Biostimulation of biogas producing microcosm for enhancing oil recovery in low-permeability oil reservoir

Dong, Huaimin; Zhang, Zhongzhi; He, Youwei; Luo, Yijing; Xia, Wenjie; Sun, Shanshan; Zhang, Guangqing; Zhang, Z Y; Gao, De Li

doi:10.1039/c5ra18089a

Cited by 8 publications

(5 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, a large amount of microbial populations have been isolated or detected in oil reservoirs worldwide (Lenchi et al, 2013; Al-Sayegh et al, 2015; Gao et al, 2016; Okoro et al, 2016; Su et al, 2018). These microorganisms are versatile: some species can degrade crude oil, some can produce biosurfactants (Liu et al, 2015; Cui et al, 2017; Qi et al, 2018), biopolymers (Bi et al, 2016; Xia et al, 2018; Zhao et al, 2018), or biogases (Dong et al, 2015; Zhu et al, 2015), and have been used to mobilize and recover residual oil from oil-bearing rocks (Youssef et al, 2009; Wang et al, 2014; Yi et al, 2018). In addition, nitrate can be used to reduce formation of hydrogen sulfide produced by sulfate-reducing bacteria (SRB) based on the competitive inhibition of nitrate-reducing bacteria on SRB (Gieg et al, 2011; Gassara et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Composition of Bacterial and Archaeal Communities in an Alkali-Surfactant-Polyacrylamide-Flooded Oil Reservoir and the Responses of Microcosms to Nutrients

Gao

Tan

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

The microbial communities in alkali-surfactant-polyacrylamide-flooded (ASP-flooded) oil reservoirs have rarely been investigated compared to those in water-flooded oil reservoirs. Here, the bacterial and archaeal communities in an ASP-flooded reservoir and the adjacent water-flooded block, and responses of the microbial communities in microcosms to nutrients were investigated by 16S rRNA gene sequencing and cultivation. Compared with the water-flooded block, both the bacterial and archaeal communities inhabiting the ASP-flooded block had lower Sobs indices (91:232 and 34:55, respectively), lower Shannon indices (1.296:2.256 and 0.845:1.627, respectively) and higher Simpson indices (0.391:0.248 and 0.678:0.315, respectively). Halomonas (58.4–82.1%) and Anoxynatronum (14.5–18.2%) predominated in the ASP-flooded production wells, and were less than 0.05% in the bacterial communities of the adjacent water-flooded production wells, which were dominated by Pseudomonas and Thauera. Methanobacterium accounted for 65.0–94.5% of the archaeal communities inhabiting the ASP-flooded production wells, and Methanosaeta (36.7–94.5%) dominated the adjacent water-flooded production wells. After nutrients stimulation, the quantity of cultivable microorganisms increased from 103/mL to 107/mL. Community analysis indicated that the relative abundances of some species that belonged to Halomonas and Pseudomonas obviously increased, yet there were no oil emulsification or dispersion and changes of surface tension of the water-oil mixture. In addition, 6 alkali-tolerating strains showing 98% similarity of 16S rRNA genes with those of Halomonas alkalicola and Halomonas desiderata and 2 strains with 99% similarity with Pseudomonas stutzeri gene were isolated from the nutrients stimulated brines. In summary, this study indicated that Halomonas, Anoxynatronum, and Methanobacterium were dominant populations in the ASP-flooded reservoir, the extreme environment decreased microbial diversity, and restricted microbial growth and metabolisms.

show abstract

Section: Introductionmentioning

confidence: 99%

Composition of Bacterial and Archaeal Communities in an Alkali-Surfactant-Polyacrylamide-Flooded Oil Reservoir and the Responses of Microcosms to Nutrients

Gao

Tan

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…It was used to evaluate the application potential of the exogenous surfactant-producing B. subtilis in facilitating the IMEOR process. Previous investigations have shown that the stimulation of indigenous microorganisms enhances oil recovery by 3.7–9.14 % ( Bao et al, 2009 ; Dong et al, 2015 ), and by 4.89–24 % in core-flooding tests with surfactant-producing bacteria and their metabolites ( She et al, 2011 ; Castorena-Cortes et al, 2012 ; Xia et al, 2012 ; Gudina et al, 2013 ; Al-Wahaibi et al, 2014 ; Arora et al, 2014 ; Song et al, 2015 ). In this study, the brines with nutrients and fermentation broth of B. subtilis M15-10-1 increased the oil displacement efficiency by 16.71%, which is higher than the 7.59% with nutrient injection only.…”

Section: Discussionmentioning

confidence: 99%

“…With the increasing global energy demand and depletion of oil reserves, oil recovery by microbial flooding is currently under intensive development, and has been shown to be economically feasible by laboratory and field trials ( Belyaev et al, 1998 ; Gao and Zekri, 2011 ; Xiao et al, 2013 ; Li et al, 2014 ; Shibulal et al, 2014 ; Dong et al, 2015 ; You et al, 2015 ). This technique is generally classified into exogenous and indigenous microbial enhanced oil recovery (MEOR).…”

Section: Introductionmentioning

confidence: 99%

An Exogenous Surfactant-Producing Bacillus subtilis Facilitates Indigenous Microbial Enhanced Oil Recovery

Gao

et al. 2016

Front. Microbiol.

View full text Add to dashboard Cite

This study used an exogenous lipopeptide-producing Bacillus subtilis to strengthen the indigenous microbial enhanced oil recovery (IMEOR) process in a water-flooded reservoir in the laboratory. The microbial processes and driving mechanisms were investigated in terms of the changes in oil properties and the interplay between the exogenous B. subtilis and indigenous microbial populations. The exogenous B. subtilis is a lipopeptide producer, with a short growth cycle and no oil-degrading ability. The B. subtilis facilitates the IMEOR process through improving oil emulsification and accelerating microbial growth with oil as the carbon source. Microbial community studies using quantitative PCR and high-throughput sequencing revealed that the exogenous B. subtilis could live together with reservoir microbial populations, and did not exert an observable inhibitory effect on the indigenous microbial populations during nutrient stimulation. Core-flooding tests showed that the combined exogenous and indigenous microbial flooding increased oil displacement efficiency by 16.71%, compared with 7.59% in the control where only nutrients were added, demonstrating the application potential in enhanced oil recovery in water-flooded reservoirs, in particular, for reservoirs where IMEOR treatment cannot effectively improve oil recovery.

show abstract

“…The hypervariable V2–V3 region of the bacterial 16S rRNA gene was amplified with the universal primer set 104F (50-GGCGVACGGGTGAGTAA-30) and 530R (50-CCGCNGCNGCTGGCAC-30) in a 50 μL PCR mixture containing 25 μL of Taq PCR Mastermix (TIANGEN, Beijing, China), 6 μL of DNA template, 1 μL of primer 104F, 1 μL of primer 530R, and 17 μL of ddH 2 O. The PCR program was similar to that reported by Dong et al . and Xiao et al, with some modifications.…”

Section: Methodsmentioning

confidence: 99%

Application of Bacillus spp. in Pilot Test of Microbial Huff and Puff to Improve Heavy Oil Recovery

et al. 2017

View full text Add to dashboard Cite

Microbial metabolic products, such as biosurfactants, bioemulsifiers, acids, solvents, and biogases, are useful for reducing the viscosity of heavy oils and enhancing oil recovery. Two heavy oil viscosity-reducing microorganisms, namely, SH-2 and SH-3, were selected from produced water which were collected from high-temperature reservoirs by enrichment culture technique. The screened bacteria produce biosurfactants and biogases that can biodegrade heavy crude oil components. The screened bacteria combined with indigenous bacteria were applied in a pilot test of microbial huff and puff. Temperature, porosity, and permeability of the reservoir were 50 °C, 14.32%, and 22 mD, respectively. After microbial treatment, the 50 °C degassing for crude oil viscosity of the produced oil was decreased from 750 to 634 mPa•s. Moreover, wax and resin−asphaltene contents of produced oil were reduced by 12.3% and 16.9%, respectively. The average oil production was improved from 2.2 to 3.5 t/day after microbial treatment. The production remained stable without the chemical viscosity reducer for 54 days. The analysis of bacterial community structure indicated that the number of bacteria species increased and that the microbial diversity was highly abundant. However, harmful microorganisms for microbe-enhanced oil recovery, such as sulfate-reducing bacteria, are inhibited during the progress of microbial huff and puff.

show abstract

Biostimulation of biogas producing microcosm for enhancing oil recovery in low-permeability oil reservoir

Abstract: Indigenous microbial enhanced oil recovery (IMEOR) has been successfully applied in conventional oil reservoirs, however the mechanism in low-permeability oil reservoirs is still misunderstood.

Cited by 8 publications

References 48 publications

Composition of Bacterial and Archaeal Communities in an Alkali-Surfactant-Polyacrylamide-Flooded Oil Reservoir and the Responses of Microcosms to Nutrients

Composition of Bacterial and Archaeal Communities in an Alkali-Surfactant-Polyacrylamide-Flooded Oil Reservoir and the Responses of Microcosms to Nutrients

An Exogenous Surfactant-Producing Bacillus subtilis Facilitates Indigenous Microbial Enhanced Oil Recovery

Application of Bacillus spp. in Pilot Test of Microbial Huff and Puff to Improve Heavy Oil Recovery

Contact Info

Product

Resources

About