Biosensor characterization of antigenic site A of foot-and-mouth disease virus presented in different vector systems

Benito, Antoni; Regenmortel, M.H.V. Van

doi:10.1111/j.1574-695x.1998.tb01155.x

Cited by 11 publications

(1 citation statement)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We previously showed that multiple insertions of a major FMDV B-cell epitope from the VP1 capsid protein near the active site of recombinant ␤-galactosidases dramatically increased the enzyme responsiveness to specific antipeptide antibodies, including sera from infected animals (4,17). In this study, we report that recombinant ␤-galactosidases accommodating one or two different peptides from the FMDV NS protein 3B per enzyme monomer can be reactivated by anti-3B monoclonal antibodies (MAbs).…”

mentioning

confidence: 95%

Discriminating Foot-and-Mouth Disease Virus-Infected and Vaccinated Animals by Use of β-Galactosidase Allosteric Biosensors

Sánchez-Aparicio

Rosas

Ferraz

et al. 2009

Clin Vaccine Immunol

View full text Add to dashboard Cite

Recombinant ␤-galactosidases accommodating one or two different peptides from the foot-and-mouth disease virus (FMDV) nonstructural protein 3B per enzyme monomer showed a drastic enzymatic activity reduction, which mainly affected proteins with double insertions. Recombinant ␤-galactosidases were enzymatically reactivated by 3B-specific murine monoclonal and rabbit polyclonal antibodies. Interestingly, these recombinant ␤-galactosidases, particularly those including one copy of each of the two 3B sequences, were efficiently reactivated by sera from infected pigs. We found reaction conditions that allowed differentiation between sera of FMDV-infected pigs, cattle, and sheep and those of naïve and conventionally vaccinated animals. These FMDV infection-specific biosensors can provide an effective and versatile alternative for the serological distinction of FMDV-infected animals.

show abstract

mentioning

confidence: 95%

Discriminating Foot-and-Mouth Disease Virus-Infected and Vaccinated Animals by Use of β-Galactosidase Allosteric Biosensors

Sánchez-Aparicio

Rosas

Ferraz

et al. 2009

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

Active concentration measurements of recombinant biomolecules using biosensor technology

1999

View full text Add to dashboard Cite

Whereas the concentration of a biomolecule simply refers to the amount of chemical substance per unit of volume, its active concentration refers to a relational parameter that has meaning only with respect to the molecule's ability to interact specifically with one particular ligand. When proteins are studied in a biological context, it is the biologically active concentration that is relevant, and not the total concentration of correctly and incorrectly folded molecules. Using a biosensor instrument the concentration of active biomolecules in a preparation can be measured by injecting the preparation at different flow rates onto a sensor chip surface presenting a high concentration of a specific ligand. The method can be used under conditions of partial mass transport limitation and does not require a pre-established standard curve. When the method was used to measure the active concentration of several recombinant proteins it was found that the active concentration was much lower than the nominal concentration determined by conventional methods. The active concentration also depended on the ligand used in the binding assay, reflecting the fact that active concentration can only be defined with respect to one specific probe. Such discrepancies in concentration values, if undetected, may lead to erroneous conclusions regarding the properties and behaviour of recombinant proteins tested in different assays.

show abstract

Survey of the 1998 optical biosensor literature

Myszka

1999

J. Mol. Recognit.

120

View full text Add to dashboard Cite

The utilization of optical biosensors to study molecular interactions continues to expand. In 1998, 384 articles relating to the use of commercial biosensors were published in 130 different journals. While significant strides in new applications and methodology were made, a majority of the biosensor literature is of rather poor quality. Basic information about experimental conditions is often not presented and many publications fail to display the experimental data, bringing into question the credibility of the results. This review provides suggestions on how to collect, analyze and report biosensor data.

show abstract

Biosensor characterization of antigenic site A of foot-and-mouth disease virus presented in different vector systems

Cited by 11 publications

References 44 publications

Discriminating Foot-and-Mouth Disease Virus-Infected and Vaccinated Animals by Use of β-Galactosidase Allosteric Biosensors

Discriminating Foot-and-Mouth Disease Virus-Infected and Vaccinated Animals by Use of β-Galactosidase Allosteric Biosensors

Active concentration measurements of recombinant biomolecules using biosensor technology

Survey of the 1998 optical biosensor literature

Contact Info

Product

Resources

About