Bioreactors for Guiding Muscle Tissue Growth and Development

Dennis, Robert G.; Smith, Brian J.; Philp, Andrew; Donnelly, Kenneth; Baar, Keith

doi:10.1007/978-3-540-69357-4_3

Cited by 27 publications

(20 citation statements)

References 267 publications

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“…The reversal of the fast-to-slow fiber type switch could be achieved by removing stimulation, denervation, administration of tetrodotoxin, stimulation at high frequency, and shortening of the muscle length (109). Still, slow-to-fast fiber type transition is more difficult to achieve due to lower work-to-rest ratio and random firing pattern of the fast muscle fibers (110). Lately, Guo et al developed a functional human neuromuscular junction system by co-culture of human stem cell derived motoneurons and primary muscle cells (111).…”

Section: Use Of Biophysical Stimulation To Promote Engineered Muscle mentioning

confidence: 99%

“…Overall, multimodal bioreactors hold promise in providing the most biomimetic culture environment for growth and maturation of engineered striated muscles. However, with increased bioreactor complexity, susceptibility to failure increases and so do the difficulties in identifying the optimal regime of stimulation (110). In addition, translational potential of the entire approach may decrease due to potential regulatory and commercialization issues.…”

Section: Use Of Biophysical Stimulation To Promote Engineered Muscle mentioning

confidence: 99%

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Use of Flow, Electrical, and Mechanical Stimulation to Promote Engineering of Striated Muscles

2013

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The field of tissue engineering involves design of high-fidelity tissue substitutes for predictive experimental assays in vitro and cell-based regenerative therapies in vivo. Design of striated muscle tissues, such as cardiac and skeletal muscle, has been particularly challenging due to a high metabolic demand and complex cellular organization and electromechanical function of the native tissues. Successful engineering of highly functional striated muscles may thus require creation of biomimetic culture conditions involving medium perfusion, electrical and mechanical stimulation. When optimized, these external cues are expected to synergistically and dynamically activate important intracellular signaling pathways leading to accelerated muscle growth and development. This review will discuss the use of different types of tissue culture bioreactors aimed at providing conditions for enhanced structural and functional maturation of engineered striated muscles.

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Section: Use Of Biophysical Stimulation To Promote Engineered Muscle mentioning

confidence: 99%

Section: Use Of Biophysical Stimulation To Promote Engineered Muscle mentioning

confidence: 99%

Use of Flow, Electrical, and Mechanical Stimulation to Promote Engineering of Striated Muscles

2013

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“…Functional hydrogel-based skeletal muscle tissues with dense, aligned and highly differentiated muscle fibers have been engineered in several different bioreactors for various different applications (23–33). Recent work addressed methods to control the final 3D tissue architecture using microfabrication technology (33–35), but still little quantitative data are available on the mechanical and chemical factors affecting the structure and function of individual cells inside the tissue.…”

Section: Introductionmentioning

confidence: 99%

Formation and optogenetic control of engineered 3D skeletal muscle bioactuators

et al. 2012

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Densely arrayed skeletal myotubes are activated individually and as a group using precise optical stimulation with high spatiotemporal resolution. Skeletal muscle myoblasts are genetically encoded to express light-activated cation channel, Channelrhodopsin-2, which allows for spatiotemporal coordination of the multitude of skeletal myotubes that contract in response to pulsed blue light. Furthermore, ensembles of mature functional 3D muscle microtissues have been formed from the optogenetically encoded myoblasts using a high-throughput device. The device, called “skeletal muscle on a chip”, not only provides the myoblasts with controlled stress and constraints necessary for muscle alignment, fusion and maturation, but also facilitates to measure forces and characterize the muscle tissue. We measured the specific static and dynamic stresses generated by the microtissues, and characterized the morphology and alignment of the myotubes within the constructs. The device allows for testing the effect of a wide range of parameters (cell source, matrix composition, microtissue geometry, auxotonic load, growth factors, and exercise) on the maturation, structure, and function of the engineered muscle tissues in a combinatorial manner. Our studies integrate tools from optogenetics and microelectromechanical systems (MEMS) technology with skeletal muscle tissue engineering to open up opportunities to generate soft robots actuated by multitude of spatiotemporally coordinated 3D skeletal muscle microtissues.

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“…Without major advances, especially in the area of vascularization, use of engineered muscle as a graft material will be severely limited. Implantation of engineered skeletal muscle into an in vivo regenerative environment, however, has promoted development towards the adult phenotype(Corona et al 2014; VanDusen et al 2014), and several recent studies have attempted to utilize key chemical and mechanical stimuli to improve the maturity of these engineered muscles in vitro (Dennis et al 2009; Martin et al 2013). This review focuses on important in vitro biochemical stimuli, summarizing the current state of the art in growth factors utilized for skeletal muscle tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Growth Factors for Skeletal Muscle Tissue Engineering

Syverud¹,

VanDusen

Larkin³

2016

Cells Tissues Organs

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Tissue-engineered skeletal muscle holds promise as a source of graft tissue for repair of volumetric muscle loss and as a model system for pharmaceutical testing. To reach this potential, engineered tissues must advance past the neonatal phenotype that characterizes the current state of the art. In this review, we describe native skeletal muscle development and identify important growth factors controlling this process. By comparing in vivo myogenesis to in vitro satellite cell cultures and tissue engineering approaches, several key similarities and differences that may potentially advance tissue-engineered skeletal muscle were identified. In particular, hepatocyte and fibroblast growth factors used to accelerate satellite cell activation and proliferation, followed by addition of insulin-like growth factor as a potent inducer of differentiation, are proven methods for increased myogenesis in engineered muscle. Additionally, we review our recent novel application of dexamethasone (DEX), a glucocorticoid that stimulates myoblast differentiation, in skeletal muscle tissue engineering. Using our established skeletal muscle unit (SMU) fabrication protocol, timing- and dose-dependent effects of DEX were measured. The supplemented SMUs demonstrated advanced sarcomeric structure and significantly increased myotube diameter and myotube fusion compared to untreated controls. Most significantly, these SMUs exhibited a fivefold rise in force production. Thus, we concluded that DEX may serve to improve myogenesis, advance muscle structure, and increase force production in engineered skeletal muscle.

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Bioreactors for Guiding Muscle Tissue Growth and Development

Cited by 27 publications

References 267 publications

Use of Flow, Electrical, and Mechanical Stimulation to Promote Engineering of Striated Muscles

Use of Flow, Electrical, and Mechanical Stimulation to Promote Engineering of Striated Muscles

Formation and optogenetic control of engineered 3D skeletal muscle bioactuators

Growth Factors for Skeletal Muscle Tissue Engineering

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