Polyaniline based nanocomposite graphite paste electrode modified with rice husk (PANI/Gr/RH) and without rice husk (PANI/Grph) to compare the amperometric response caused by the addition of rice husk in conventional sensor. Organized materials were characterized by analytical techniques such as UV-visible, XRD and FE-SEM analyses. The performance of the developed sensor was evaluated and the obtained urea biosensor exhibited shorter response time (3 s), wider range 1 × 10 -9 to 9 × 10 -9 M and the detection limit were found to be 1 × 10 -9. About 90 % of the enzyme activity is retained for about 40 days. Modified rice husk sensor gives better result than sensor without rice husk.