This study investigated the leaching potential of diuron and linuron in different soil types in Gaza Strip, Palestine under laboratory and field conditions. Leaching potential was evaluated by tin columns and bioassay technique using Molokhia as test plant. The responses of the test plant were regressed versus concentrations of the herbicide to estimate linear regression equation and the regression coefficient. The obtained results indicate strong positive association between Molokhia and concentrations of diuron or linuron; accordingly it was used in the bioassay and estimation of the concentrations of the tested herbicides in the leaching depth. Leaching potential was very low in North Gaza and Kkan Younis soil, whereas in the Meddle zone soil was very large. Leaching potential under laboratory conditions was larger that under field conditions. The bioestimated concentrations in soil layer under field conditions were sever folds of magnitude lower than those under laboratory conditions. The interesting outcome of this study is that leaching potential is dependent on soil clay fraction, soil organic matter, and soil pH. These results provided answers to the questions raised by farmers in Gaza Strip.