Getting to the Heart of Tissue Engineering

Khait, Luda; Hecker, Louise; Blan, Nicole R.; Coyan, Garrett N.; Migneco, Francesco; Huang, Yen‐Chang; Birla, Ravi K.

doi:10.1007/s12265-007-9005-x

Cited by 13 publications

(5 citation statements)

References 162 publications

(132 reference statements)

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“…Of interest, the genetic manipulation of MSC to overexpress cytokines and growth factors, such as HGF, VEGF and SDF-1, have also been proposed to improve neo-angiogenesis and the endogenous mechanisms of tissue repair/regeneration [44,141,142]. Finally, bioengineered three-dimensional matrices have been shown to represent appropriate devices to preserve the survival of the engrafted MSCs and assist their migration within the damaged tissues [139,143,144]. …”

Section: Bone Marrow-derived Mesenchymal Stromal Cells (Bm-mscs)mentioning

confidence: 99%

Trophic Actions of Bone Marrow-Derived Mesenchymal Stromal Cells for Muscle Repair/Regeneration

Sassoli

Zecchi‐Orlandini

Formigli

2012

Cells

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Bone marrow-derived mesenchymal stromal cells (BM-MSCs) represent the leading candidate cell in tissue engineering and regenerative medicine. These cells can be easily isolated, expanded in vitro and are capable of providing significant functional benefits after implantation in the damaged muscle tissues. Despite their plasticity, the participation of BM-MSCs to new muscle fiber formation is controversial; in fact, emerging evidence indicates that their therapeutic effects occur without signs of long-term tissue engraftment and involve the paracrine secretion of cytokines and growth factors with multiple effects on the injured tissue, including modulation of inflammation and immune reaction, positive extracellular matrix (ECM) remodeling, angiogenesis and protection from apoptosis. Recently, a new role for BM-MSCs in the stimulation of muscle progenitor cells proliferation has been demonstrated, suggesting the potential ability of these cells to influence the fate of local stem cells and augment the endogenous mechanisms of repair/regeneration in the damaged tissues.

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Section: Bone Marrow-derived Mesenchymal Stromal Cells (Bm-mscs)mentioning

confidence: 99%

Trophic Actions of Bone Marrow-Derived Mesenchymal Stromal Cells for Muscle Repair/Regeneration

Sassoli

Zecchi‐Orlandini

Formigli

2012

Cells

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“…5 Tissue engineering strategies have evolved in response to this need and are focused on the fabrication of artificial tissue constructs in a controlled laboratory environment. [6][7][8][9][10] Research in the area of heart muscle tissue engineering is focused on the development of functional three-dimensional artificial heart muscle (3D-AHM), and more recently, organ fabrication technologies have been developed to support the fabrication of complete artificial hearts. [11][12][13] The most common strategy to fabricate 3D-AHM has been to culture neonatal cardiac myocytes within a suitable 3D scaffold, resulting in artificial tissue that replicates a partial subset of mammalian heart muscle functions.…”

mentioning

confidence: 99%

Establishing the Framework for Fabrication of a Bioartificial Heart

et al. 2015

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There is a chronic shortage of donor hearts. The ability to fabricate complete bioartificial hearts (BAHs) may be an alternative solution. The current study describes a method to support the fabrication and culture of BAHs. Rat hearts were isolated and subjected to a detergent based decellularization protocol to remove all cellular components, leaving behind an intact extracellular matrix. Primary cardiac cells were isolated from neonatal rat hearts, and direct cell transplantation was used to populate the acellular scaffolds. Bioartificial hearts were maintained in a custom fabrication gravity fed perfusion culture system to support media delivery. The functional performance of BAHs was assessed based on left ventricle pressure and on electrocardiogram. Furthermore, BAHs were sectioned and stained for the whole heart cardiac tissue distribution and for cardiac molecules, such as α-actinin, cardiac troponin I, collagen type I, connexin 43, von Willebrand factor, and ki67. Bioartificial hearts replicated a partial subset of properties of natural rat hearts. The current study provided a method for fabrication of a BAH and revealed challenges toward BAH fabrication with functional performance metrics of natural mammalian hearts.

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“…These applications are the key for the future treatment of many diseases [10]. Currently, in tissue engineering are several scaffolds materials under investigation.…”

Section: Discussionmentioning

confidence: 99%

Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering

Aleksieva¹,

Hollweck²,

Thierfelder³

et al. 2012

BioMed Eng OnLine

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BackgroundTissue engineering represents a promising new method for treating heart valve diseases. The aim of this study was evaluate the importance of conditioning procedures of tissue engineered polyurethane heart valve prostheses by the comparison of static and dynamic cultivation methods.MethodsHuman vascular endothelial cells (ECs) and fibroblasts (FBs) were obtained from saphenous vein segments. Polyurethane scaffolds (n = 10) were primarily seeded with FBs and subsequently with ECs, followed by different cultivation methods of cell layers (A: static, B: dynamic). Group A was statically cultivated for 6 days. Group B was exposed to low flow conditions (t1= 3 days at 750 ml/min, t2= 2 days at 1100 ml/min) in a newly developed conditioning bioreactor. Samples were taken after static and dynamic cultivation and were analyzed by scanning electron microscopy (SEM), immunohistochemistry (IHC), and real time polymerase chain reaction (RT-PCR).ResultsSEM results showed a high density of adherent cells on the surface valves from both groups. However, better cell distribution and cell behavior was detected in Group B. IHC staining against CD31 and TE-7 revealed a positive reaction in both groups. Higher expression of extracellular matrix (ICAM, Collagen IV) was observed in Group B. RT- PCR demonstrated a higher expression of inflammatory Cytokines in Group B.ConclusionWhile conventional cultivation method can be used for the development of tissue engineered heart valves. Better results can be obtained by performing a conditioning step that may improve the tolerance of cells to shear stress. The novel pulsatile bioreactor offers an adequate tool for in vitro improvement of mechanical properties of tissue engineered cardiovascular prostheses.

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Getting to the Heart of Tissue Engineering

Cited by 13 publications

References 162 publications

Trophic Actions of Bone Marrow-Derived Mesenchymal Stromal Cells for Muscle Repair/Regeneration

Trophic Actions of Bone Marrow-Derived Mesenchymal Stromal Cells for Muscle Repair/Regeneration

Establishing the Framework for Fabrication of a Bioartificial Heart

Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering

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