Biosensor for the estimation of low biochemical oxygen demand

Biosensor technology for determination of BOD

“…We have developed a BOD sensor using an isolated bacterial strain of Pseudomonas putida to determine low BOD levels such as are present in river waters [23]. As a test medium we used different dilutions of artificial wastewater for calibration of the BOD sensor.…”

Biosensor for the Determination of Biochemical Oxygen Demand in Rivers

“…Many soils, muds and activated sludge samples were collected and screened as to their ability to biodegrade humic acid and lignin etc. Pseudomonas putida strain SG10 as an optimal bioelement was isolated under aerobic conditions in the limited medium (Chee et al, 1999b (APHA, 1986). The BOD 5 test method, however, requires not only many complicated procedures, including a 5-day incubation, but also experience and skill to get reproducible results.…”

The Microbial Cell Based Biosensors

“…These situations, in particular, take place in the field of environmentally oriented sensors. Thus, the analysis of wastes supposes not so much that the determination of particular pollutant as the assessment of a group of compounds or total content of organics indirectly determined through the BOD index (Chee et al, 1999;Kim & Park, 2001). In these conditions the use of sensors sensitive to a wide range of substances is essentially efficient (Lehmann et al, 1999;Suriyawattanakul et al, 2002).…”

“…Therefore, pure bacterial and fungal cultures with broad substrate specificity are used in BOD sensors in parallel with active sludge and other microbial mixtures. The examples of such sensors are descirbed in the works (Yang et al, 1997;Kim & Park, 2001;Chee et al, 1999) etc. The list of the microorganisms most frequently used in BOD sensors includes T. cutaneum, Arxula adeninovorans, E. coli, Bacillus and Pseudomonas species and other microorganisms with broad specificity.…”