Biosynthèse des protéines de réserve et formation des corps protéiques dans la graine de lupin jaune (Lupinus luteus L, Légumineuses)

Proteomics for exploiting diversity of lupin seed storage proteins and their use as nutraceuticals for health and welfare

“…subunit composition and oligomeric status) and post-translational modifications (PTMs). Most of these studies have been focused on white and narrow-leafed lupins ( [22,31], and others below), and only a few were conducted on the other edible lupin, such as L. luteus [32] and L. mutabilis [7]. Main features of lupin conglutin genes and proteins are summarized in Table 1.…”

“…However, as seen above, almost all the canonical proteomic studies on these important bioactive compounds were conducted on only very few varieties from the two Mediterranean lupins, L. albus and L. angustifolius, which left nearly unexplored both their widely cultivated and naturally available germplasms, and the huge natural lupin diversity adapted to various ecogeographical conditions in the Old and the New World. The few interspecific comparative analyses, previously performed on seed storage proteins, resulted in the same pattern of conglutin families (α, β, γ, δ) [32,119,120]. Nevertheless, these analyses also revealed a great diversity in polypeptide electrophoretic patterns of total denatured and reduced conglutins [120][121][122].…”

“…4), almost all the other conglutin-polypeptide chains with which they are associated were polymorphic among lupins. The latter mostly results from post-translational transformations of polypeptideprecursors [24,[32][33][34]37,120], rather than from a large number of genes, as recently demonstrated by Foley et al [93] from analyses of conglutins across some lupin species using transcriptomic, protein and comparative genomic approaches. At a lower level, such polypeptide heterogeneity was also observed at the intraspecific level in Mediterranean lupins, as can be seen, for example, among natural populations in the poorly studied L. micranthus (Fig.…”