Biospeckle Technique for Monitoring Bacterial Colony Growth with Minimal Photo-Exposure Time Associated

Ramírez-Miquet, Evelio E.; Darias, J. G.; Otero, Isabel Diz; Rodríguez, Dania; Murialdo, Silvia Elena; Rabal, H.; Trivi, Marcelo

doi:10.1007/978-3-319-13117-7_81

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…In our previous paper, 8 we presented an analysis of the diameter of the active zone obtained with a GD processing of biospeckle images of E. coli. The analysis of the diameter of growth versus time correlated to the regime of illumination yielded a graph with separated error bars that necessitated a statistical analysis to conclude further if continuous and instantaneous exposure affected the bacterial growth kinetics.…”

Section: Resultsmentioning

confidence: 99%

“…6,7 Recently, the effects of continuous and instantaneous laser exposure and intensity on the spreading rate of bacterial colonies and yeast were explored. 8,9 Bacterial spreading rate is a key parameter for quality control of possible contaminated samples. 10 An optimal image processing allowing a rapid diagnosis is crucial in online assessment of bacterial colonization in biofilms, water, or food.…”

Section: Introductionmentioning

confidence: 99%

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Real time and online dynamic speckle assessment of growing bacteria using the method of motion history image

et al. 2016

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This paper reports the application of the motion history image (MHI) method for biospeckle processing of a bacterial growth. The method avoids the complexity as well as the large computation in sequence-matching-based methods and detects whether the speckle structure has changed or not. Encouraging experimental results on the real-time evolution of the growing bacteria during 12 h demonstrate the effectiveness of the proposed method. The MHI presented an online result without loss of resolution and definition. In turn, the MHI also presented the ability to provide a close answer to the traditional offline method of generalized differences.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Real time and online dynamic speckle assessment of growing bacteria using the method of motion history image

et al. 2016

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“…Rather than investigating the static features of scattering patterns, the approaches based on the time variations of the biospeckle study the temporal decorrelation occurring as a consequence of biological activity inside the FoV [57][58][59][60][61][62]. In this type of analysis, we can consider U i = U i (t), and, in turn, I = I(x,y;t), where x and y are the spatial variables and t denotes the time variable.…”

Section: Biospeckle Analysismentioning

confidence: 99%

Biospeckle Analysis and Biofilm Electrostatic Tests, Two Useful Methods in Microbiology

Oleandro

Grilli

Rega

et al. 2021

Applied Microbiology

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The development of more sensitive methodologies, capable of quickly detecting and monitoring a microbial population present in a specific biological matrix, as well as performing to allow for the study of all its metabolic changes (e.g., during the formation of biofilm) to occur, is an essential requirement for both well-being and the food industry. Two techniques, in particular, have gained the attention of scientists: The first is “biospeckle”, an optical technique representing an innovative tool for applications in food quality, food safety, and nutraceuticals. With this technique, we can quickly evaluate and monitor the presence of bacteria (or their proliferation) in a solid or liquid biological matrix. In addition, the technique is helpful in quantifying and optimizing the correct storage time of the pro-biotics, if they are entrapped in matrices such as alginate and follow their survival rate in simulated gastro-intestinal conditions. A second technique with great chances is the “biofilm electrostatic test” (BET). BET undoubtedly represents a fast, simple, and highly reproducible tool suitable for admitting the evaluation of the in vitro bacterial capacity in order to adhere through an electrostatic interaction with a pyro-electrified carrier after only 2 h of incubation. BET could represent the way for a quick and standardized evaluation of bacterial resistance among biofilm-producing microorganisms through a fast evaluation of the potential presence of the biofilm.

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“…Therefore, the biospeckle laser is an imaging technique widely used to monitor biological activities in different research domains such as medicine or agriculture [37,38]. With a high sensitivity to follow very tiny movements in biological tissues, this technique is well-adapted for microscopic characterization of biological samples and phenomena such as bacterial spreading [39] or for the detection of drug action on parasites [40]. In plant science, at the seed scale, biospeckle imaging is especially used for the prediction of seed viability or the early detection of pathogen infestations [41,42].…”

Section: D Biospeckle Imaging Of Seed Imbibition Out Of Soilmentioning

confidence: 99%

Evaluation of 3D/2D Imaging and Image Processing Techniques for the Monitoring of Seed Imbibition

et al. 2018

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Seed imbibition is a very important process in plant biology by which, thanks to a simple water income, a dry seed may turn into a developing organism. In natural conditions, this process occurs in the soil, e.g., with difficult access for a direct observation. Monitoring the seed imbibition with non-invasive imaging techniques is therefore an important and possibly challenging task if one tries to perform it in natural conditions. In this report, we describe a set of four different imaging techniques that enable to addressing this task either in 3D or in 2D. For each technique, the following items are proposed. A detailed experimental protocol is provided to acquire images of the imbibition process. With the illustration of real data, the significance of the physical quantities measured in terms of their relation to the income of water in the seed is presented. Complete image analysis pipelines are then proposed to extract dynamic information on the imbibition process from such monitoring experiments. A final discussion compares the advantages and current limitations of each technique in addition to elements concerning the associated throughput and cost. These are criteria especially relevant in the field of plant phenotyping where large populations of plants are imaged to produce quantitatively significative traits after image processing.

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Biospeckle Technique for Monitoring Bacterial Colony Growth with Minimal Photo-Exposure Time Associated

Cited by 9 publications

References 21 publications

Real time and online dynamic speckle assessment of growing bacteria using the method of motion history image

Real time and online dynamic speckle assessment of growing bacteria using the method of motion history image

Biospeckle Analysis and Biofilm Electrostatic Tests, Two Useful Methods in Microbiology

Evaluation of 3D/2D Imaging and Image Processing Techniques for the Monitoring of Seed Imbibition

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