Biosensor Applications of MAPLE Deposited Lipase

Califano, Valeria; Bloisi, F.; Aronne, Antonio; Federici, Stefania; Nasti, Libera; Depero, Laura E.; Vicari, L.

doi:10.3390/bios4040329

Cited by 14 publications

(4 citation statements)

References 42 publications

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“…In another research, the same fungal enzyme is immobilized on a mesoporous silica as a matrix for the detection of triglyceride levels (X. R. Huang et al, 2001). Moreover, the Candida rugosa strain is used as a catalyst and a biosensor for the detection of β‐hydroxyacid esters and triglycerides in blood serum (Califano et al, 2014; Mehta et al, 2017).…”

Section: Biotechnological Applicationsmentioning

confidence: 99%

Fungal lipases as biocatalysts: A promising platform in several industrial biotechnology applications

Mahfoudhi

Benmabrouk

Fendri

et al. 2022

Biotech & Bioengineering

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Many researchers have found fungi as a reliable source of lipase due to the versatility of their properties, ease of mass production, thermal stability, pH stability, broad substrate specificity, retained activity in organic solvents, and their low‐cost extraction procedure. This review paper presents an overview about the main aspects of fungal lipases screened from several types of strains as well as their use as biocatalysts. Additionally, some biochemical properties will be reported. As commonly known, lipases can be produced from animals, plants, and microorganisms. Compared to other lipases, those obtained from fungi have been found to be more productive, a fact that encouraged the massive production of most fungal lipases due to their considerable commercial importance during the past few years. This paper is concerned about some of the major characteristics that made fungal lipases desirable products in the industrial fields. Due to the enantioselective properties of fungal lipases and their ability to remain active under extreme temperature, pH, and organic solvents, enzymes are capable to synthesize esters as well as to catalyze a variety of chemical reactions that include esterification, transesterification, acidolysis and aminolysis in aqueous and nonaqueous media. Furthermore, lipases are considered to have a commercial importance for biotechnological application fields, which makes them increasingly popular in food, detergent, cosmetic, organic synthesis, and pharmaceutical domains. The biotechnological potential of lipases has made the latter a coveted choice in industries for the present and future as biocatalysts. In addition, a classification of these fungal enzymes is also highlighted in this review. Moreover, the impact of an immobilization strategy of these fungal strains to achieve higher yields and to improve their production is discussed. Finally, fungal enzymes have played a crucial role from ancient times to today in different fields using several types of biological systems, which gives them a great interest for the production of these enzymes in large amounts with low cost and easy viability to enlarge their use in many industries. Likewise, some future perspectives on lipase production will also be discussed by focusing on special cases on lipase engineering.

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Section: Biotechnological Applicationsmentioning

confidence: 99%

Fungal lipases as biocatalysts: A promising platform in several industrial biotechnology applications

Mahfoudhi

Benmabrouk

Fendri

et al. 2022

Biotech & Bioengineering

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“…Lipases, that are immobilized onto pH/oxygen electrodes in combination with glucose oxidase can function as lipid biosensors and can be used as triglyceride and blood cholesterol determinants . Especially, the biosensors with lipase immobilized through matrix assisted pulsed laser evaporation have found their application in monitoring of blood serum β‐hydroxy acid esters and triglycerides levels, environmental, and industrial components, etc . In addition, lipase immobilized on a mesoporous silica matrix is used in the making of potentiometric biosensors .…”

Section: Applications Of Lipase‐catalyzed Reactionsmentioning

confidence: 99%

“…2 Especially, the biosensors with lipase immobilized through matrix assisted pulsed laser evaporation have found their application in monitoring of blood serum bhydroxy acid esters and triglycerides levels, environmental, and industrial components, etc. 277,278 In addition, lipase immobilized on a mesoporous silica matrix is used in the making of potentiometric biosensors. 279 Recent research efforts are on towards increasing the efficacy of biosensors by the incorporation of more than one enzyme in a reaction system wherein lipases are used in conjugation with cholesterol oxidase which exhibited enhanced catalytic activity in the presence of graphene at low concentrations.…”

Section: Agrochemicalsmentioning

confidence: 99%

Recent advances on sources and industrial applications of lipases

Sarmah

Revathi

Sheelu

et al. 2017

Biotechnology Progress

301

148

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Lipases are the industrially important biocatalysts, which are envisioned to have tremendous applications in the manufacture of a wide range of products. Their unique properties such as better stability, selectivity and substrate specificity position them as the most expansively used industrial enzymes. The research on production and applications of lipases is ever growing and there exists a need to have a latest review on the research findings of lipases. The present review aims at giving the latest and broadest overall picture of research and development on lipases by including the current studies and progressions not only in the diverse industrial application fields of lipases, but also with regard to its structure, classification and sources. Also, a special emphasis has been made on the aspects such as process optimization, modeling, and design that are very critical for further scale-up and industrial implementation. The detailed tabulations provided in each section, which are prepared by the exhaustive review of current literature covering the various aspects of lipase including its production and applications along with example case studies, will serve as the comprehensive source of current advancements in lipase research. This review will be very useful for the researchers from both industry as well as academia in promoting lipolysis as the most promising approaches to intensified, greener and sustainable processes. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:5-28, 2018.

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“…Other advantages of this technique are the low consumption of materials, the control over thickness (by monitoring the deposition rate), control over surface roughness (by changing the target characteristics and number of pulses), and uniform transfer on non-planar substrates, as well the deposition of composite or multilayer films/coatings [33][34][35][36][37]. MAPLE allows the transfer of a wide range of materials, being thus successfully used for transferring different types of organic materials and polymers, such as the chemoselective polymers [38], nanoparticles [39], electro-conductive polymers [40,41], biodegradable polymers [42], thermoresponsive polymers [43], and even active proteins [44,45], as well as layers of organic materials with graded composition [39], liquid crystals [46,47], and much other more [44,48,49].…”

Section: Introductionmentioning

confidence: 99%

Induced Hydrophilicity and In Vitro Preliminary Osteoblast Response of Polyvinylidene Fluoride (PVDF) Coatings Obtained via MAPLE Deposition and Subsequent Thermal Treatment

et al. 2020

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Recent advancements in biomedicine have focused on designing novel and stable interfaces that can drive a specific cellular response toward the requirements of medical devices or implants. Among these, in recent years, electroactive polymers (i.e., polyvinylidene fluoride or PVDF) have caught the attention within the biomedical applications sector, due to their insolubility, stability in biological media, in vitro and in vivo non-toxicity, or even piezoelectric properties. However, the main disadvantage of PVDF-based bio-interfaces is related to the absence of the functional groups on the fluoropolymer and their hydrophobic character leading to a deficiency of cell adhesion and proliferation. This work was aimed at obtaining hydrophilic functional PVDF polymer coatings by using, for the first time, the one-step, matrix-assisted pulsed evaporation (MAPLE) method, testing the need of a post-deposition thermal treatment and analyzing their preliminary capacity to support MC3T3-E1 pre-osteoblast cell survival. As osteoblast cells are known to prefer rough surfaces, MAPLE deposition parameters were studied for obtaining coatings with roughness of tens to hundreds of nm, while maintaining the chemical properties similar to those of the pristine material. The in vitro studies indicated that all surfaces supported the survival of viable osteoblasts with active metabolisms, similar to the “control” sample, with no major differences regarding the thermally treated materials; this eliminates the need to use a secondary step for obtaining hydrophilic PVDF coatings. The physical-chemical characteristics of the thin films, along with the in vitro analyses, suggest that MAPLE is an adequate technique for fabricating PVDF thin films for further bio-applications.

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Biosensor Applications of MAPLE Deposited Lipase

Cited by 14 publications

References 42 publications

Fungal lipases as biocatalysts: A promising platform in several industrial biotechnology applications

Fungal lipases as biocatalysts: A promising platform in several industrial biotechnology applications

Recent advances on sources and industrial applications of lipases

Induced Hydrophilicity and In Vitro Preliminary Osteoblast Response of Polyvinylidene Fluoride (PVDF) Coatings Obtained via MAPLE Deposition and Subsequent Thermal Treatment

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