Biosensing Based on Light Absorption of Nanoscaled Gold and Silver Particles

Frederix, Filip; Friedt, Jean‐Michel; Choi, Kang‐Hoon; Laureyn, Wim; Campitelli, T. Andrew; Mondelaers, D.; Maes, Guido; Borghs, Gustaaf

doi:10.1021/ac0346609

Cited by 357 publications

(233 citation statements)

References 18 publications

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“…The shifting of wavelength could be due to the change of index of refraction around the Ag NPs induced by both JEV antibodies and BSA (Sharma et al 2012), which also indicated successful immobilization of JEV antibody onto the Ag NPs-based sensing probe (El-Sayed et al 2005). The binding of JEV antigen onto antibody immobilized Ag NPs-based sensing probes had further enhanced the intensity of surface plasmon resonance absorption at the wavelength of 427 nm (Figure 6d) (Frederix et al 2003).…”

Section: Characterization Of Ag Nps-based Sensing Probesmentioning

confidence: 86%

A Novel Silver Nanoparticles-based Sensing Probe for the Detection of Japanese Encephalitis Virus Antigen

Lim¹,

Chin²,

Pang³

et al. 2017

JSM

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Section: Characterization Of Ag Nps-based Sensing Probesmentioning

confidence: 86%

A Novel Silver Nanoparticles-based Sensing Probe for the Detection of Japanese Encephalitis Virus Antigen

Lim¹,

Chin²,

Pang³

et al. 2017

JSM

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“…The formation of the complex is evidenced from the color change (detectable by human eye) and the appearance of a peak at around 770 nm (Barman et al 2014a, b;Li et al 2013;Frederix et al 2003 , though no precipitation occurred, but they could not produce any significant color change and thus no change in the UV-Visible absorption spectrum (Fig. 2C).…”

Section: Optimization Of Synthesized Agnpmentioning

confidence: 97%

Detection of heavy metals (Cu+2, Hg+2) by biosynthesized silver nanoparticles

2015

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Here, we are reporting two methods for detection of Cu ?2 ion and Hg ?2 ions using biosynthesized silver nanoparticles. The detection of Cu ?2 ion was based on changes in absorbance resulting from complex formation of the metal ion. Various concentrations of Cu ?2 ion were used to test the linearity and sensitivity of the method. A new peak at around 770 nm, in addition to the peak of the AgNP at 406 nm, was observed in each case (above 20 lM). With the increase of concentration of Cu ?2 ion solution, the absorbance at 406 nm peak decreased and that of 770 nm increased gradually. The calibration curve obtained from the ratio of the absorption coefficients of these two peaks (Ex 770/406 ) versus concentration of Cu ?2 ions enables one to estimate quantitatively the amount of Cu ?2 ions present in water in lM levels. This AgNP was further functionalized with 3-mercapto-1, 2-propanediol (MPD) for detection of Hg ?2 present in water by colorimetric method. As soon as Hg ?2 solution was added in MPDfunctionalized AgNP (MPD-AgNP), a new peak at around 606 nm appeared along with the peak at 404 nm. The new peak might be due to the aggregations occurred by the recognition of heavy metal ion Hg ?2 by MPD-AgNP through dipropionate ion. A calibration curve between the ratios of the absorption coefficients of these two peaks (Ex 404/606 ) and concentration of Hg ?2 was drawn for quantitative estimation of Hg ?2 present in water at lM level.

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“…Our results showed that the intensity of SP band of gold was increased after the exposure of biotin-functionalized gold nanoisland films to streptavidin solution (100 µg/mL) in PBS for 2 min (Figure 6). 47 No further changes were observed from the longer exposure of island films to streptavidin solution. In comparison, non-specific binding studies using MUA-functionalized gold nanoisland films showed no such change on the intensity of SP band of gold.…”

Section: Formation and Characterization Of Gold Nanoisland Filmsmentioning

confidence: 98%

Preparation of Gold Nanoisland Arrays from Layer-by-Layer Assembled Nanoparticle Multilayer Films

Choi¹,

Guerrero²,

Aquino³

et al. 2010

Bulletin of the Korean Chemical Society

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This article introduces a facile nanoparticle self-assembly/annealing method for the preparation of nanoisland films. First, nanoparticle-polymer multilayer films are prepared with layer-by-layer assembly. Nanoparticle multilayer films are then annealed at ~ 500 o C in air to evaporate organic matters from the films. During the annealing process, the nanoparticles on the solid surface undergo nucleation and coalescence, resulting in the formation of nanostructured gold island arrays. By controlling the overall thickness (number of layers) of nanoparticle multilayer films, nanoisland films with various island density and different average sizes are obtained. The surface property of gold nanoisland films is further controlled by the self-assembly of alkanethiols, which results in an increased surface hydrophobicity of the films. The structure and characteristics of these nanoisland film arrays are found to be quite comparable to those of nanoisland films prepared by vacuum evaporation method. However, this self-assembly/annealing protocol is simple and requires only common laboratory supplies and equipment for the entire preparation process.

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Biosensing Based on Light Absorption of Nanoscaled Gold and Silver Particles

Cited by 357 publications

References 18 publications

A Novel Silver Nanoparticles-based Sensing Probe for the Detection of Japanese Encephalitis Virus Antigen

A Novel Silver Nanoparticles-based Sensing Probe for the Detection of Japanese Encephalitis Virus Antigen

Detection of heavy metals (Cu+2, Hg+2) by biosynthesized silver nanoparticles

Preparation of Gold Nanoisland Arrays from Layer-by-Layer Assembled Nanoparticle Multilayer Films

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