Biosensors based on oxidases immobilized in various conducting polymers

Trojanowicz, Marek; Geschke, Oliver; Krawczyk, Tadeusz Krawczyñski vel; Cammann, Karl

doi:10.1016/0925-4005(95)01724-0

Cited by 105 publications

(57 citation statements)

References 15 publications

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“…Wang and Chen [15] have recently prepared enzyme microelectrode array strips for estimation of glucose and lactate. Further, Trojanowicz et al [16] have electrochemically immobilized lactate oxidase in polypyrrole ®lms. Ramanathan et al [17] have immobilized LDH on tetraethylorthosilicate (TEOS) derived sol-gel ®lms by physical adsorption and entrapment techniques.…”

Section: Introductionmentioning

confidence: 98%

Immobilization of Lactate Dehydrogenase on Electrochemically Prepared Polyaniline Films

Gerard¹,

Ramanathan

Chaubey

et al. 1999

Electroanalysis

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Section: Introductionmentioning

confidence: 98%

Immobilization of Lactate Dehydrogenase on Electrochemically Prepared Polyaniline Films

Gerard¹,

Ramanathan

Chaubey

et al. 1999

Electroanalysis

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“…The hydrogen peroxide formed in the reaction of oxidation can be quantified by electrochemical methods and its amount is directly related with the sugar quantity. The immovilized enzymes in polypirrol have been carried out by several methods such as physical adsorption, covalent union, entrapment and electrochemical doping [2][3][4][5][6][7][8][9] . The entrapment method involves to capture the enzyme during the polymer electrosynthesis process, from a monomeric solution that contains the enzyme.…”

Section: Introductionmentioning

confidence: 99%

Biosensors' Preparation to Be Used for Entrapment Method With Copolymers Polyaniline Derivatives

Sánchez

Isla

Bustos

et al. 2010

J. Chil. Chem. Soc.

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The entrapment method was used to prepare electrochemical biosensors. Using controlled potential method, copolymers were synthesized between aniline y sulfonated-anilines in aqueous media at pH 4.9 on Platinum disc. The copolymers were characterized by elemental analysis and spectroscopy. Glucose-oxidase was entrapped in the electropolymerization process, at pH 4.9 in aqueous media. The copolymers modified electrodes and copolymer/enzyme were studied as working electrodes for the oxidation of different H 2 O 2 concentrations and indirect D-glucose quantification at pH 7, using controlled potential and the cyclic voltammetry methods (CV) correspondingly. A linear relationship between the hydrogen peroxide (or D-glucose) concentration and the electrochemical response was obtained at pH 7. The modified-electrodes displayed a fast time of current response, limited by the cycle numbers of the cyclic voltammetry, 232 s, and enough stability to allow for the measurements to be taken after several days.

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“…The electrochemical devices made by different conducting polymers entrapping recognized biomolecules have been reported extensively (Habermuller & Schuhmann, 1998;Rahman et al, 2004;Selampinar et al, 1997), because their major advantages (Cosnier, 1999) are that polymer film can be polymerized with immobilizing enzyme in one step, and the film thickness can be controlled easily by adjusting capacity. The most common polymers used as matrixes to entrap enzyme are polyaniline (Borole et al, 2004), polythiophene, polypyrrole and its derivatives (Trojanowicz et al, 1995). In addition to enzymes, the mediators should be immobilized in the same matrix for biosensors or biofuel cells.…”

Section: The Immobilization Of Enzymes and Mediatorsmentioning

confidence: 99%