Biosensing with plasmonic nanosensors

Anker, Jeffrey N.; Hall, W. Paige; Lyandres, Olga; Shah, Nilam C.; Zhao, Jing; Duyne, Richard P. Van

doi:10.1142/9789814287005_0032

Cited by 420 publications

(417 citation statements)

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“…This nanostructure-based molecular vibrational spectroscopy can provide non-destructive and ultra-sensitive characterization down to single molecule level, comparable to single molecule fluorescence spectroscopy [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31]. However, SERS has not been adopted as a general tool due to lack of substrate (materials) generality and surface (morphology) generality.…”

mentioning

confidence: 99%

Shell‐Isolated Nanoparticle‐Enhanced Raman Spectroscopy (SHINERS)

Zhang

2014

Frontiers of Surface‐Enhanced Raman Scattering

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confidence: 99%

Shell‐Isolated Nanoparticle‐Enhanced Raman Spectroscopy (SHINERS)

Zhang

2014

Frontiers of Surface‐Enhanced Raman Scattering

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“…Out of a broad variety of techniques applied to characterize bioconjugate systems 1,3 , surface enhanced Raman spectroscopy (SERS) has emerged as a highly promising method for the detection of chemical and biological species on surfaces 8,9,10,11 . SERS employs inelastic scattering of monochromatic light by surface-immobilized biomolecules (Figure 1) allowing the capture of unique signatures corresponding to molecular vibrations.…”

Section: Introductionmentioning

confidence: 99%

“…Another important advantage of SERS is its high sensitivity. The excitation of localized surface plasmons by light interacting with noble metal nanostructures (SERS substrates) increases dramatically the intensity of Raman scattering by the analyte, allowing the detection of very small amounts of molecules, from monolayers down to the singlemolecule limit 8,9,10,11 . Finally, most biomolecules require aqueous solutions to be stable.…”

Section: Introductionmentioning

confidence: 99%

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Peters

Gutierrez-Rivera

Dew

et al. 2015

JoVE

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Fabrication and characterization of conjugate nano-biological systems interfacing metallic nanostructures on solid supports with immobilized biomolecules is reported. The entire sequence of relevant experimental steps is described, involving the fabrication of nanostructured substrates using electron beam lithography, immobilization of biomolecules on the substrates, and their characterization utilizing surface-enhanced Raman spectroscopy (SERS). Three different designs of nano-biological systems are employed, including protein A, glucose binding protein, and a dopamine binding DNA aptamer. In the latter two cases, the binding of respective ligands, D-glucose and dopamine, is also included. The three kinds of biomolecules are immobilized on nanostructured substrates by different methods, and the results of SERS imaging are reported. The capabilities of SERS to detect vibrational modes from surface-immobilized proteins, as well as to capture the protein-ligand and aptamer-ligand binding are demonstrated. The results also illustrate the influence of the surface nanostructure geometry, biomolecules immobilization strategy, Raman activity of the molecules and presence or absence of the ligand binding on the SERS spectra acquired.

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“…1,2 These fluorescence molecular rulers are fundamentally limited by light scattering in biological samples, which severely restricts the penetration depth for imaging due to the short (<100 μm) mean free path of photons in biological media. 3 As a result, the application of fluorescence-based molecular rulers is typically limited to the study of cells in vitro 4,5 or to superficial applications with intravital microscopy in vivo.…”

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confidence: 99%

Photoacoustic molecular rulers based on DNA nanostructures

Joseph

Köehler

Zuehlsdorff

et al. 2017

Preprint

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Molecular rulers that rely on the Förster resonance energy transfer (FRET) mechanism are widely used to investigate dynamic molecular processes that occur on the nanometer scale. However, the capabilities of these fluorescence molecular rulers are fundamentally limited to shallow imaging depths by light scattering in biological samples. Photoacoustic tomography (PAT) has recently emerged as a high resolution modality for in vivo imaging, coupling optical excitation with ultrasound detection. In this paper, we report the capability of PAT to probe distance-dependent FRET at centimeter depths. Using DNA nanotechnology we created several nanostructures with precisely positioned fluorophore-quencher pairs over a range of nanoscale separation distances. PAT of the DNA nanostructures showed distancedependent photoacoustic signal generation and experimentally demonstrated the ability of PAT to reveal the FRET process deep within tissue mimicking phantoms. Further, we experimentally validated these DNA nanostructures as providing a novel and biocompatible strategy to augment the intrinsic photoacoustic signal generation capabilities of small molecule fluorescent dyes.

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Biosensing with plasmonic nanosensors

Cited by 420 publications

References 0 publications

Shell‐Isolated Nanoparticle‐Enhanced Raman Spectroscopy (SHINERS)

Shell‐Isolated Nanoparticle‐Enhanced Raman Spectroscopy (SHINERS)

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Photoacoustic molecular rulers based on DNA nanostructures

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