Biostimulant and biopesticide potential of microalgae growing in piggery wastewater

Ferreira, Alice; Melkonyan, L. H.; Carapinha, Sofia de Sousa Sapateiro Claré; Ribeiro, Belina; Figueiredo, Daniel; Avetisova, Gayane; Gouveia, Luísa

doi:10.1016/j.envadv.2021.100062

Cited by 90 publications

(42 citation statements)

References 41 publications

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“…However, the ammonium levels in this pretreated effluent were still higher than the general limit of toxicity for microalgae (around 150 mg•L −1 ) [36]. Nonetheless, a dilution of at least 1:2 could be enough to overcome this limitation, which is far better than the previous dilution factors used by Ferreira et al (2021) [28] and the literature in general (e.g., García et al, 2017 [2]; Godos et al, 2010 [37]; Molinuevo-Salces et al, 2016 [38]).…”

Section: Ammonia Stripping (As)mentioning

confidence: 70%

“…The microalga tested was Tetradesmus obliquus (ACOI 204/07, ACOI Culture Collection, Coimbra University, Portugal) that was previously shown to grow more efficiently on diluted (1/20) piggery effluent [28]. T. obliquus was inoculated in the different undiluted pretreated effluents at an optical density (OD 540 nm ) of 0.4 in 50 mL Erlenmeyer flasks with a working volume of 20 mL.…”

Section: Microalga Cultivationmentioning

confidence: 99%

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Exploring Different Pretreatment Methodologies for Allowing Microalgae Growth in Undiluted Piggery Wastewater

et al. 2022

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The overapplication of manure on agricultural soils leads to nitrogen and phosphorus discharge into the aquatic environment, resulting in serious eutrophication problems and decreased water quality. Piggery wastewater (PWW) can be treated by microalgae to recycle nutrients, but the toxic levels of ammonia and organic matter hinder their growth. Fresh water is usually used to dilute PWW, but it is a scarce resource. The implementation of a pretreatment step before microalgae-based treatment could make PWW suitable for microalgae growth. Electrocoagulation, ammonia stripping, photo-Fenton, and constructed wetlands were evaluated as pretreatment methods to reduce ammonia, chemical oxygen demand (COD), color, and total suspended solids. Moreover, the pretreated PWWs were tested to grow the microalga Tetradesmus obliquus. Photo-Fenton showed the best results among the other pretreatments, achieving removal efficiencies above 90%, except for ammonia. This resulted in T. obliquus being capable of growing on undiluted PWW, even at higher ammonia levels, achieving similar biomass productivity to synthetic medium (66.4 ± 17.8 mg·L−1·day−1 and 60.1 ± 10.4 mg·L−1·day−1, respectively) almost doubling with pH control (116.5 mg·L−1·day−1). Thus, this pretreatment seems to be the most promising one to incorporate into microalgae-based treatment systems and must be further explored.

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Section: Ammonia Stripping (As)mentioning

confidence: 70%

Section: Microalga Cultivationmentioning

confidence: 99%

Exploring Different Pretreatment Methodologies for Allowing Microalgae Growth in Undiluted Piggery Wastewater

et al. 2022

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“…However, biomass productivity was very different in each case. Mennaa et al [12] and Ferreira et al [23] reported, however, that S. obliquus (Tetradesmus obliquus) had the best performance in nutrient removal and biomass productivity.…”

Section: Composition Of Urban Wastewater and Treatment Alternatives Using Microalgaementioning

confidence: 99%

Microalgal Systems for Wastewater Treatment: Technological Trends and Challenges towards Waste Recovery

et al. 2021

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Wastewater (WW) treatment using microalgae has become a growing trend due the economic and environmental benefits of the process. As microalgae need CO2, nitrogen, and phosphorus to grow, they remove these potential pollutants from wastewaters, making them able to replace energetically expensive treatment steps in conventional WW treatment. Unlike traditional sludge, biomass can be used to produce biofuels, biofertilizers, high value chemicals, and even next-generation growth media for “organically” grown microalgal biomass targeting zero-waste policies and contributing to a more sustainable circular bioeconomy. The main challenge in this technology is the techno-economic feasibility of the system. Alternatives such as the isolation of novel strains, the use of native consortia, and the design of new bioreactors have been studied to overcome this and aid the scale-up of microalgal systems. This review focuses on the treatment of urban, industrial, and agricultural wastewaters by microalgae and their ability to not only remove, but also promote the reuse, of those pollutants. Opportunities and future prospects are discussed, including the upgrading of the produced biomass into valuable compounds, mainly biofuels.

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“…It is still debatable the need for cell disruption to exploit the benefits of microalgae as biofertilisers/biostimulants since some works such as Navarro-López et al (2020) [ 22 ] and Ferreira et al (2021) [ 23 ] showed increments on the germination index (GI) with microalgae added to seeds without any pre-treatment. Figueiredo et al (2022) [ 24 ] also showed the possibility of using mineral enriched whole-cell microalgal biomass to fulfil plants’ nutrient requirements in replacement of chemical sources.…”

Section: Introductionmentioning

confidence: 99%

Impact of High-Pressure Homogenization on the Cell Integrity of Tetradesmus obliquus and Seed Germination

et al. 2022

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Microalgae have almost unlimited applications due to their versatility and robustness to grow in different environmental conditions, their biodiversity and variety of valuable bioactive compounds. Wastewater can be used as a low-cost and readily available medium for microalgae, while the latter removes the pollutants to produce clean water. Nevertheless, since the most valuable metabolites are mainly located inside the microalga cell, their release implies rupturing the cell wall. In this study, Tetradesmus obliquus grown in 5% piggery effluent was disrupted using high-pressure homogenization (HPH). Effects of HPH pressure (100, 300, and 600 bar) and cycles (1, 2 and 3) were tested on the membrane integrity and evaluated using flow cytometry and microscopy. In addition, wheat seed germination trials were carried out using the biomass at different conditions. Increased HPH pressure or number of cycles led to more cell disruption (75% at 600 bar and 3 cycles). However, the highest increase in wheat germination and growth (40–45%) was observed at the lowest pressure (100 bar), where only 46% of the microalga cells were permeabilised, but not disrupted. Non-treated T. obliquus cultures also revealed an enhancing effect on root and shoot length (up to 40%). The filtrate of the initial culture also promoted shoot development compared to water (21%), reinforcing the full use of all the process fractions. Thus, piggery wastewater can be used to produce microalgae biomass, and mild HPH conditions can promote cell permeabilization to release sufficient amounts of bioactive compounds with the ability to enhance plant germination and growth, converting an economic and environmental concern into environmentally sustainable applications.

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Biostimulant and biopesticide potential of microalgae growing in piggery wastewater

Cited by 90 publications

References 41 publications

Exploring Different Pretreatment Methodologies for Allowing Microalgae Growth in Undiluted Piggery Wastewater

Exploring Different Pretreatment Methodologies for Allowing Microalgae Growth in Undiluted Piggery Wastewater

Microalgal Systems for Wastewater Treatment: Technological Trends and Challenges towards Waste Recovery

Impact of High-Pressure Homogenization on the Cell Integrity of Tetradesmus obliquus and Seed Germination

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