Bioresorbable glass effect on the physico-chemical properties of bilayered scaffolds for osteochondral regeneration

Gentile, Piergiorgio; Chiono, Valeria; Tonda‐Turo, Chiara; Mattu, Clara; Baino, Francesco; Brovarone, Chiara Vitale; Ciardelli, Gianluca

doi:10.1016/j.matlet.2012.08.023

Cited by 19 publications

(11 citation statements)

References 22 publications

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“…Low mechanical strength of the scaffolds may be a concern in the field of tissue engineering. To address this weakness the following improvements may be recommended: (1) modification of the gas foaming method to reduce the pore size so that more desirable tensile strength can be achieved, and (2) addition of components with higher tensile strength such as bioceramics and bioglasses which are reported to be effective in improving the tensile strength of the gelatin structure [20,64].…”

Section: Discussionmentioning

confidence: 99%

Gelatin porous scaffolds fabricated using a modified gas foaming technique: Characterisation and cytotoxicity assessment

Poursamar

Hatami

Lehner

et al. 2015

Materials Science and Engineering: C

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The current study presents an effective and simple strategy to obtain stable porous scaffolds from gelatin via gas foaming method. The technique exploits the intrinsic foaming ability of gelatin in the presence of CO2 to obtain a porous structure stabilised with glutaraldehyde. The produced scaffolds were characterised using physical and mechanical characterisation methods. The results showed that gas foaming may allow the tailoring of the 3-dimensional structure of the scaffolds with an interconnected porous structure. To assess the effectiveness of preparation method in mitigating the potential cytotoxicity risk of using glutaraldehyde as a crosslinker, direct and indirect cytotoxicity assays were performed at different concentrations of glutaraldehyde. The results indicate the potential of the gas foaming method, in the preparation of viable tissue engineering scaffolds.

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Section: Discussionmentioning

confidence: 99%

Gelatin porous scaffolds fabricated using a modified gas foaming technique: Characterisation and cytotoxicity assessment

Poursamar

Hatami

Lehner

et al. 2015

Materials Science and Engineering: C

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“…Nanosized inorganic particles provide a higher specific surface area and thus a higher interface area that might enhance the interfacial bonding strength between the inorganic filler particles and the polymer matrix. Generally, BGs in the system of CaO–P 2 O 5 –SiO 2 are attractive as inorganic materials for bone tissue engineering as comprehensively discussed in the literature . Addition of BGs to biodegradable polymeric materials for forming composite scaffolds generally enhances bioactivity, controls degradation rate, and provides stiffness increasing also the structural integrity of the resulting scaffold .…”

Section: Introductionmentioning

confidence: 99%

Combining Collagen and Bioactive Glasses for Bone Tissue Engineering: A Review

Sarker

Hum

Nazhat

et al. 2014

Adv Healthcare Materials

121

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Collagen (COL), the most abundant protein in mammals, offers a wide range of attractive properties for biomedical applications which are the result of its biocompatibility and high affinity to water. However, due to the relative low mechanical properties of COL its applications are still limited. To tackle this disadvantage of COL, especially in the field of bone tissue engineering, COL can be combined with bioactive inorganic materials in a variety of composite systems. One of such systems is the collagen-bioactive glass (COL-BG) composite family, which is the theme of this Review. BG fillers can increase compressive strength and stiffness of COL-based structures. This article reviews the relevant literature published in the last 15 years discussing the fabrication of a variety of COL-BG composites. In vitro cell studies have demonstrated the osteogenic, odontogenic, and angiogenic potential of these composite systems, which has been confirmed by stimulating specific biochemical indicators of relevant cells. Bony integration and connective tissue vessel formation have also been studied by implantation of the composites in vivo. Areas of future research in the field of COL-BG systems, based on current challenges, and gaps in knowledge are highlighted.

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“…Phosphate-based resorbable glass materials are proposed to have hard tissue engineering applications. [9][10][11] Recently, gallium-containing phospho-silicate glasses based on Bioglass 45S5 were found to retain bioactivity during in vitro analyses. 12,13 But the degradation rates of these glasses are reported to be lower compared with gallium-incorporated phosphate-based glasses (Ga-PBG) in the CaO-Na 2 O-P 2 O 5 system.…”

Section: Introductionmentioning

confidence: 99%

Potential use of gallium-doped phosphate-based glass material for periodontitis treatment

et al. 2015

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This study aimed at evaluating the potential effect of gallium-incorporated phosphate-based glasses towards periodontitis-associated bacteria, Porphyromonas gingivalis, and matrix metalloproteinase-13. Periodontitis describes a group of inflammatory diseases of the gingiva and supporting structures of the periodontium. They are initiated by the accumulation of plaque bacteria, such as the putative periodontal pathogen Porphyromonas gingivalis, but the host immune response such as elevated matrix metalloproteinases are the major contributing factor for destruction of periodontal tissues. Antibacterial assays of gallium-incorporated phosphate-based glasses were conducted on Porphyromonas gingivalis ATCC 33277 using disc diffusion assay on fastidious anaerobe agar and liquid broth assay in a modified tryptic soy broth. In vitro study investigated the effect of gallium on purified recombinant human matrix metalloproteinase-13 activity using matrix metalloproteinase assay kit. In vivo biocompatibility of gallium-incorporated phosphate-based glass was evaluated in rats as subcutaneous implants. Antibacterial assay of gallium displayed activity against Porphyromonas gingivalis (inhibition zone of 22 ± 0.5 mm compared with 0 mm for control glass, c-PBG). Gallium in the glass contributed to growth inhibitory effect on Porphyromonas gingivalis (up to 1.30 reductions in log 10 values of the viable counts compared with control) in a modified tryptic soy broth. In vitro study showed gallium-incorporated phosphate-based glasses inhibited matrix metalloproteinase activity significantly (p ≤ 0.01) compared with c-PBG. Evaluation of in vivo biocompatibility of gallium-incorporated phosphate-based glasses in rats showed a non-toxic and foreign body response after 2 weeks of implantation. The results indicate that gallium ions might act on multiple targets of biological mechanisms underlying periodontal disease. Moreover, gallium-incorporated phosphate-based glasses are biocompatible in a rat model. The findings warrant further investigation and will have important clinical implications in the future treatment and management of periodontitis.

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Bioresorbable glass effect on the physico-chemical properties of bilayered scaffolds for osteochondral regeneration

Cited by 19 publications

References 22 publications

Gelatin porous scaffolds fabricated using a modified gas foaming technique: Characterisation and cytotoxicity assessment

Gelatin porous scaffolds fabricated using a modified gas foaming technique: Characterisation and cytotoxicity assessment

Combining Collagen and Bioactive Glasses for Bone Tissue Engineering: A Review

Potential use of gallium-doped phosphate-based glass material for periodontitis treatment

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