Biosurfactant production by <i>Mucor circinelloides</i> using apple peel, vegetable oil and corn steep liquor as substrate

Acioly, Leonila Maria Leandro; Silveira, Aline Alves Barbosa da; Anjos, Mayara Nunes Vitor; Silva, G. K. B.; Leite, Marcela Vieira; Okada, Kaoru; Campos-Takaki, Galba Maria de

doi:10.1142/9789814405041_0069

Cited by 4 publications

(3 citation statements)

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“…Kiran et al [ 30 ] reported that an Aspergillus ustus isolate showed optimal biosurfactant production at pH 7. Mucor circinelloides was found to maximally produce biosurfactant at pH 8 using apple peel, vegetable oil, and corn water as substrates [ 31 ]. The time required for maximum biosurfactant production by Penicillium sp.…”

Section: Discussionmentioning

confidence: 99%

Production of Biosurfactants by Soil Fungi Isolated from the Amazon Forest

Sena

Sanches

Rocha

et al. 2018

International Journal of Microbiology

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Biosurfactants are surface-active compounds that have sparked interest in recent years because of their environmental advantages over conventional surfactants. The aim of this study was to investigate the production of biosurfactants by soil fungi isolated from the Amazon forest. Fungi colonies were isolated from soil samples and screened for biosurfactant production in submerged fermentation. In addition, the influences of bioprocess factors (carbon source, nitrogen source, pH, and fermentation time) were investigated. Finally, the biosurfactant produced was semipurified and submitted to stability tests. One hundred fungal cultures were obtained from the soil samples, identified by micromorphology, and submitted to screening for biosurfactant production. Sixty-one strains produced biosurfactants. The strain Penicillium 8CC2 showed the highest emulsification index (54.2%). The optimized bioprocess conditions for biosurfactant production by Penicillium 8CC2 were as follows: soybean oil, 20 g/L; yeast extract, 30 g/L; pH 9; duration of 9 days. The semipurified biosurfactant showed stability after heating at 100°C for 60 min and after the addition of 30% NaCl (w/v). Tween 80 (0.2% w/v), a conventional surfactant, was used as the control.

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Section: Discussionmentioning

confidence: 99%

Production of Biosurfactants by Soil Fungi Isolated from the Amazon Forest

Sena

Sanches

Rocha

et al. 2018

International Journal of Microbiology

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“…The reduction in surface tension is commonly used as a primary criterion for selecting biosurfactant-producing microorganisms [2, 27, 28]. In this study, surface tension was used as response variable, and the effects of independent variables (lactose, CWW and CO concentrations) as well as the interactions of them, were analyzed by estimated effects represented in Table 3 and Fig.…”

Section: Resultsmentioning

confidence: 99%

Sustainable biosurfactant produced by Serratia marcescens UCP 1549 and its suitability for agricultural and marine bioremediation applications

et al. 2019

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BackgroundBiosurfactants are surface-active agents produced by microorganisms that have higher efficiency and stability, lower toxicity and higher biocompatibility and biodegradability than chemical surfactants. Despite its properties and potential application in a wide range of environmental and industrial processes, biosurfactants are still not cost-competitive when compared to their synthetic counterparts. Cost effective technologies and renewable raw substrates as agro-industrial and regional waste from northeast of Brazil as cassava flour wastewater, supplemented with lactose and corn oil are mainly the chemically media for growing microorganism and in turn the production of the biosurfactant of quality. This study aimed to obtained biosurfactant by Serratia marcescens UCP 1549 containing cassava flour wastewater (CWW), by application of a full-factorial design, as sustainable practices in puts the production process in promising formulation medium. The characterization of the biomolecule was carried out, as well as the determination of its stability and toxicity for cabbage seeds. In addition, its ability to stimulate seed germination for agriculture application and oil spill bioremediation were investigated.ResultsSerratia marcescens showed higher reduction of surface tension (25.92 mN/m) in the new medium containing 0.2% lactose, 6% cassava flour wastewater and 5% corn waste oil, after 72 h of fermentation at 28 °C and 150 rpm. The substrate cassava flour wastewater showed a promising source of nutrients for biosurfactant production. The isolate biosurfactant exhibited a CMC of 1.5% (w/v) and showed an anionic and polymeric structure, confirmed by infrared spectra. The biomolecule demonstrated high stability under different temperatures, salinity and pH values and non-toxicity against to cabbage seeds. Thus, exploring biosurfactant their potential role in seeds germinations and the promotion and agricultural applications was investigated. In addition, the effectiveness of biosurfactant for removal burned motor oil adsorbed in sand was verified.ConclusionsThe use of medium containing CWW not only reduces the cost of process of biosurfactant production, but also the environmental pollution due to the inappropriate disposal of this residue. This fact, added to the high stability and non-toxicity of the biosurfactant produced by S. marcescens UCP 1549, confirms its high environmental compatibility, make it a sustainable biocompound that can be replace chemical surfactants in diverse industries. In addition, the effectiveness of biosurfactant for stimulate seed germination and removing burned motor oil from sand, suggests its suitability for agriculture and bioremediation applications.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-1046-0) contains supplementary material, which is available to authorized users.

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“…Interestingly, for some mould species, including Mucor sp., it has been described that they can produce biosurfactants, compounds of microbial origin that exhibit surfactant properties. These biosurfactants are specifically interesting in the light of phytosterol conversion, as phytosterols are difficult to solubilize, highly hydrophobic substrates that are as such difficult to access enzymatically. The industrial large‐scale, biological production of steroids from phytosterols therefore uses chemical surfactants such as ethoxylated sorbitan esters (Tween 60 or 80®) to create micro‐emulsions, enhancing phytosterol solubilization and availability.…”

Section: Discussionmentioning

confidence: 99%

Mouldy feed: A possible explanation for the excretion of anabolic‐androgenic steroids in horses

Decloedt

Bailly-Chouriberry²,

Bussche

et al. 2016

Drug Testing and Analysis

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To ensure fair competition and to protect the horse's welfare, horses have to compete on their own merits, without any unfair advantage that might follow the use of drugs. Therefore, regulatory authorities list all substances that are not allowed in competition, including most anabolic-androgenic steroids. As zero-tolerance is retained, the question arose whether the consumption of mouldy feed could lead to the excretion of steroids, due to the biotransformation of plant phytosterols to steroids. A rapid ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analytical method, previously validated according to AORC (Association of Official Racing Chemists) and EC (European Commission) guidelines, was used to measure steroids in different sample types. Multiple mouldy feed samples were tested for the presence of steroids. The effect of digestion was tested by in vitro simulation of the horse's hindgut in batch incubations. In most feed samples no steroids were detected, even when the products were mouldy. Mouldy corn however showed to contain up to 3.0 ± 0.4 μg/kg AED (4-androstenedione), the main testosterone precursor. This concentration increased when mouldy corn (with added phytosterols) was digested in vitro. An herbal phytosupplement also showed to contain α-testosterone. These results demonstrate that it is important to caution against the consumption of any feed or (herbal) supplement of which the detailed ingredients and quantitative analysis are unknown. The consumption of mouldy corn should especially be avoided, not only from a horse health and welfare point of view, but also to avoid possible inadvertent positive doping results.

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Biosurfactant production by Mucor circinelloides using apple peel, vegetable oil and corn steep liquor as substrate

Cited by 4 publications

References 0 publications

Production of Biosurfactants by Soil Fungi Isolated from the Amazon Forest

Production of Biosurfactants by Soil Fungi Isolated from the Amazon Forest

Sustainable biosurfactant produced by Serratia marcescens UCP 1549 and its suitability for agricultural and marine bioremediation applications

Mouldy feed: A possible explanation for the excretion of anabolic‐androgenic steroids in horses

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