for Tissue Regeneration

Scaled‐up production of mammalian neural precursor cell aggregates in computer‐controlled suspension bioreactors

Cost‐effective engineering of a small‐scale bioreactor

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