Bioremediation of a weathered and a recently oil-contaminated soils from Brazil: a comparison study

Trindade, P.V.O.; Sobral, Luis Gonzaga Santos; Rizzo, Andrea C.L.; Leite, Selma Gomes Ferreira; Soriano, Adriana U.

doi:10.1016/j.chemosphere.2004.09.021

Cited by 171 publications

(94 citation statements)

References 13 publications

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“…This ability of WatG to degrade n-alkanes in diesel oil in soil microcosms is comparable to that of a bacterial consortium consisting of Nocardia nova and Rhodotorula glutinis var. dairensis [11] and to that of active sewage sludge [5]. Thus it is considered that WatG can be adopted to a practical single degrader of petroleum products under aerobic conditions in the soil environment.…”

Section: Resultsmentioning

confidence: 99%

Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms

Ueno

Hasanuzzaman²,

Yumoto

et al. 2006

Curr Microbiol

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Degradation of n-alkanes in diesel oil by Pseudomonas aeruginosa strain WatG (WatG) was verified in soil microcosms. The total petroleum hydrocarbon (TPH) degradation level in two bioaugmentation samples was 51% and 46% for 1 week in unsterilized and sterilized soil microcosms, respectively. The TPH degradation in the biostimulation was of control level (15%). The TPH degradation in aeration-limited samples was clearly reduced when compared with that in aeration-unlimited ones under both sterilized and unsterilized conditions. Addition of WatG into soil microcosms was accompanied by dirhamnolipid production only in the presence of diesel oil. These findings suggest that degradation of n-alkanes in diesel oil in soil microcosms would be facilitated by bioaugmentation of WatG, with production of dirhamnolipid, and also by participation of biostimulated indigenous soil bacteria

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Section: Resultsmentioning

confidence: 99%

Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms

Ueno

Hasanuzzaman²,

Yumoto

et al. 2006

Curr Microbiol

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“…Based on this concept and using the accumulated value of CO 2 generated during 30 days of the experiments, the biodegradation efficiency (BE) was calculated as: BE% = (Totally biodegraded carbon) 100 / Total organic carbon in the soil; Totally biodegraded carbon = 2 Carbon from CO 2 generated during 30 days. 7 It is also frequently assumed that the total organic carbon corresponds to 80 to 90% of total petroleum hydrocarbon content in the contaminated soil with crude oil. Taking this into account it is possible to confirm in Table 1 that the value obtained for organic carbon (C organic ) corresponds to 89% of the Total Petroleum Hydrocarbon (TPH) present in the weathered contaminated soil.…”

Section: Determinationmentioning

confidence: 99%

“…dairenesis), previously isolated from the crude oil contaminated soil. 6 Cells were grown in mineral medium containing crude oil (4% v/v) as the sole carbon source, centrifuged and washed twice with sterile saline solution (0.7%), as described by Trindade et al 7 Inoculum was added to the soil samples in order to get the concentration of 10 8 CFU (Colony Forming Unit) g of soil -1 .…”

Section: Inoculum Productionmentioning

confidence: 99%

“…6,7 A second inoculum type, called Hydrocarbon Degrader Consortium (HDC), was used in both testes such as soil microcosms and liquid medium. These inoculum was constituted by two crude oil-degrading microorganisms (Nocardia nova and Rhodotorula glutinis var.…”

Section: Inoculum Productionmentioning

confidence: 99%

“…This included, for example, using of bioaumentantion and/ or biostimulation techniques, evaluation of the optimal moisture content and pH that stimulates de microbial activity, and so on. [5][6][7] Nevertheless, the results obtained in terms of organic pollutants removal (expressed as total petroleum hydrocarbon -TPH-removal) and biodegradation efficiency (based on the carbon dioxide generation as a result of aerobic metabolism) were considered low, ranging from 5 to 50 % and 5 to 14%, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Preliminary identification of the bioremediation limiting factors of a clay bearing soil contaminated with crude oil

Rizzo¹,

Cunha²,

Santos³

et al. 2008

J. Braz. Chem. Soc.

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A biorremediação é uma alternativa atraente para o tratamento de solos contaminados com hidrocarbonetos de petróleo. No entanto, a efetividade do processo pode ser limitada não só pelas características do contaminante, mas também pela sua biodisponibilidade. Este trabalho tem como meta o estabelecimento de procedimento preliminar para identificar o fator principal (recalcitrância ou biodisponibilidade) que interfere negativamente na biodegradação, resultando em baixas eficiên-cias. Foram realizados testes em microcosmos contendo solo aplicando as técnicas de bioestímulo e bioaumento. Experimentos semelhantes foram conduzidos em meio líquido contendo óleo cru e microorganismos extraídos do solo contaminado, nutrientes e inóculo microbiano (Rhodoturula glutinis e Nocardia nova). Após 30 dias, o maior nível de biodegradação (98.8%) foi obtido na condição com bioaumento em meio líquido, indicando que o problema principal de biodegradação de hidrocarbonetos de petróleo pode estar associado à baixa biodisponibilidade destes no solo e não apenas a recalcitrância intrínseca do poluente residual.Bioremediation is an attractive alternative to treat soils contaminated with petroleum hydrocarbons. However, the effectiveness of biodegradation process can be limited by both contaminant characteristics and its bioavailability in soil. This work aims at establishing a preliminary procedure to identify the main factor (hydrocarbon recalcitrance or its bioavailability) that impairs the biodegradation, possibly resulting in low remediation efficiencies. Tests in soil microcosms were carried out applying biostimulation and bioaugmentation techniques. Similar experiments were conducted using liquid medium containing crude oil and microorganisms, both of them extracted from a weathered contaminated soil sample, nutrients and microbial inoculum constituted of Rhodoturula glutinis and Nocardia nova. After 30 days, highest microcosms biodegradation levels (98.8%) were obtained with bioaugmentation in liquid medium, pointing out that the main problem of petroleum hydrocarbon utilization by microorganisms was associated to its availability and not only to the intrinsic recalcitrancy of remaining pollutants.

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Biodegradation of Kerosene and Diesel

Speight¹,

Arjoon²

2012

Bioremediation of Petroleum and Petroleum Products

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Bioremediation of a weathered and a recently oil-contaminated soils from Brazil: a comparison study

Cited by 171 publications

References 13 publications

Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms

Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms

Preliminary identification of the bioremediation limiting factors of a clay bearing soil contaminated with crude oil

Biodegradation of Kerosene and Diesel

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