Bioreactors for Plant Embryogenesis and Beyond

Fei, Liwen; Weathers, Pamela J.

doi:10.1007/978-1-4939-3061-6_10

Cited by 10 publications

(9 citation statements)

References 77 publications

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“…The induction frequency on the medium supplemented with PGRs varied from 1.0 to 2.8%, with an overall average of 2.3% (Maruyama et al, 2005a,b, 2007; Hosoi and Maruyama, 2012), which was comparable with previous results for P. densiflora (up to 2.3%, Kim and Moon, 2014), P. nigra (3.1%, Salajová and Salaj, 2005), Pinus banksiana (up to 3.9%, Park et al, 2006), P. rigida × P. taeda (up to 1.1%, Kim and Moon, 2007b), and P. lambertiana (1–3%, Gupta, 1995). These results were inconsistent with the high induction rates reported for P. radiata (44–93%, Hargreaves et al, 2011), P. taeda (up to 79%, Gupta, 2014), P. strobus (54%, Finer et al, 1989), Pinus sylvestris (up to 30%, Aronen et al, 2009), and P. pinaster (up to 75%, Park et al, 2006).…”

Section: Induction and Proliferation Of Etmentioning

confidence: 65%

“…GG is the most frequently used gelling agent to solidify a medium. 2,4-Dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BA) are the most common PGRs used for SE initiation and proliferation of ET; occasionally, for SE initiation, naphthaleneacetic acid (NAA) is used instead of 2,4-D (Gupta, 2014), and N -(2-chloro-4-pyridyl)- N ’-phenylurea (CPPU) is used as sole PGR in the medium (Park et al, 2006). Supplement of other PGRs, such brassinolides, triacontanol, and salicylic acid, can improve SE initiation rates (Malabadi et al, 2011).…”

Section: Induction and Proliferation Of Etmentioning

confidence: 99%

“…Improved protocols already used for P. radiata (Hargreaves et al, 2011), efficiently improved the average induction rate of ET in Douglas-fir; such protocols include disinfection of whole cones (instead of seeds), modification of induction medium (GlitzB medium), and culture of zygotic embryos (instead of whole megagametophytes) (Reeves et al, 2018). Gupta (2014) reported a notable improvement of 3–4 times of the average induction rate of ET in loblolly pine by culturing transversally dissected megagametophytes (two parts, instead of whole explant) in solid initiation medium and post-addition of liquid medium containing 10 mgl -1 ABA after 2 weeks of culture.…”

Section: Induction and Proliferation Of Etmentioning

confidence: 99%

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Progress in Somatic Embryogenesis of Japanese Pines

Maruyama

Hosoi

2019

Front. Plant Sci.

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Somatic embryogenesis (SE) in not only one of the most promising techniques for mass propagation of selected trees, but also is a valuable tool for basic research studies in cell biology and genetic engineering, and it allows the long-term ex situ conservation of genetic resources by cryopreservation techniques. This review reports the most recent progress in SE, protoplast culture, and cryopreservation of four important Japanese pines (Pinus thunbergii, Pinus densiflora, Pinus armandii var. amamiana, and Pinus luchuensis). Induction of embryogenic tissues (ET), embryogenic culture maintenance/proliferation, production of somatic embryos, germination, and conversion to plants are described focusing on the protocols most commonly reported for plant production in Pinus species through to SE.

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Section: Induction and Proliferation Of Etmentioning

confidence: 65%

Section: Induction and Proliferation Of Etmentioning

confidence: 99%

Section: Induction and Proliferation Of Etmentioning

confidence: 99%

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Progress in Somatic Embryogenesis of Japanese Pines

Maruyama

Hosoi

2019

Front. Plant Sci.

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“…Brief information about the bioreactors most frequently used for the propagation of trees is given below. For detailed information on these topics, readers are referred to several comprehensive reviews [21,[24][25][26][27][32][33][34][35].…”

Section: Bioreactors Systems For the Propagation Of Treesmentioning

confidence: 99%

Use of bioreactor systems in the propagation of forest trees

Vidal

Sánchez

2019

Engineering in Life Sciences

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Plant biotechnology can be used to conserve the germplasm of natural forests, and to increase the productivity and sustainability of plantations. Both goals imply working with mature trees, which are often recalcitrant to micropropagation. Conventional in vitro culture uses closed containers and gelled medium with sugar supplementation. Bioreactor culture uses liquid medium and usually incorporates aeration. The increased absorption of nutrients via the liquid medium together with the renewal of the air inside the bioreactors may improve the physiological state of the explants. In this review, we will explore the feasibility of using bioreactors to overcome the recalcitrance of many trees to micropropagation and/or to decrease the cost of large‐scale propagation. We will focus on the recent use of bioreactors during the multiplication, rooting (plant conversion in the case of somatic embryos), and acclimation stages of the micropropagation of axillary shoots and somatic embryos of forest trees (including some shrubs of commercial interest), in both temporary and continuous immersion systems. We will discuss the advantages and the main obstacles limiting the widespread implementation of bioreactor systems in woody plant culture, considering published scientific reports and contributions from the business sector.

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“…Somatic embryogenesis has also been revealed as the best way of regeneration in cryopreservation (Engelmann, 2004) and genetic transformation procedures (Giri et al., 2004), and it is increasingly important in functional genomics studies, in order to validate genes related to the embryogenic process. The application of bioreactors with continuous or temporal immersion systems (TIS) for culture of somatic embryos helps to improve the quality of the embryos, to increase the proliferation and conversion rates, and at the same time to reduce the production costs (Fei and Weathers, 2015). Somatic embryogenesis also facilitates the production of synthetic seeds by applying encapsulation techniques to the somatic embryos (Guan et al., 2016).…”

Section: Why Somatic Embryogenesis On Holm Oak?mentioning

confidence: 99%

Holm Oak Somatic Embryogenesis: Current Status and Future Perspectives

et al. 2019

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Quercus ilex (holm oak) is one of the most representative trees in the Mediterranean basin, but now the sustainability of its ecosystems is at serious risk due to the lack of natural regeneration and to the presence of a severe disease called oak decline that has caused the death of thousands of trees. The application of biotechnological tools, such as somatic embryogenesis, allows programs of genetic improvement of the species to be speeded up and helps in the conservation of its ecosystems. Somatic embryogenesis is currently considered one of the main biotechnological techniques that has demonstrated significant benefits when has applied to forest tree species, providing significant advantages such as mass propagation, genetic transformation, application of synthetic seed, and cryopreservation of elite genotypes. In this report, the state of the art of somatic embryogenesis in holm oak is reviewed. Factors affecting the induction (plant donor age, type of explant, or plant growth regulators) and maintenance and proliferation of the embryogenic cultures are summarized. Advances on the conversion of somatic embryos into plants and on the acclimatization of these plantlets, as well as the results obtained on the application of the genetic transformation and the cryopreservation procedures to holm oak embryogenic cultures, are also presented.

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Bioreactors for Plant Embryogenesis and Beyond

Cited by 10 publications

References 77 publications

Progress in Somatic Embryogenesis of Japanese Pines

Progress in Somatic Embryogenesis of Japanese Pines

Use of bioreactor systems in the propagation of forest trees

Holm Oak Somatic Embryogenesis: Current Status and Future Perspectives

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