Biosafety

Chisti, Yusuf

doi:10.1002/9783527619641.ch30

Cited by 4 publications

(2 citation statements)

References 37 publications

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“…For this dinoflagellate, any further scale up of production must rely on multiplying the number of photobioreactors. The downstream extraction-purification steps of the KTX process can be easily scaled-up to a multi-kilogram level as commercial chromatographic separations technology applicable to this scale is already in use in other biotechnology processes (Chisti, 2007). The bioprocess developed in the present work, used only readily accessible and inexpensive materials and solvents to minimize the cost of production.…”

Section: Resultsmentioning

confidence: 99%

Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract

López-Rosales

Sánchez-Mirón

Camacho

et al. 2018

Bioresource Technology

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A pilot-scale bioprocess was developed for the production of karlotoxin-enriched extracts of the marine algal dinoflagellate Karlodinium veneficum. A bubble column and a flat-panel photobioreactors (80-281 L) were used for comparative assessment of growth. Flow hydrodynamics and energy dissipation rates (EDR) in the bioreactors were characterized through robust computational fluid dynamic simulations. All cultures were conducted monoseptically outdoors. Bubble column (maximum cell productivity in semicontinuous operation of 58 × 10 cell mL day) proved to be a better culture system for this alga. In both reactors, the local EDR near the headspace, and in the sparger zone, were more than one order of magnitude higher than the average value in the whole reactor (=4 × 10 W kg). Extraction of the culture and further purification resulted in the desired KTXs extracts. Apparently, the alga produced three congeners KTXs: KmTx-10 and its sulfated derivative (sulfo-KmTx-10) and KmTx-12. All congeners possessed hemolytic activity.

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Section: Resultsmentioning

confidence: 99%

Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract

López-Rosales

Sánchez-Mirón

Camacho

et al. 2018

Bioresource Technology

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“…A similar yield of about 45% and purity [97% were attained by Zhang et al (1999) in recovering recombinant streptokinase from inclusion bodies produced in E. coli. Generalized schemes for recovery and renaturation of inclusion body recombinant proteins have been published (Chisti 1998a).…”

Section: Recovery and Purificationmentioning

confidence: 99%