Bioreactor scale-up and oxygen transfer rate in microbial processes: An overview

Garcı́a-Ochoa, Félix; Gómez, Eduardo Escalante

doi:10.1016/j.biotechadv.2008.10.006

Cited by 1,177 publications

(825 citation statements)

References 147 publications

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“…Nowadays, bubble columns are studied as an efficient photo-bioreactor for production of microalgae [5][6][7]. The supply of gas to the liquid is a crucial process to keep microorganisms alive and the knowledge of gas-liquid mass transfer rate is an important design and scale-up parameter determining the reactor efficiency [2,8].…”

Section: Introductionmentioning

confidence: 99%

“…The mass transfer rate is characterized by the volumetric mass transfer coefficient, k L a, and there exists plenty of papers providing correlations predicting this parameter in bubble columns (reviewed e.g., in [2,3,8]). However, the comparison of these predictions brings substantial data scatter and the choice of the proper correlation becomes difficult.…”

Section: Introductionmentioning

confidence: 99%

“…Data differences can be attributed to the different hydrodynamic conditions in the bubble column generated by different types of gas distributors and also to the various methods used for k L a measurements. As different measurement methods can be affected by different assumptions and simplifications, various errors in k L a results can occur [8,[19][20][21]. In all the articles presented in Table 1, dynamic methods were used where physical absorption (without chemical reaction) of oxygen into a liquid occurs and the change of oxygen concentration is recorded by a probe and fitted by a theoretical model with k L a as the optimized parameter.…”

Section: Introductionmentioning

confidence: 99%

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Measurement of Volumetric Mass Transfer Coefficient in Bubble Columns

et al. 2018

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Abstract:The paper presents a brief overview of experiments on volumetric mass transfer coefficient in bubble columns. The available experimental data published are often incomparable due to the different type of gas distributor and different operating conditions used by various authors. Moreover, the value of the coefficient obtained experimentally is very sensitive to the particular method and to the physical models used in its evaluation. It follows that the Dynamic Pressure Method (DPM) is able to provide physically correct values not only in lab-scale contactors but also in pilot-scale reactors. However, the method was not correctly proven in bubble columns. In present experiments, DPM was employed in a laboratory-scale bubble column with a coalescent phase and tested in the pure heterogeneous flow regime. The method was successfully validated by the measurements under two different conditions relevant to the mass transfer. First, the ideal pressure step was compared with the non-ideal pressure step. Second, the pure oxygen absorption was compared with the simultaneous oxygen-and-nitrogen absorption. The obtained results proved that DPM is suitable for measuring the mass transport in bubble columns and to provide reliable data of volumetric mass transfer coefficient.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Measurement of Volumetric Mass Transfer Coefficient in Bubble Columns

et al. 2018

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“…Gas to liquid transfer is influenced by the viscosity and volume of the medium, stirrer design/speed and flow rates [67]. The fragile nature of adult nematodes requires low impeller velocities to prevent disruption; therefore, a compromise between agitation and oxygen flow must be reached.…”

Section: Oxygen Availability and Agitationmentioning

confidence: 99%

“…The fragile nature of adult nematodes requires low impeller velocities to prevent disruption; therefore, a compromise between agitation and oxygen flow must be reached. Oxygenation is crucial because bacterial demands will compete with those of the nematodes; therefore, an oxygen flow of 1 volume of gas to 1 volume of media per min (vvm) is recommended to prevent the build-up of CO 2 [67]. When culturing EPNs, the dissolved oxygen should be high, approximately 40 %, for juveniles and approaching 60 % for adults and the agitation should not be greater than 60 rpm [37].…”

Section: Oxygen Availability and Agitationmentioning

confidence: 99%

Mass Production of the Beneficial Nematode Heterorhabditis bacteriophora and Its Bacterial Symbiont Photorhabdus luminescens

2012

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Entomoparasitic nematodes (EPNs) are being commercialized as a biocontrol measure for crop insect pests, as they provide advantages over common chemical insecticides. Mass production of these nematodes in liquid media has become a major challenge for commercialization. Producers are not willing to share the trade secrets of mass production and by doing so, have made culturing EPNs extremely difficult to advance existing technologies. Theoretically, mass production in liquid media is an ideal culturing method as it increases cost efficiency and nematode quantity. This paper will review current culturing methodologies and suggest basic culturing parameters for mass production. This review is focused on Heterorhabditis bacteriophora; however, this information can be useful for other nematode species.

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Bioreactor Scale‐Up

Bowers

Seamans

et al. 2018

Kirk-Othmer Encyclopedia of Chemical Technology

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Large‐scale bioreactors are used for producing biological products (e.g., monoclonal antibodies, fusion proteins, enzymes) expressed in mammalian cells. Compared with small‐scale bioreactors, they are intrinsically more challenging in mixing and mass transfer and require more optimization. Proper bioreactor scale‐up is needed to ensure productivity and product quality. Here, common approaches and challenges in industrial mammalian cell culture scale‐up are covered. These are different from the scale‐up challenges and methodologies typically used in microbial bioreactors, since cell‐culture bioreactors operate in different agitation power and gas flow regimes. Engineering considerations including mass transfer, mixing, and hydrodynamic stress are presented in detail. Single‐use bioreactors are focused due to their rapid adoption by industry in clinical and commercial production. Though the main body of this article is on suspension cultures, special considerations in scaling‐up microcarrier‐based cultures are also briefly discussed. Finally, new challenges and desired improvements in scaling up high density cultures are presented.

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Bioreactor scale-up and oxygen transfer rate in microbial processes: An overview

Cited by 1,177 publications

References 147 publications

Measurement of Volumetric Mass Transfer Coefficient in Bubble Columns

Measurement of Volumetric Mass Transfer Coefficient in Bubble Columns

Mass Production of the Beneficial Nematode Heterorhabditis bacteriophora and Its Bacterial Symbiont Photorhabdus luminescens

Bioreactor Scale‐Up

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