Comparative study of seed albumins in the Old-WorldLupinus species (Fabaceae) by reversed-phase HPLC

Salmanowicz, Bolesław P.

doi:10.1007/bf00982317

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…Their 2C-value, of a moderate level, was distinctly lower than those of the Luteus and Angustifolius sections, and higher than the Albus section species. Morphological, genetic, serological and other biochemical evidence (Gladstones, 1974;Plitmann, 1981;Plitmann and Heyn, 1984;Cristofolini, 1989;Wolko and Weeden, 1990a;Carstairs et al, 1992;Salmanowicz and Przybylska, 1994;Salmanowicz, 1995), as well as molecular phylogeny (Ka Èss and Wink, 1997), showed the Scabrispermae to be an unusually homogenous, closely related evolutionary group, contrary to the wide diversity in their chromosome numbers. Based on DNA content analysis, the rough-seeded species form a clearly distinct, close group among the Old World lupins.…”

Section: Resultsmentioning

confidence: 99%

Nuclear DNA Content Variation and Species Relationships in the Genus Lupinus (Fabaceae)

Naganowska

Wolko²,

Śliwińska³

et al. 2003

Annals of Botany

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The 2C nuclear DNA content has been estimated by flow cytometry in 18 species and botanical forms of the genus Lupinus (family Fabaceae), using propidium iodide as a fluorescent dye. They represented distinct infrageneric taxonomic groups and differed in somatic chromosome numbers. Estimated 2C DNA values ranged from 0.97 pg in L. princei to 2.44 pg in L. luteus, which gives a more than 2.5-fold variation. Statistical analysis of the data obtained resulted in a grouping that supports the generally accepted taxonomic classification of the Old World lupins. The rough-seeded L. princei turned out to be an interesting exception, getting closer to smooth-seeded species. Results of DNA content analyses are discussed with regards to the phylogenetic relationships among the Old World lupins and some aspects of the evolution of the genus.

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Section: Resultsmentioning

confidence: 99%

Nuclear DNA Content Variation and Species Relationships in the Genus Lupinus (Fabaceae)

Naganowska

Wolko²,

Śliwińska³

et al. 2003

Annals of Botany

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“…Chromatographic analysis of protein has been carried out for various types of agro foods, such as oat ( Lapvetelainen et al., 1995 ), maize ( Wilson, 1991 ), and cocoa seed ( Voigt et al., 1994 ) at 210 nm; soybean ( Oomah et al., 1994 ), whey ( Sturaro et al., 2016 ), milk ( Baranyi et al., 1995 ), and sunflower seed ( Kortt and Caldwell, 1990 ) at 214 nm; milk ( Chabance et al., 1995 ) and pea ( Chambers et al., 1992 ) at 220 nm; lupinus spices ( Salmanowicz, 1995 ), peanut seed ( Basha, 1988 ), and milk ( Baranyi et al., 1995 ) at 280 nm. Based on previous research, in this study, several types of detector (nm) were selected for the HPLC analysis.…”

Section: Methodsmentioning

confidence: 99%

Near-infrared hyperspectral imaging for online measurement of the viability detection of naturally aged watermelon seeds

Yasmin

Ahmed²,

Wakholi³

et al. 2022

Front. Plant Sci.

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The viability status of seeds before sowing is important to farmers as it allows them to make yield predictions. Monitoring the seed quality in a rapid and nondestructive manner may create a perfect solution, especially for industrial sorting applications. However, current offline laboratory-based strategies employed for the monitoring of seed viability are time-consuming and thus cannot satisfy industrial needs where there is a substantial number of seeds to be analyzed. In this study, we describe a prototype online near-infrared (NIR) hyperspectral imaging system that can be used for the rapid detection of seed viability. A wavelength range of 900–1700 nm was employed to obtain spectral images of three different varieties of naturally aged watermelon seed samples. The partial least square discriminant analysis (PLS-DA) model was employed for real-time viability prediction for seed samples moving through a conveyor unit at a speed of 49 mm/sec. A suction unit was further incorporated to develop the online system and it was programmatically controlled to separate the detected viable seeds from nonviable ones. For an external validation sample set showed classification accuracy levels of 91.8%, 80.7%, and 77.8% in relation to viability for the three varieties of watermelon seed with healthy seedling growth. The regression coefficients of the classification model distinguished some chemical differences in viable and nonviable seed which was verified by the chromatographic analysis after the detection of the proposed online system. The results demonstrated that the developed online system with the viability prediction model has the potential to be used in the seed industry for the quality monitoring of seeds.

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“…However, the classification is not entirely adequate for conglutin /3, which, in contrast to conglutin 0" remains quite soluble when all the salt is removed at neutral pH, although it precipitates at pH 4.5 (Blagrove and Gillespie, 1975). Yet another of the proteins, conglutin 0, referred to as an albumin in early studies, is again seemingly extracted quantitatively only by salt (Restani et aI., 1981: Lilley, 1986a), but remains soluble on dialysis of the salt extract against water; more recent studies have shown that conglutin 0 may indeed be extracted by water alone (Salmanowicz andPrzybylska, 1994: Salmanowicz, 1995) and thus behaves more like a classical albumin. However, low ionic strength and/or extremes of pH have been shown to lead to irreversible dissociation or denaturation for certain components (Blagrove and Gillespie, 1975): maintaining native structure is obviously important where a critical study of solubility and subunit structure is to be undertaken.…”

Section: Classification Based On Solubilitymentioning

confidence: 96%

Lupin Globulins

Lilley¹

1999

Seed Proteins

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Comparative study of seed albumins in the Old-WorldLupinus species (Fabaceae) by reversed-phase HPLC

Cited by 6 publications

References 13 publications

Nuclear DNA Content Variation and Species Relationships in the Genus Lupinus (Fabaceae)

Nuclear DNA Content Variation and Species Relationships in the Genus Lupinus (Fabaceae)

Near-infrared hyperspectral imaging for online measurement of the viability detection of naturally aged watermelon seeds

Lupin Globulins

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