Biosynthesis and characterization of novel polyhydroxyalkanoate polymers with high elastic property by Cupriavidus necator PHB−4 transformant

Chia, Kim-Hou; Ooi, Tin-Fong; Saika, Azusa; Tsuge, Takeharu; Sudesh, Kumar

doi:10.1016/j.polymdegradstab.2010.09.011

Cited by 26 publications

(11 citation statements)

References 28 publications

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“…However, the molecular weights of PHA produced through bacterial fermentations are strongly influenced by its producer (i.e. bacterial strain, PHA synthase and substrate specificity) and its culture conditions . Besides, fermentation conditions such as incubation time, pH and aeration also greatly influence the molecular weight of PHAs …”

Section: Resultsmentioning

confidence: 99%

“…This suggested that the bacterium showed higher substrate specificity towards increasing concentrations of 1,6‐hexanediol, thus leading to higher polymer chain initiations which resulted in decreased copolymers molecular weight. Several studies have demonstrated that copolymer composition, molecular weight and polydispersities of the copolymers are controlled by the PHA synthase activity . Gerngross and Martin demonstrated that the molecular weight of P(3HB) synthesized by in vitro polymerization using purified PHA synthase from R .…”

Section: Resultsmentioning

confidence: 99%

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Synthesis of P(3HB‐co‐4HB) copolymer with target‐specific 4HB molar fractions using combinations of carbon substrates

Iqbal

Amirul

2013

J of Chemical Tech & Biotech

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Synthesis of P(3HB‐co‐4HB) copolymer with target‐specific 4HB molar fractions using combinations of carbon substrates

Iqbal

Amirul

2013

J of Chemical Tech & Biotech

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“…The authors of another study investigated copolymers with a considerably lower molar fraction of 3H4MV (0.8–3.1 mol%) and found that their M n ranged between 98 and 251 kDa, with polydispersity values being rather low (1.6–1.9). Higher values of M n (298–421 kDa) and Ð (2.2–2.5) were reported by Chia et al . for P(3HB‐ co ‐3H4MV‐ co ‐3HHx) and P(3HB‐ co ‐3 HV‐ co ‐3H4MV‐ co ‐3HHx) specimens with low molar fractions of 3H4MV (1–3 mol%); the authors, though, concluded that incorporation of methylvalerate monomers decreased the molecular weight of the polymer relative to P(3HB) and P(3HB‐ co ‐3 HV).…”

Section: Resultsmentioning

confidence: 98%

Biosynthesis and properties of P(3HB‐co‐3HV‐co‐3H4MV) produced by using the wild‐type strain Cupriavidus eutrophus B‐10646

Volova

Меньшикова

Zhila

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Polyhydroxyalkanoates (PHA) containing 3‐hydroxy‐4‐methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate‐co‐3‐hydroxy‐4‐methylvalerate) [P(3HB‐co‐3 HV‐co‐3H4MV)] by Cupriavidus eutrophus B‐10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS It was shown that 4‐methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L−1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years. © 2018 Society of Chemical Industry

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“…1, leaving the cell debris and the supernatant was then concentrated through rotary evaporation and the polymer precipitated by dropping it in cold methanol. The excess methanol was then removed and the polymer allowed to dry in a petri dish under a fume hood until pure PHA (white powder) was observed (Chia et al 2010). …”

Section: Freeze-dried Cell and Pure Phamentioning

confidence: 99%

Effect of Microwave Pretreatment on the Properties of Particleboard Made from Para Rubber Wood Sawdust with the Addition of Polyhydroxyalkanoates

Iewkittayakorn¹,

Khunthongkaew²,

Chotigeat³

et al. 2017

JSM

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The aim of this study was to investigate the effect of microwave pretreatment on the properties of particleboard made from para rubber wood sawdust with the addition of polyhydroxyalkanoates (PHAs). Para wood sawdust was pretreated with microwaves at different wattages (400, 600 and 800 W) Kata kunci: Habuk gergaji kayu getah para; ketuhar gelombang mikro; papan partikel; PHA; polihidroksialkanoat sawdust or rubber wood dust which is a major by-product of cutting, grinding, drilling or otherwise pulverizing wood with a saw or other mechanical device can also be considered as a new source of raw materials that can replace rubber wood in manufacturing panels. Moreover, the utilization of sawdust could help to reduce the amount of wood from forest which is wasted, by using the wasted products as a raw material in the production of valuable products such as particleboard. Particleboard is widely used for making furniture, interior decoration and appliances.

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Biosynthesis and characterization of novel polyhydroxyalkanoate polymers with high elastic property by Cupriavidus necator PHB−4 transformant

Cited by 26 publications

References 28 publications

Synthesis of P(3HB‐co‐4HB) copolymer with target‐specific 4HB molar fractions using combinations of carbon substrates

Synthesis of P(3HB‐co‐4HB) copolymer with target‐specific 4HB molar fractions using combinations of carbon substrates

Biosynthesis and properties of P(3HB‐co‐3HV‐co‐3H4MV) produced by using the wild‐type strain Cupriavidus eutrophus B‐10646

Effect of Microwave Pretreatment on the Properties of Particleboard Made from Para Rubber Wood Sawdust with the Addition of Polyhydroxyalkanoates

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