Biosensors: A novel approach to and recent discovery in detection of cytokines

Mobed, Ahmad; Shakouri, Seyed Kazem

doi:10.1016/j.cyto.2020.155272

Cited by 19 publications

(10 citation statements)

References 57 publications

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“…By integrating with nanotechnology, biosensors as the analytical devices for the detection of analytes that combines biological components with a physicochemical detector, has demonstrated great potential for sensing. Such cytokines biosensors [124] can rely on fluorescence or electrochemical signal readouts to quantify intracellular and extracellular cytokines in non-real-time [39,125] or in real-time. [31,126] They can also use other transducing elements.…”

Section: Biosensors For Cytokines Detectionmentioning

confidence: 99%

Cytokines: From Clinical Significance to Quantification

et al. 2021

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Cytokines are critical mediators that oversee and regulate immune and inflammatory responses via complex networks and serve as biomarkers for many diseases. Quantification of cytokines has significant value in both clinical medicine and biology as the levels provide insights into physiological and pathological processes and can be used to aid diagnosis and treatment. Cytokines and their clinical significance are introduced from the perspective of their pro‐ and anti‐inflammatory effects. Factors affecting cytokines quantification in biological fluids, native levels in different body fluids, sample processing and storage conditions, sensitivity to freeze‐thaw, and soluble cytokine receptors are discussed. In addition, recent advances in in vitro and in vivo assays, biosensors based on different signal outputs and intracellular to extracellular protein expression are summarized. Various quantification platforms for high‐sensitivity and reliable measurement of cytokines in different scenarios are discussed, and commercially available cytokine assays are compared. A discussion of challenges in the development and advancement of technologies for cytokine quantification that aim to achieve real‐time multiplex cytokine analysis for point‐of‐care situations applicable for both biomedical research and clinical practice are discussed.

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Section: Biosensors For Cytokines Detectionmentioning

confidence: 99%

Cytokines: From Clinical Significance to Quantification

et al. 2021

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“…Detection of cytokines in biological samples requires the use of analytical methods characterized by high sensitivity and specificity, taking into account the very low levels of these biomarkers and the complex matrix in which they are found. 66,67 Conventional detection methods were developed starting from the immunological methods such as enzymelinked immunosorbent assay (ELISA), 67,68 hyphenated chromatographic methods combining high-performance liquid chromatography with diverse detection methods: mass spectrometry (MS), UV-VIS spectrophotometry, and electrochemical methods, 69 as well as several spectrophotometric techniques 70 and DNA-based methods. 69 The special requirements for analytical parameters that are suitable for the detection of these biomarkers have led researchers to look for alternative methods.…”

Section: Analytical Methods Applied For Cytokines Determination In Bi...mentioning

confidence: 99%

Unrevealing the connection between real sample analysis and analytical method. The case of cytokines

Irimes

Tertiş

Oprean

et al. 2023

Medicinal Research Reviews

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Cytokines are compounds that belong to a special class of signaling biomolecules that are responsible for several functions in the human body, being involved in cell growth, inflammatory, and neoplastic processes. Thus, they represent valuable biomarkers for diagnosing and drug therapy monitoring certain medical conditions. Because cytokines are secreted in the human body, they can be detected in both conventional samples, such as blood or urine, but also in samples less used in medical practice such as sweat or saliva. As the importance of cytokines was identified, various analytical methods for their determination in biological fluids were reported. The gold standard in cytokine detection is considered the enzyme‐linked immunosorbent assay method and the most recent ones have been considered and compared in this study. It is known that the conventional methods are accompanied by a few disadvantages that new methods of analysis, especially electrochemical sensors, are trying to overcome. Electrochemical sensors proved to be suited for the elaboration of integrated, portable, and wearable sensing devices, which could also facilitate cytokines determination in medical practice.

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“…Although relatively inexpensive and amenable to multiplexing, ELISA-based assays are not suitable for POC usage as it typically takes hours to prepare, process, and read the samples. It also requires a large sample size and relies on bulky equipment that is not suitable for POC applications [16,31]. With the limitations of conventional assays and the need for critical diagnostic cytokine measurements, many biosensor-based technologies have been developed as a simple, sensitive and inexpensive tool for cytokine detection.…”

Section: Biosensor (Lab-on-a-chip) Concept For Cytokines Detectionmentioning

confidence: 99%

Advances in Nanotechnology-Based Biosensing of Immunoregulatory Cytokines

2021

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Cytokines are a large group of small proteins secreted by immune and non-immune cells in response to external stimuli. Much attention has been given to the application of cytokines’ detection in early disease diagnosis/monitoring and therapeutic response assessment. To date, a wide range of assays are available for cytokines detection. However, in specific applications, multiplexed or continuous measurements of cytokines with wearable biosensing devices are highly desirable. For such efforts, various nanomaterials have been extensively investigated due to their extraordinary properties, such as high surface area and controllable particle size and shape, which leads to their tunable optical emission, electrical, and magnetic properties. Different types of nanomaterials such as noble metal, metal oxide, and carbon nanoparticles have been explored for various biosensing applications. Advances in nanomaterial synthesis and device development have led to significant progress in pushing the limit of cytokine detection. This article reviews currently used methods for cytokines detection and new nanotechnology-based biosensors for ultrasensitive cytokine detection.

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Biosensors: A novel approach to and recent discovery in detection of cytokines

Cited by 19 publications

References 57 publications

Cytokines: From Clinical Significance to Quantification

Cytokines: From Clinical Significance to Quantification

Unrevealing the connection between real sample analysis and analytical method. The case of cytokines

Advances in Nanotechnology-Based Biosensing of Immunoregulatory Cytokines

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