Biosensing of opioids using frog melanophores

Karlsson, Annika M.; Bjuhr, Katarina; Testorf, Martin F.; Öberg, Peter; Lerner, Ethan A.; Lundström, Ingemar; Svensson, Samuel

doi:10.1016/s0956-5663(01)00284-6

Cited by 26 publications

(13 citation statements)

References 18 publications

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“…have been described as a specific sensor for morphine (Karlsson et al, 2002). Rat basophilic leukemia mast cells in concert with fluorescent indicator dyes have been developed as a biosensor for Escherichia coli and Listeria monocytogenes (Curtis et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Conservation of the chromatophore pigment response

Dukovcic

Hutchison

Trempy

2010

J of Applied Toxicology

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Toxicant sensing technology has evolved to include biological sensors, such as cell-based biosensors, which rely on viable cells to convey a measurable physiological signal. Chromatophores are a class of pigment cells that have been investigated as cell-based biosensors. We report the characterization of Oncorhynchus tshawytscha melanophores and describe the melanophore pigment response to neurotransmitters in terms of pigment area occupied. Compared with the previously described model, Betta splendens erythrophores, O. tshawytscha melanophores responded similarly, indicating that pigment responses are biologically conserved between these two species. Additionally, melanophores responded to mercuric chloride and sodium arsenite, similar to B. splendens erythrophores, suggesting that melanophores can be used as detectors for environmental toxicants. This report highlights the potential of O. tshawytscha melanophores to be used as cell-based biosensors to address environmental toxicity, and warrants a continued investigation to strengthen this technology and its applications.

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Section: Discussionmentioning

confidence: 99%

Conservation of the chromatophore pigment response

Dukovcic

Hutchison

Trempy

2010

J of Applied Toxicology

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“…Dispersion has also been shown to be stimulated through activation of phospholipase C (PLC) which, via inositol-1,4,5-trisphosphate (IP 3 ) and diacylglycerol (DAG), induces activation of protein kinase C (PKC) and the necessary protein phosphorylations (Graminski et al, 1993;Karne et al, 1993;McClintock et al, 1996;Reilein et al, 1998). Due to their quick response (colour change) melanophores are excellent for evaluating effects of drugs acting on G-protein-coupled signalling pathways and have previously been used as biosensors, detecting opioids and formoterol in blood plasma (Karlsson et al, 2002;Filippini et al, 2003). In this study we use the melanophore system to investigate the effects of Panax ginseng on organelle transport and signalling.…”

Section: Introductionmentioning

confidence: 99%

Panax ginseng induces anterograde transport of pigment organelles in Xenopus melanophores

Eriksson

Svensson

Lundström

et al. 2008

Journal of Ethnopharmacology

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“…By using constitutive activated receptors, the simultaneous screen for agonist and inverse agonist is possible, 10 and, besides its use for drug screening, the system is also promising for optical biosensing. [11][12][13] Clonal populations of cells exhibit substantial phenotypic variation. The heterogeneity is suspected to arise from stochasticity in gene expression or by fluctuations in the amount of cellular components.…”

Section: Introductionmentioning

confidence: 99%

Addressing Variability in aXenopus laevisMelanophore Cell Line

Suska

Ibáñez

Filippini

et al. 2008

ASSAY and Drug Development Technologies

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Xenopus laevis melanophores can be used in high-throughput screens for guanine nucleotide binding protein coupled receptor ligands and have potential as biosensors. Inherent in this immortal cell line is a substantial variability, which macroscopic evaluations disregard. Here we demonstrate a systematic way to incorporate this natural variability in the evaluations. Clusters of similar cells from a sparsely seeded cell culture are examined by imaging changes in cell appearance, pigment motility, and cumulative displacements. The time evolution of the image intensity distributions of clusters upon a pigment-dispersing stimulus is used as a signature of the cell clusters, and their behaviors are classified by multivariate analysis. Conventional image subtraction procedures are used to highlight cumulative and transitory changes in the pigment dynamics, enabling characterization of multiple aspects of the cell response from a single experiment. Additionally, a simple way to accomplish standard optical density changes at the single-cell group level is shown. The present results also provide evidence that natural cell variability arising from a cell culture can enrich the diversity of responses from pigment-containing cells assays and underscore that in conventional macroscopic evaluations these aspects are overlooked and can lead to spurious results.

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Biosensing of opioids using frog melanophores

Cited by 26 publications

References 18 publications

Conservation of the chromatophore pigment response

Conservation of the chromatophore pigment response

Panax ginseng induces anterograde transport of pigment organelles in Xenopus melanophores

Addressing Variability in aXenopus laevisMelanophore Cell Line

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