Biosorption of heavy metals by lactic acid bacteria and identification of mercury binding protein

Kinoshita, Hideki; Sohma, Yui; Ohtake, Fumika; Ishida, Masaharu; Kawai, Yasushi; Kitazawa, Haruki; Saito, Tadao; Kimura, Kazuhiko

doi:10.1016/j.resmic.2013.04.004

Cited by 122 publications

(81 citation statements)

References 31 publications

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“…In this regard, the isolate could be of great use in comparison to other reported strains (Noghabi et al, 2007;Francois et al, 2012;Kinoshita et al, 2013) possessing mercury biosorption capacity.…”

Section: Discussionmentioning

confidence: 93%

“…There are many reports of mercury-resistant bacteria having biosorption capabilities. Many species of Pseudomonas, lactic acid bacteria (LAB), Bacillus, and Lysinibacillus have been reported from environmental conditions to produce EPS for biosorption of mercury (Noghabi et al, 2007;De et al, 2008;Francois et al, 2012;Kinoshita et al, 2013). Microbes for biosorption of mercury were constructed using binding and transport proteins from the mer operon, e.g., the periplasmic binding protein MerP, and the membrane-bound transport protein MerT, which have a very high affinity for Hg(II).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mercury Pollution and Bioremediation—A Case Study on Biosorption by a Mercury-Resistant Marine Bacterium

Dash

Das

2014

Microbial Biodegradation and Bioremediation

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Mercury Pollution and Bioremediation—A Case Study on Biosorption by a Mercury-Resistant Marine Bacterium

Dash

Das

2014

Microbial Biodegradation and Bioremediation

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“…Some strains had only a few or no proteins in PBS extracts. In our previous study, GAPDH enzymatic activity was measured in the PBS extracts of 30 lactobacilli isolated from human intestinal tissues and was detected in 21 out of 30 samples from 12-hr cultures and in all samples at 18 hr [34]. This suggests that in moonlighting proteins, such as GAPDH, accumulation is growth-time dependent.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we reported that GAPDH expressed on the bacterial cell surfaces of Lactobacillus plantarum LA 318 isolated from human intestinal tissue (transverse colon) [32] binds to human colonic mucin [28] and to the A- and B-type antigens of the ABO blood type group [33]. GAPDH is universally expressed on the cell surfaces of many lactobacilli [34]. According to Antikainen et al [30], GAPDH and enolase bind to lipoteichoic acid on the bacterial cell surface by ionic bonds.…”

Section: Introductionmentioning

confidence: 99%

Isolation of lactic acid bacteria bound to the porcine intestinal mucosa and an analysis of their moonlighting adhesins

Kinoshita

Ohuchi

Arakawa

et al. 2016

Bioscience of Microbiota, Food and Health

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The adhesion of lactic acid bacteria (LAB) to the intestinal mucosa is one of the criteria in selecting for probiotics. Eighteen LAB were isolated from porcine intestinal mucin (PIM): ten strains of Lactobacillus, six strains of Weissella, and two strains of Streptococcus. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for phosphate-buffered saline (PBS) extracts from the LAB, many bands were detected in half of the samples, while a few and/or no clear bands were detected in the other half. All six of the selected LAB showed adhesion to PIM. L. johnsonii MYU 214 and MYU 221 showed adhesion at more than 10%. W. viridescens MYU 208, L. reuteri MYU 213, L. mucosae MYU 225, and L. agilis MYU 227 showed medium levels of adhesion at 5.9–8.3%. In a comprehensive analysis for the adhesins in the PBS extracts using a receptor overlay analysis, many moonlighting proteins were detected and identified as candidates for adhesins: GroEL, enolase, and elongation factor Tu in MYU 208; peptidase C1, enolase, formyl-CoA transferase, phosphoglyceromutase, triosephosphate isomerase, and phosphofructokinase in MYU 221; and DnaK, enolase, and phosphoglycerate kinase in MYU 227. These proteins in the PBS extracts, which included such things as molecular chaperones and glycolytic enzymes, may play important roles as adhesins.

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“…Several mechanisms have been described for heavy metal interaction with bacteria, including specific uptake (bioaccumulation) and biosorption, a metabolically independent process based on surface adsorption, ionic interactions, chelation or microprecipitation . Studies on heavy metal(oid) sequestration by LAB have been carried out mainly with Cd(II) and Pb(II), but with also Cr(VI), Cu(II), Al(III) and As(III)/As(V) . However, despite the wide distribution of mercury and its importance as a major contaminant in food, binding of mercurial compounds by LAB has received very little attention and, as far as we know, only one report is available in the literature where the capacity to sequester Hg(II) by LAB has been examined .…”

Section: Introductionmentioning

confidence: 99%

Characterization of the binding capacity of mercurial species in Lactobacillus strains

et al. 2017

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BACKGROUND Metal sequestration by bacteria has been proposed as a strategy to counteract metal contamination in foodstuffs. Lactobacilli can interact with metals, although studies with important foodborne metals such as inorganic [Hg(II)] or organic (CH3Hg) mercury are lacking. Lactobacilli were evaluated for their potential to bind these contaminants and the nature of the interaction was assessed by the use of metal competitors, chemical and enzymatical treatments, and mutants affected in the cell wall structure. RESULTS Lactobacillus strains efficiently bound Hg(II) and CH3Hg. Mercury binding by Lactobacillus casei BL23 was independent of cell viability. In BL23, both forms of mercury were cell wall bound. Their interaction was not inhibited by cations and it was resistant to chelating agents and protein digestion. Lactobacillus casei mutants affected in genes involved in the modulation of the negative charge of the cell wall anionic polymer lipoteichoic acid showed increased mercury biosorption. In these mutants, mercury toxicity was enhanced compared to wild‐type bacteria. These data suggest that lipoteichoic acid itself or the physicochemical characteristics that it confers to the cell wall play a major role in mercury complexation. CONCLUSION This is the first example of the biosorption of Hg(II) and CH3Hg in lactobacilli and it represents a first step towards their possible use as agents for diminishing mercury bioaccessibility from food at the gastrointestinal tract. © 2017 Society of Chemical Industry

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Biosorption of heavy metals by lactic acid bacteria and identification of mercury binding protein

Cited by 122 publications

References 31 publications

Mercury Pollution and Bioremediation—A Case Study on Biosorption by a Mercury-Resistant Marine Bacterium

Mercury Pollution and Bioremediation—A Case Study on Biosorption by a Mercury-Resistant Marine Bacterium

Isolation of lactic acid bacteria bound to the porcine intestinal mucosa and an analysis of their moonlighting adhesins

Characterization of the binding capacity of mercurial species in Lactobacillus strains

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