Bioremediation of Soil Artificially Contaminated with Petroleum Hydrocarbon Oil Mixtures: Evaluation of the Use of Animal Manure and Chemical Fertilizer

Abstract

The search for cheaper and environmentally friendly options of enhancing petroleum hydrocarbon degradation has continued to elicit research interest. One of such options is the use of animal manure as biostimulating agents. A combination of treatments consisting of the application of poultry manure, piggery manure, goat manure, and chemical fertilizer was evaluated in situ during a period of 4 weeks of remediation. Each treatment contained petroleum hydrocarbon mixture (kerosene, diesel oil, and gasoline mixtures) (10% w/w) in soil as a sole source of carbon and energy. After 4 weeks of remediation, the results showed that poultry manure, piggery manure, goat manure, and NPK (nitrogen, phosphorous, and potash [potassium]) fertilizer exhibited 73%, 63%, 50%, and 39% total petroleum hydrocarbon degradation, respectively. Thus, all the biostimulating treatment strategies showed the ability to enhance petroleum hydrocarbon microbial degradation. However, poultry manure, piggery manure, and goat manure treatments showed greater petroleum hydrocarbon reductions than NPK fertilizer treatment. A first-order kinetic equation was fitted to the biodegradation data and the specific degradation rate constant (k) values obtained showed that the order of effectiveness of these biostimulating strategies in the cleanup of soil contaminated with petroleum hydrocarbon mixtures (mixture of kerosene, diesel oil, and gasoline) is NPK fertilizer < goat manure < piggery manure < poultry manure. Therefore, this present work has indicated that the application of poultry manure, piggery manure, goat manure, and chemical fertilizer could enhance petroleum hydrocarbon degradation with poultry manure, showing a greater effectiveness and thus could be one of the severally sought environmentally friendly ways of remediating natural ecosystem contaminated with crude oil.

Statistical optimization and kinetic studies of enhanced bioremediation of crude oil - contaminated marine water using combined adsorption-biostimulation strategy

“…This observation indicates that the use of organic fertilizer enhanced the TPH reduction in crude oil-contaminated marine water. Similar observations have been reported for the use of organic fertilizer (of different forms) which include animal waste such as cow dung (Umanu et al, 2014;Obiakalaije et al, 2015;Chikere et al, 2016), poultry droppings/dung (Obiakalaije et al, 2015;Chikere et al, 2016), goat dung (Agarry et al, 2010;Obiakalaije et al, 2015), pig dung (Agarry et al, 2013), reptile waste such as uric acid based waste or guano fertilizer (Knezevich et al, 2007) and agricultural crop/plant residue like peanut hull powder (Xu and Lu, 2010), spent mushroom (Garcia-Delgado et al, 2015) in the bioremediation of soil and sediment contaminated with petroleum hydrocarbon/crude oil. The effects of slow release inorganic NPK fertilizer (factor B) on percent TPH reduction is shown in Figure 1 (b).…”

Biodegradation of Bitumen in Soil and Its Enhancement by Inorganic Fertilizer and Oxygen Release Compound: Experimental Analysis and Kinetic Modelling

“…These observations indicates that degradation of petroleum hydrocarbons in bitumen was relatively higher at a greater amount of inorganic NPK fertilizer and that amendment with inorganic fertilizer enhanced the biodegradation (biostimulation) in comparison with the unamended contaminated soil (natural attenuation). Similar observations have been reported for the use of NPK fertilizer in the biodegradation of crude oil [16], petroleum hydrocarbon mixtures [17] and fuel oil, diesel and kerosene [12]. It has been reported that the addition of nitrogenous nutrient enhances biodegradation of hydrocarbon-polluted soil, presumably by removing the nitrogen limitation resulting from low natural level [40,42].…”

“…The effects of nutrients (i.e. NPK), aeration and biostimulation of indigenous soil microorganisms and inoculation of extraneous microbial consortia on the bioremediation of oil contaminated soil have been investigated [16,17].…”

Evaluation of the effects of inorganic and organic fertilizers and activated carbon on bioremediation of soil contaminated with weathered crude oil

“…This is in agreement with the observations of Ibiene et al (2011), Nduka et al (2012, Chikere et al (2012) and Obiakalaije et al (2015) who respectively used poultry droppings and/or cow dung as organic fertilizer (biostimulation agents) in the remediation of petroleum hydrocarbon contaminated environment. Agarry et al (2010) and Agarry et al (2013b) had in their previous studies also reported the enhancement of bioremediation in soil contaminated with petroleum hydrocarbons using cow dung, pig dung, poultry dung as well as the mixture of cow dung and pig dung, respectively.…”