Biosensing Membrane Base on Ferulic Acid and Glucose Oxidase for an Amperometric Glucose Biosensor

Abstract

A biosensing membrane base on ferulic acid and glucose oxidase is synthesized onto a carbon paste electrode by electropolymerization via cyclic voltammetry in aqueous media at neutral pH at a single step. The developed biosensors exhibit a linear response from 0.082 to 34 mM glucose concentration, with a coefficient of determination R2 equal to 0.997. The biosensors display a sensitivity of 1.1 μAmM−1 cm−2, a detection limit of 0.025 mM, and 0.082 mM as glucose quantification limit. The studies reveal stable, repeatable, and reproducible biosensors response. The results indicate that the novel poly-ferulic acid membrane synthesized by electropolymerization is a promising method for glucose oxidase immobilization towards the development of glucose biosensors. The developed glucose biosensors exhibit a broader linear glucose response than other polymer-based glucose biosensors.

Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring

“…Considering Clark's study, many glucose-sensing electrodes were initially developed under the consideration of being based on enzyme membranes, which exhibit advantages such as high selectivity and high linearity [44]. For example, Valdés-Ramírez and Galicia synthesized biosensing membranes of ferulic acid (FA) and glucose oxidase on carbon paste electrodes via an electropolymerization reaction in aqueous media at neutral pH [45]. The results show that the novel poly ferulic acid membrane synthesized by electropolymerization is a promising method for immobilization of glucose oxidase and the developed glucose biosensor exhibits a wider linear glucose response compared with other polymer-based glucose biosensors (Figure 2A).…”

Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring

“…Considering Clark’s study, many glucose-sensing electrodes were initially developed under the consideration of being based on enzyme membranes, which exhibit advantages such as high selectivity and high linearity [ 44 ]. For example, Valdés-Ramírez and Galicia synthesized biosensing membranes of ferulic acid (FA) and glucose oxidase on carbon paste electrodes via an electropolymerization reaction in aqueous media at neutral pH [ 45 ]. The results show that the novel poly ferulic acid membrane synthesized by electropolymerization is a promising method for immobilization of glucose oxidase and the developed glucose biosensor exhibits a wider linear glucose response compared with other polymer-based glucose biosensors ( Figure 2 A).…”

“… ( A ) Schematic diagram of poly−FA−GOx−BSA biosensing membrane synthesis, reprinted with permission [ 45 ], with permission of MDPI publications; ( B ) schematic illustration of the preparation of GO−modified carbon fibers by DR (a), AuNPs and GOD (b), reprinted with permission 51], with permission of Elsevier publications; ( C ) cyclic voltammograms were recorded at 1 mV s −1 for the optimized enzyme electrode. The enzyme electrode consisted of CNT−GOx (150 μg), Os(py)PVI (95 μg), and PEGDGE (34.2 μg), adopted from ref [ 48 ] with permission from the Elsevier publishers.…”

Direct Electrochemistry of Glucose Dehydrogenase-Functionalized Polymers on a Modified Glassy Carbon Electrode and Its Molecular Recognition of Glucose

“…To improve the assay’s accuracy and reliability, mediator-type glucose oxidase (GOD) sensors were developed and tested on electronic receptors in vitro [ 9 ]. Its enzymatic reaction, however, is highly dependent on the concentration of dissolved oxygen as an electron acceptor [ 10 , 11 , 12 ].…”