Bioreactors for Tissue Engineering: Focus on Mechanical Constraints. A Comparative Review

Bilodeau, Katia

doi:10.1089/ten.2006.12.ft-163

Cited by 34 publications

(51 citation statements)

References 75 publications

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“…Bioreactors have been developed in order to address the need to circumvent the limitations of mass transfer in thick scaffold cultures, and in the case of bone tissue engineering, to apply suitable mechano-transduction forces which in turn facilitate osteogenic differentiation through specific signaling pathways [19,22]. In this study, we demonstrated that biaxial bioreactor matured hfMSC/PCL-TCP scaffolds resulted in significantly higher cellular proliferation, homogenous cellular distribution and in-vitro and invivo osteogenic differentiation than those cultured in static condition.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, shear stresses found within the biaxial bioreactor can provide key mechanical stimulation which has been implicated in the promotion of cellular proliferation of osteoprogenitor cell types [22,42].…”

Section: Discussionmentioning

confidence: 99%

“…Bioreactors enable improved flow perfusion of cellular scaffolds, which in turn improves mass transfer efficiencies. In addition, they provide a mechanical stimulation which triggers the mechano-transduction signaling pathway important for osteogenic differentiation in MSC cell types [19,21,22].…”

Section: Introductionmentioning

confidence: 99%

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A biaxial rotating bioreactor for the culture of fetal mesenchymal stem cells for bone tissue engineering

et al. 2009

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A biaxial rotating bioreactor for the culture of fetal mesenchymal stem cells for bone tissue engineering

et al. 2009

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“…In fact, cells in the human body are constantly subjected to chemical and mechanical stimuli, which ensure cell functionality and contribute to tissue organization. 2,3 Therefore, the use of bioreactors is becoming a fundamental step for the fabrication of three-dimensional (3D) cell/scaffold constructs for tissue engineering applications. Different bioreactor systems have been designed and used for the cultivation of bone-engineered substitutes, including spinner flasks, and rotating vessels, perfusion and compression systems, each providing specific mechanical stresses and regimes for the proper stimulation of the cell/scaffold constructs (for a review see Ref.…”

Section: Introductionmentioning

confidence: 99%

Human Embryonic Stem Cell-Derived Mesodermal Progenitors Display Substantially Increased Tissue Formation Compared to Human Mesenchymal Stem Cells Under Dynamic Culture Conditions in a Packed Bed/Column Bioreactor

Peppo

Sladkova

Sjövall

et al. 2013

Tissue Engineering Part A

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Bone tissue engineering represents a promising strategy to obviate bone deficiencies, allowing the ex vivo construction of bone substitutes with unprecedented potential in the clinical practice. Considering that in the human body cells are constantly stimulated by chemical and mechanical stimuli, the use of bioreactor is emerging as an essential factor for providing the proper environment for the reproducible and large-scale production of the engineered substitutes. Human mesenchymal stem cells (hMSCs) are experimentally relevant cells but, regardless the encouraging results reported after culture under dynamic conditions in bioreactors, show important limitations for tissue engineering applications, especially considering their limited proliferative potential, loss of functionality following protracted expansion, and decline in cellular fitness associated with aging. On the other hand, we previously demonstrated that human embryonic stem cell-derived mesodermal progenitors (hES-MPs) hold great potential to provide a homogenous and unlimited source of cells for bone engineering applications. Based on prior scientific evidence using different types of stem cells, in the present study we hypothesized that dynamic culture of hES-MPs in a packed bed/column bioreactor had the potential to affect proliferation, expression of genes involved in osteogenic differentiation, and matrix mineralization, therefore resulting in increased bone-like tissue formation. The reported findings suggest that hES-MPs constitute a suitable alternative cell source to hMSCs and hold great potential for the construction of bone substitutes for tissue engineering applications in clinical settings.

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“…This involves electromechanical conditioning [175,176], the development of micro-perfusion systems [23, [177][178][179][180][181][182][183], and neovascularization of 3D tissue constructs [184,185]. Although these variables are being independently developed, the goal would be to bring the pieces together to develop a controlled in vitro environment to promote tissue development and maturation.…”

Section: Discussionmentioning

confidence: 99%

Getting to the Heart of Tissue Engineering

Khait

Hecker

Blan

et al. 2008

J. of Cardiovasc. Trans. Res.

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Cardiovascular disease affects 80 million people in the USA and is the leading cause of death. Significant limitations of current treatments necessitate the development of novel strategies. Cardiovascular tissue engineering is an emerging field focused on the development of biological substitutes to restore, maintain, or improve tissue function. In this article, we present an overview of trends in the field and scientific milestones achieved during the last decade. Various 3D bioengineered models of functional cardiovascular structures, including cell-based cardiac pumps, ventricles, patches, vessels, and valves, are described. We discuss critical technological hurdles that must be addressed for continued progress and an outlook for the future of cardiovascular tissue engineering.

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Bioreactors for Tissue Engineering: Focus on Mechanical Constraints. A Comparative Review

Cited by 34 publications

References 75 publications

A biaxial rotating bioreactor for the culture of fetal mesenchymal stem cells for bone tissue engineering

A biaxial rotating bioreactor for the culture of fetal mesenchymal stem cells for bone tissue engineering

Human Embryonic Stem Cell-Derived Mesodermal Progenitors Display Substantially Increased Tissue Formation Compared to Human Mesenchymal Stem Cells Under Dynamic Culture Conditions in a Packed Bed/Column Bioreactor

Getting to the Heart of Tissue Engineering

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