Biosynthesis and Characterization of Extracellular Silver Nanoparticles from Streptomyces aizuneusis: Antimicrobial, Anti Larval, and Anticancer Activities

Abdelhady, Hemmat M.; Ashor, Mona A; Hazem, A; Saleh, Fayez M.; Selim, Samy; Nahhas, Nihal El; Abdel‐Hafez, Shams H.; Sayed, Samy; Hassan, Enas A.

doi:10.3390/molecules27010212

Cited by 20 publications

(12 citation statements)

References 43 publications

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“…Although there have been many reports on the various biological activities of greenly synthesized AgNPs in the past, there has been little analysis of their constituents, so the anti-inflammatory mechanism of this kind of AgNPs is rarely studied. 22,23 In this study, we found that silver only accounted for a small part of the composition of AC-AgNPs (32.71 ± 4.87%), and other antiinflammatory substances accounted for the majority. Therefore, this explains why the AgNPs synthesized by chemical or physical methods have proinflammatory effects, while AC-AgNPs have anti-inflammatory effects.…”

Section: Discussionmentioning

confidence: 51%

“…From the chemical structure point of view, they all contain abundant hydroxyl groups and unsaturated bonds, so they are also adsorbed on the silver nanoparticles as reducing agents and stabilizers during the synthesis of AC-AgNps, endowing AC-AgNps with some specific biological activity. Although there have been many reports on the various biological activities of greenly synthesized AgNPs in the past, there has been little analysis of their constituents, so the anti-inflammatory mechanism of this kind of AgNPs is rarely studied. , In this study, we found that silver only accounted for a small part of the composition of AC-AgNPs (32.71 ± 4.87%), and other anti-inflammatory substances accounted for the majority. Therefore, this explains why the AgNPs synthesized by chemical or physical methods have proinflammatory effects, while AC-AgNPs have anti-inflammatory effects.…”

Section: Discussionmentioning

confidence: 58%

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Green Biosynthesis of Silver Nanoparticles Using Aqueous Extracts of Ageratum Conyzoides and Their Anti-Inflammatory Effects

Zha

et al. 2023

ACS Appl. Mater. Interfaces

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The NLRP3 inflammasome, which plays a central role in innate immunity, is linked to a variety of inflammatory diseases, and thus it may provide a new target for the treatment of those diseases. Biosynthesized silver nanoparticles (AgNPs), particularly those synthesized using medicinal plant extracts, have recently been shown to be a promising therapeutic option. Herein, the aqueous extract of Ageratum conyzoids was used to prepare a series of sized AgNPs (AC-AgNPs), in which the smallest mean particle size was 30 ± 1.3 nm with a polydispersity of 0.328 ± 0.009. The ζ potential value was −28.77 with a mobility of −1.95 ± 0.24 cm2/(v·s). Its main ingredient, elemental silver, accounted for about 32.71 ± 4.87% of its mass, and other ingredients included amentoflavone-7,7⁗-dimethyl ether, 1,3,5-tricaffeoylquinic acid, kaempferol 3,7,4′-triglucoside, 5,6,7,3′,4′,5′-hexamethoxyflavone, kaempferol, and ageconyflavone B. In LPS+ATP-stimulated RAW 264.7 and THP-1 cells, AC-AgNPs significantly inhibited the release of IL-1β, IL-18, TNF-α, and caspase-1, indicating that AC-AgNPs can inhibit the activation of the NLRP3 inflammasome. The mechanistic study revealed that AC-AgNPs could decrease the phosphorylation levels of IκB-α and p65, resulting in decreased expression of NLRP3 inflammasome-related proteins, including pro-IL-1β, IL-1β, procaspase 1, caspase 1P20, NLRP3, and ASC, and also scavenge the level of intracellular ROS to prevent NLRP3 inflammasome assembly. Furthermore, AC-AgNPs attenuated the in vivo expression of inflammatory cytokines by suppressing NLRP3 inflammasome activation in a peritonitis mouse model. Our study provides evidence that the as-prepared AC-AgNPs can inhibit the inflammatory process by suppressing NLRP3 inflammasome activation and might be used to treat NLRP3 inflammasome-driven inflammatory diseases.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 58%

Green Biosynthesis of Silver Nanoparticles Using Aqueous Extracts of Ageratum Conyzoides and Their Anti-Inflammatory Effects

Zha

et al. 2023

ACS Appl. Mater. Interfaces

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“…The cytotoxicity of green synthesized CMBNPs was determined by using a NRU colorimetric assay. This assay is based on the capacity of live cells to bind and integrate neutral red into lysosomes [ 29 ]. The cytotoxicity was measured as a concentration-dependent reduction in the uptake of neutral red in response to CMBNP exposure and serves as a sensitive indicator of both cell integrity and growth inhibition, determining IC 50 (50% inhibiting concentration).…”

Section: Resultsmentioning

confidence: 99%

Biogenic Synthesis of Cu-Mn Bimetallic Nanoparticles Using Pumpkin Seeds Extract and Their Characterization and Anticancer Efficacy

et al. 2023

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Background: Cancer is a chronic, heterogeneous illness that progresses through a spectrum of devastating clinical manifestations and remains the 2nd leading contributor to global mortality. Current cancer therapeutics display various drawbacks that result in inefficient management. The present study is intended to evaluate the anticancer potential of Cu-Mn bimetallic NPs (CMBNPs) synthesized from pumpkin seed extract against colon adenocarcinoma cancer cell line (HT-29). Methods: The CMBNPs were biosynthesized by continuously stirring an aqueous solution of pumpkin seed extract with CuSO4 and manganese (II) acetate tetrahydrate until a dark green solution was obtained. The characteristic features of biogenic CMBNPs were assessed by UV-visible spectrophotometry (UV-vis), X-ray powder diffraction (XRD), energy-dispersive X-ray (EDX), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). A battery of biological assays, viz. neutral red uptake (NRU) assay, in vitro scratch assay, and comet assay, were performed for anticancer efficacy evaluation. Results: The formation of spherical monodispersed bimetallic nanoparticles with an average size of 50 nm was recorded using TEM. We observed dose-dependent cytotoxicity of CMBNPs in the HT-29 cell line with an IC50 dose of 115.2 µg/mL. On the other hand, CMBNPs did not show significant cytotoxicity against normal cell lines (Vero cells). Furthermore, the treatment of CMBNPs inhibited the migration of cancer cells and caused DNA damage with a significant increase in comet tail length. Conclusions: The results showed substantial anticancer efficacy of CMBNPs against the studied cancer cell line. However, it is advocated that the current work be expanded to different in vitro cancer models so that an in vivo validation could be carried out in the most appropriate cancer model.

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“…The experiment was performed in triplicates, and the assay was read at 492 nm. The cytotoxicity (%) was evaluated using Equation (2) [12,13]:…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

Antiproliferative Activity of PEG-PEI-SWCNTs against AMJ13 Breast Cancer Cells

Jwameer

Salman

Noori

et al. 2023

Journal of Nanomaterials

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Single-walled carbon nanotubes (SWCNTs) involving polyethylene glycol (PEG) and polyethyleneimine (PEI), which contain amines, were produced and analyzed using X-ray diffraction (XRD), UV–vis spectroscopy, Raman spectroscopy (RS), and transmission electron microscopy (TEM) for the spectral and structural analyses of PEG-PEI-SWCNTs. PEG-PEI-SWCNTs demonstrated peaks using UV–vis spectroscopy at 300.98 nm, have a broad peak at 2θ = 23.51, linked to 002 with d-spacing (3.7816), and have crystalline sizes of 8.48 nm. In PEG-PEI-SWCNTs, TEM images demonstrated the accumulation of SWCNTs with PEG-PEI due to an increased main grain size with functionalization to 80.68 nm. The D and G bands in PEG-PEI-SWCNTs moved to 1,292 and 1,586 cm−1, respectively. SWCNTs are observed as a bundle of inhomogeneous, long-curved accumulates containing multitudes of tubes. The PEG-PEI-SWCNTs have a tubular structure that demonstrates particles scattered alongside the carbon nanotube sidewalls, which indicate that PEG and PEI are conjugated to the SWCNTs. Cytotoxicity evaluation of PEG-PEI-SWCNTs (6.25–100 µg/mL) in the breast cancer AMJ13 cell line was conducted for 24 and 72 hr, and the results showed enhanced toxicity to the tumor cells and decreased cytotoxicity against rhabdomyosarcoma (RD) normal cells.

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Biosynthesis and Characterization of Extracellular Silver Nanoparticles from Streptomyces aizuneusis: Antimicrobial, Anti Larval, and Anticancer Activities

Cited by 20 publications

References 43 publications

Green Biosynthesis of Silver Nanoparticles Using Aqueous Extracts of Ageratum Conyzoides and Their Anti-Inflammatory Effects

Green Biosynthesis of Silver Nanoparticles Using Aqueous Extracts of Ageratum Conyzoides and Their Anti-Inflammatory Effects

Biogenic Synthesis of Cu-Mn Bimetallic Nanoparticles Using Pumpkin Seeds Extract and Their Characterization and Anticancer Efficacy

Antiproliferative Activity of PEG-PEI-SWCNTs against AMJ13 Breast Cancer Cells

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