Biosensing Using a Simple Resistor: The Effect of Functionalization on Sensing Devices

Saab, Marie-Belle; Ahmar, Elie Al; Estephan, Elias

doi:10.1155/2019/6291389

Cited by 3 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both electrodes obtained by the sputter deposition method showed a similar change of electrochemical parameters after 0.5 h of protein immobilization, whereas after 1 h immobilization a higher percentage change of these parameters was noted for the 20sd_AgNPs/TNT sample. High values of standard deviations in some cases may indicate incomplete saturation of the electrodes; on the other hand, because of their size, large proteins can cover the surface very unevenly, so their adsorbed amount may be random, which is reflected in the obtained results of impedance tests …”

Section: Resultsmentioning

confidence: 62%

“…Moreover, the electric charge of albumin allows its adsorption to charged surfaces. Like another serum albumin, BSA has many physiological functions, including binding and delivery of metal ions and organic molecules. , Therefore, the detection of albumin is essential. Furthermore, in this work, the impact of BSA’s immobilization time on the electrode electrochemical characteristics was also verified.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrodes Based on a Titanium Dioxide Nanotube–Spherical Silver Nanoparticle Composite for Sensing of Proteins

Nycz

Arkusz

Pijanowska

2020

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

The aim of the research was to provide electrochemical, chemical, phase, and microscopic characteristics of electrodes based on titanium dioxide nanotubes (TNTs) containing uniformly deposited, nonagglomerated spherical silver nanoparticles (AgNPs). The nanoparticles were produced with the use of electrodeposition and sputter deposition methods. This paper presents the results of research of these platforms with the use of the following techniques: electrochemical impedance spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, and scanning electron microscopy. Evaluation of the adsorption of proteinsbovine serum albumin (BSA)was carried out to establish the possibility of the use of the electrodes in a low-cost, simple detection system without surface functionalization. The research proved that the AgNP deposition facilitated the electron transfer increasing their conductivity properties as well as promoting the protein adsorption. The AgNPs/TNT electrodes showed a high selectivity to the BSA–anti-BSA complex. Half an hour of immobilization was enough to completely saturate the TNT electrodes, whereas for AgNPs/TNTs, 1 h of immobilization seemed to be not enough. The impedance parameter changes for electrodes with the AgNPs reached even about 300%. The biggest changes were noted for the platform obtained using cyclic voltammetry, so it is the best detection platform for biosensing.

show abstract

Section: Resultsmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Electrodes Based on a Titanium Dioxide Nanotube–Spherical Silver Nanoparticle Composite for Sensing of Proteins

Nycz

Arkusz

Pijanowska

2020

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…Blocking affected the resistors with a resistance above 4 Ohms and a resistor segment of less than 1 mm. Thus, the results show a decrease in resistance after BSA blocking, which corresponds with the results reported by Saab et al for simple biosensing with resistors.…”

Section: Results and Discussionmentioning

confidence: 99%

Antidelaminating, Thermally Stable, and Cost-Effective Flexible Kapton Platforms for Nitrate Sensors, Mercury Aptasensors, Protein Sensors, and p-Type Organic Thin-Film Transistors

Lin

Tsai

Díaz‐Amaya

et al. 2021

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

The inkjet printing of metal electrodes on polymer films is a desirable manufacturing process due to its simplicity but is limited by the lack of thermal stability and serious delaminating flaws in various aqueous and organic solutions. Kapton, adopted worldwide due to its superior thermal durability, allows the high-temperature sintering of nanoparticle-based metal inks. By carefully selecting inks (Ag and Au) and Kapton substrates (Kapton HN films with a thickness of 135 μm and a thermal resistance of up to 400 °C) with optimal printing parameters and simplified post-treatments (sintering), outstanding film integrity, thermal stability, and antidelaminating features were obtained in both aqueous and organic solutions without any pretreatment strategy (surface modification). These films were applied in four novel devices: a solid-state ion-selective (IS) nitrate (NO 3 − ) sensor, a single-stranded DNA (ssDNA)-based mercury (Hg 2+ ) aptasensor, a low-cost protein printed circuit board (PCB) sensor, and a long-lasting organic thin-film transistor (OTFT). The IS NO 3 − sensor displayed a linear sensitivity range between 10 −4.5 and 10 −1 M (r 2 = 0.9912), with a limit of detection of 2 ppm for NO 3 − . The Hg 2+ sensor exhibited a linear correlation (r 2 = 0.8806) between the change in the transfer resistance (R CT ) and the increasing concentration of Hg 2+ . The protein PCB sensor provided a label-free method for protein detection. Finally, the OTFT demonstrated stable performance, with mobility values in the linear (μ lin ) and saturation (μ sat ) regimes of 0.0083 ± 0.0026 and 0.0237 ± 0.0079 cm 2 V −1 S −1 , respectively, and a threshold voltage (V th ) of −6.75 ± 3.89 V.

show abstract

“…High values of standard deviations calculated for platform modification with AuNPs, may indicate that such large molecules like BSA do not cover AuNPs/TNTs uniformly, so the number of molecules adsorbed on them is very random. Hence, the measured parameters on the same samples may differ from each other [ 40 , 41 ]. For this reason, nanoparticles with larger diameter (36.7 ± 5.9 nm) produced by chronoamperometry have a higher standard deviation.…”

Section: Resultsmentioning

confidence: 99%

Comparison of Gold Nanoparticles Deposition Methods and Their Influence on Electrochemical and Adsorption Properties of Titanium Dioxide Nanotubes

2020

View full text Add to dashboard Cite

The increasing interest of attachment of gold nanoparticles (AuNPs) on titanium dioxide nanotubes (TNTs) has been devoted to obtaining tremendous properties suitable for biosensor applications. Achieving precise control of the attachment and shape of AuNPs by methods described in the literature are far from satisfactory. This work shows the comparison of physical adsorption (PA), cyclic voltammetry (CV) and chronoamperometry (CA) methods and the parameters of these methods on TNTs properties. The structural, chemical, phase and electrochemical characterizations of TNTs, Au/TNTs, AuNPs/TNTs are carried out using scanning electron microscopy (SEM), electrochemical impedance spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy. The use of PA methods does not allow the deposition of AuNPs on TNTs. CV allows easily obtaining spherical nanoparticles, for which the diameter increases from 20.3 ± 2.9 nm to 182.3 ± 51.7 nm as a concentration of tetrachloroauric acid solution increase from 0.1 mM to 10 mM. Increasing the AuNPs deposition time in the CA method increases the amount of gold, but the AuNPs diameter does not change (35.0 ± 5 nm). Importantly, the CA method also causes the dissolution of the nanotubes layer from 1000 ± 10.0 nm to 823 ± 15.3 nm. Modification of titanium dioxide nanotubes with gold nanoparticles improved the electron transfer and increased the corrosion resistance, as well as promoted the protein adsorption. Importantly, after the deposition of bovine serum albumin, an almost 5.5-fold (324%) increase in real impedance, compared to TNTs (59%) was observed. We found that the Au nanoparticles—especially those with smaller diameter—promoted the stability of bovine serum albumin binding to the TNTs platform. It confirms that the modification of TNTs with gold nanoparticles allows the development of the best platform for biosensing applications.

show abstract

Biosensing Using a Simple Resistor: The Effect of Functionalization on Sensing Devices

Cited by 3 publications

References 26 publications

Electrodes Based on a Titanium Dioxide Nanotube–Spherical Silver Nanoparticle Composite for Sensing of Proteins

Electrodes Based on a Titanium Dioxide Nanotube–Spherical Silver Nanoparticle Composite for Sensing of Proteins

Antidelaminating, Thermally Stable, and Cost-Effective Flexible Kapton Platforms for Nitrate Sensors, Mercury Aptasensors, Protein Sensors, and p-Type Organic Thin-Film Transistors

Comparison of Gold Nanoparticles Deposition Methods and Their Influence on Electrochemical and Adsorption Properties of Titanium Dioxide Nanotubes

Contact Info

Product

Resources

About