Biosensor Based on Immobilized Nitrate Reductase for the Quantification of Nitrate Ions in Dry-Cured Ham

Abstract

Manuscript Click here to download Manuscript Revised text.docx Click here to view linked References 2 Abstract 39 40 A nitrate reductase immobilized system with an oxygen electrode has been developed and 41 optimised to determine the nitrate content in dry-cured ham. The obtained amperometric 42 signal was recorded at s in the immobilised nitrate reductase sensor and the reaction 43 rates (slope) were related to the nitrate content. A linear relationship between the 44 reduction nitrate rate by action of nitrate reductase and the nitrate concentration was 45 found within the range 10-70µM (R 2 =0.9761). The immobilized enzyme showed a high 46 specificity and sensibility and was stable enough to allow the reutilization of the 47 HPLC revealed very good agreement (R 2 = 0.971). The use of this sensor may constitute 51 an interesting and valid alternative for the quantification of nitrate in dry cured ham.