Bioreduction of Chromate by an Isolated Bacillus anthracis Cr-4 with Soluble Cr(III) Product

Xu, Wenji; Jian, Hao; Liu, Yunguo; Zeng, Guangming; Li, Xin; Zhang, Wei

doi:10.1007/s11270-015-2356-z

Cited by 32 publications

(8 citation statements)

References 31 publications

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“…Similar to our ndings, the cells were inhibited due to the increase in Cr(VI) toxicity on Ochrobactrum sp. (He et al 2009), B. anthracis (Xu et al 2015), and Microbacterium paraoxydans (Mishra et al 2021) strains due to the increase in Cr(VI) concentration.…”

Section: Discussionmentioning

confidence: 99%

Bioreduction and Bioremoval of Hexavalent Chromium by Genetically Engineered Strains (Escherichia Coli MT2A and Escherichia Coli MT3)

Akkurt¹,

Oğuz²,

Uçkun³

2021

Preprint

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Studies on the removal of toxic metals from water by genetic engineering applications are becoming more and more diverse. Especially by the expression of cysteine and thiol rich proteins such as metallothioneins in bacterial cells, a high rate of removal of metal ions from the environment is ensured. In this study, we evaluated the removal and reduction of hexavalent chromium Cr(VI)from aqueous solutions with the recombinant strains obtained by cloning the human metallothioneinsMT2A and MT3 into Escherichia coli Jm109.E. coli MT2A was the most effective strain in both Cr(VI) removal (89%in 25 mg/L Cr(VI) application) and Cr(VI) reduction (76%in 25 mg/L Cr(VI) application).Cr(VI) adsorbed per dry cell (at 25 mg/L) were 17 mg/g, 22 mg/g and 19 mg/g for Jm109, MT2A and MT3, respectively. Scanning electron microscope (SEM) images showed that the morphological structures of Cr(VI)treated cells were significantly damaged compared to control cells. Scanning transmission electron microscope (STEM) images showed black spots in the cytoplasm of cells treated with Cr(VI).From the shifts in the Fourier transform infrared spectroscopy analysis (FTIR) spectra of the cells treated with Cr(VI) compared to the control cells, it was determined that the groups interacting with Cr were amino, hydroxyl, methyl and sulfhydryl. When all these experimental data were evaluated together, it was concluded that all three species were effective in removing Cr(VI) from aqueous solutions and MT2A strain could be used as the most effective biotechnological tool.

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Section: Discussionmentioning

confidence: 99%

Bioreduction and Bioremoval of Hexavalent Chromium by Genetically Engineered Strains (Escherichia Coli MT2A and Escherichia Coli MT3)

Akkurt¹,

Oğuz²,

Uçkun³

2021

Preprint

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“…According Tan et al [18], and Huang et al [40], the increase in Cr(VI) reduction caused by Cu 2+ is due to the fact that it is one of the essential components of some antioxidiants, such as superoxide dismutase and catalase. Moreover, it acts as an electron transporter for oxidative respiratory system [41]. At a higher heavy metal concentration of 50 mg/L, a significant inhibition of Cr(VI) reduction was observed in the presences of Ni 2+ and Pb 2+ , with 81 and 43% Cr(VI) reduction being achieved, respectively, whereas the presence of Mn 2+ had no effect.…”

Section: Effects Of Coexisting Heavy Metals On Cr(vi) Reductionmentioning

confidence: 99%

“…To quantitatively determine the interaction between the reduction rate and time for Cr(VI) removal by the bacterium consortia coming from a wastewater treatment plant, the kinetics for Cr(VI) reduction were conducted. The kinetics of the Cr(VI) bioreduction at varying initial Cr(VI) concentrations was studied by first-and second-order exponential decay [11,17,[39][40][41][42]. k 1 was determined as the slope from plotting ln([Cr(VI)]/[Cr(VI) 0 ]) versus time.…”

Section: Kinetics Of Cr(vi) Reduction By Bacteria Consortiamentioning

confidence: 99%

Evaluation of Cr(VI) Reduction Using Indigenous Bacterial Consortium Isolated from a Municipal Wastewater Sludge: Batch and Kinetic Studies

2021

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Hexavalent Chromium (Cr(VI)) has long been known to be highly mobile and toxic when compared with the other stable oxidation state, Cr(III). Cr(VI)-soluble environmental pollutants have been detected in soils and water bodies receiving industrial and agricultural waste. The reduction of Cr(VI) by microbial organisms is considered to be an environmentally compatible, less expensive and sustainable remediation alternative when compared to conventional treatment methods, such as chemical neutralization and chemical precipitation of Cr. This study aims to isolate and identify the composition of the microbial consortium culture isolated from waste activated sludge and digested sludge from a local wastewater treatment plant receiving high loads of Cr(VI) from an abandoned chrome foundry in Brits (North Waste Province, South Africa). Furthermore, the Cr(VI) reduction capability and efficiency by the isolated bacteria were investigated under a range of operational conditions, i.e., pH, temperature and Cr(VI) loading. The culture showed great efficiency in reduction capability, with 100% removal in less than 4 h at a nominal loading concentration of 50 mg Cr(VI)/L. The culture showed resilience by achieving total removal at concentrations as high as 400 mg Cr(VI)/L. The consortia exhibited considerable Cr(VI) removal efficiency in the pH range from 2 to 11, with 100% removal being achieved at a pH value of 7 at a 37 ± 1 °C incubation temperature. The time course reduction data fitted well on both first and second-order exponential rate equation yielding first-order rate constants in the range 0.615 to 0.011 h−1 and second order rate constants 0.0532 to 5 × 10−5 L·mg−1·h−1 for Cr(VI) concentration of 50–400 mg/L. This study demonstrated the bacterial consortium from municipal wastewater sludge has a high tolerance and reduction ability over a wide range of experimental conditions. Thus, show promise that bacteria could be used for hexavalent chromium remediate in contaminated sites.

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“…The maximum growth and Cr(VI bioreduction were detected at pH 9.0 and pH 10, respectively, showing Cr(VI) removal of 58.1% and 78.9% of the initial Cr(VI), respectively. However, at pH 5 and 12, the Cr(VI) bioremoval was severely decreased/inhibited, showing Cr(VI) removal of 15.6% and 8.5%, respectively, which is mostly due to the adverse effect of very low/high pH on bacterial growth and metabolic activities (Lin et al 2012;Xu et al 2015). In addition, the structure of microbial sulfate transporter is highly affected in extreme acidic medium and hence inhibit the intracellular Cr(VI) reductase activity (Khattar et al 2014).…”

Section: Effect Of Phmentioning

confidence: 99%

“…In addition, Cu 2+ plays an essential role in the electron transport chain system in the oxidative respiratory system in bacteria, furthermore, it is a component of several antioxidant enzymes such as catalase and superoxide dismutase. Therefore, Cu 2+ could improve the bacterial tolerance to Cr(VI) and the electron transfer efficiency, thus enhancing the Cr(VI) detoxification (Xu et al 2015).…”

Section: Effect Of Co-existing Ionsmentioning

confidence: 99%

Bioreduction of hexavalent chromium by a novel haloalkaliphilic Salipaludibacillus agaradhaerens strain NRC-R isolated from hypersaline soda lakes

et al. 2021

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A novel Cr(VI)-resistant haloalkaliphilic bacterial strain NRC-R, identified as Salipaludibacillus agaradhaerens, was isolated from hypersaline soda lakes and characterized for its Cr(VI) bioreduction efficiency. Strain NRC-R grew well and effectively reduced Cr(VI) under a wide range of sodium chloride, pH, shaking velocity and temperature, showing maximum Cr(VI) reduction at 8% NaCl, pH 10, 150 rpm and 35 °C, respectively. Strain NRC-R was able to grow and reduce Cr(VI) effectively in the presence of different heavy metals and oxyanions (Pb 2+ , Zn 2+ , Co 2+ , Mn 2+ , Ni 2+ , Mo 2+ , HPO 4 − , NO 3 − , SO 4 2− and HCO 3 − ). Furthermore, Fe 3+ and Cu 2+ significantly enhanced the Cr(VI) removal by about 1.5 fold. Strain NRC-R could reduce Cr(VI) using a variety of electron donors, exhibiting a maximum reduction in the presence of NADH and fructose. The bioremoval of Cr(VI) using strain NRC-R was due to direct enzymatic reduction and the chromate reductase activity was mainly detected in the bacterial cell membrane. Under the optimized conditions, strain NRC-R showed a remarkable Cr(VI) bioreduction with highest reduction rate of 240 uM/h. Cr(VI) concentrations of up to 3 mM (888.5 mg/L) and 4 mM (1177 mg/L) were completely reduced within 16 h and 32 h, respectively. TEM and SEM-EDX analyses confirmed the biosorption of chromium species into the cells. To the best of our knowledge, this is the first report about Cr(VI) reduction by S. agaradhaerens. In conclusion, S. agaradhaerens NRC-R was a highly efficient Cr(VI) reducing haloalkaliphilic bacterium that has a significant potential in the bioremediation of Cr(VI)-contaminated environments.

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Bioreduction of Chromate by an Isolated Bacillus anthracis Cr-4 with Soluble Cr(III) Product

Cited by 32 publications

References 31 publications

Bioreduction and Bioremoval of Hexavalent Chromium by Genetically Engineered Strains (Escherichia Coli MT2A and Escherichia Coli MT3)

Bioreduction and Bioremoval of Hexavalent Chromium by Genetically Engineered Strains (Escherichia Coli MT2A and Escherichia Coli MT3)

Evaluation of Cr(VI) Reduction Using Indigenous Bacterial Consortium Isolated from a Municipal Wastewater Sludge: Batch and Kinetic Studies

Bioreduction of hexavalent chromium by a novel haloalkaliphilic Salipaludibacillus agaradhaerens strain NRC-R isolated from hypersaline soda lakes

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